Overexpression of the PP2A regulatory subunit Tap46 leads to enhanced plant growth through stimulation of the TOR signalling pathway

Ahn, Chang Sook; Ahn, Hee-Kyung; Pai, Hyun Sook

doi:10.1093/jxb/eru438

Cited by 72 publications

(66 citation statements)

References 61 publications

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“…MRF1 phosphorylation did not occur without the addition of S6K1 (Supplemental Figure 18A). Arabidopsis S6K1 has a conserved site, T449, which is phosphorylated by the TOR kinase (Xiong and Sheen, 2012;Ahn et al, 2015). We determined MRF1 phosphorylation activities of two mutant forms of S6K1, a phospho-null mutant (T449A) and a phospho-mimetic mutant (T449D), and compared them to normal S6K1 ( Figure 11C).…”

Section: Mrf1 Is Phosphorylated By S6k1 and S6k2 In Vitromentioning

confidence: 99%

“…Agrobacterium-mediated transient expression was performed using Agrobacterium C58C1 strain as described (Ahn et al, 2015), except for BiFC experiments that used the GV3101 strain. Overnight-grown Agrobacterium culture was resuspended in infiltration medium (10 mM MES-KOH, pH 5.7, 10 mM MgSO 4 , and 500 mM acetosyringone) to different OD 600 depending on experiments (described below) and incubated for 2 h at room temperature, before infiltration into Nicotiana benthamiana leaves.…”

Section: Agrobacterium-mediated Transient Expressionmentioning

confidence: 99%

“…Arabidopsis eIF4A-1 and eIF4E-1 CDSs were cloned into pSPYCE using SpeI/SmaI and XbaI/XhoI sites, respectively. Construction of pSPYCE-S6K1 and pSPYCE-S6K2 was previously described (Ahn et al, 2015). Agrobacterium strains containing the pSPYNE, pSPYCE, and 35S:p19 construct were coinfiltrated at the OD 600 ratio of 1:1:1.5 into leaves of 3-week-old N. benthamiana plants.…”

Section: Bifcmentioning

confidence: 99%

“…CDSs of the MRF genes were cloned into the pCAMBIA1390-3xFlag vector using SalI and EcoRI sites. Construction of pCAMBIA1390-S6K1:Myc and pCAMBIA1390-S6K2:Myc was previously described (Ahn et al, 2015). Agrobacterium strains containing each pCAMBIA construct and 35S:p19 construct was coinfiltrated into 4-weekold N. benthamiana leaves at the OD 600 ratio of 1.0:1.0:1.5.…”

Section: Coimmunoprecipitationmentioning

confidence: 99%

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MRF Family Genes Are Involved in Translation Control, Especially under Energy-Deficient Conditions, and Their Expression and Functions Are Modulated by the TOR Signaling Pathway

et al. 2017

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Dynamic control of protein translation in response to the environment is essential for the survival of plant cells. Target of rapamycin (TOR) coordinates protein synthesis with cellular energy/nutrient availability through transcriptional modulation and phosphorylation of the translation machinery. However, mechanisms of TOR-mediated translation control are poorly understood in plants. Here, we report that Arabidopsis thaliana MRF (MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR) family genes encode translation regulatory factors under TOR control, and their functions are particularly important in energy-deficient conditions. Four MRF family genes (MRF1-MRF4) are transcriptionally induced by dark and starvation (DS). Silencing of multiple MRFs increases susceptibility to DS and treatment with a TOR inhibitor, while MRF1 overexpression decreases susceptibility. MRF proteins interact with eIF4A and cofractionate with ribosomes. MRF silencing decreases translation activity, while MRF1 overexpression increases it, accompanied by altered ribosome patterns, particularly in DS. Furthermore, MRF deficiency in DS causes altered distribution of mRNAs in sucrose gradient fractions and accelerates rRNA degradation. MRF1 is phosphorylated in vivo and phosphorylated by S6 kinases in vitro. MRF expression and MRF1 ribosome association and phosphorylation are modulated by cellular energy status and TOR activity. We discuss possible mechanisms of the function of MRF family proteins under normal and energy-deficient conditions and their functional link with the TOR pathway.

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Section: Mrf1 Is Phosphorylated By S6k1 and S6k2 In Vitromentioning

confidence: 99%

Section: Agrobacterium-mediated Transient Expressionmentioning

confidence: 99%

Section: Bifcmentioning

confidence: 99%

Section: Coimmunoprecipitationmentioning

confidence: 99%

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MRF Family Genes Are Involved in Translation Control, Especially under Energy-Deficient Conditions, and Their Expression and Functions Are Modulated by the TOR Signaling Pathway

et al. 2017

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“…In plants, PP2As play crucial roles in hormone signal transduction and abiotic stress response (DeLong, 2006). Overexpression of some PP2As can enhance plant growth and increase drought tolerance in transgenic plants (Luo et al, 2006;Xu et al, 2007;Ahn et al, 2015). However, another study reported a contradictory result for the role of PP2A by overexpressing and knocking out the C1 subunit gene in Arabidopsis (Pernas et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a serine/threonine protein phosphatase2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam.

Xu¹,

Yong²,

Shao³

et al. 2017

Turk J Biol

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Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. Characterization of PP2A can elucidate the mechanisms of stress resistance in this crop. In this study, a total of 15 PP2A transcripts, including nine regulatory subunit-encoding sequences and six catalytic subunit-encoding sequences, were identified from sweet potato and found to be expressed at varying levels. Only one contained a complete open reading frame and encoded a B regulatory subunit (termed IbPP2A1). Ten polymorphic sites were distributed in the coding region of this gene, but most did not result in amino acid change. RNA sequencing-based digital gene expression profiling showed that this PP2A gene was primarily expressed in fibrous roots and developing tuberous roots under natural conditions. After drought, salinity, and alkaline stress treatment, the expression of IbPP2A1 was obviously upregulated in stems and tuberous roots at 2 days, whereas the expression of IbPP2A1 in leaves was only upregulated by drought stress at 2 days. However, heterogeneous expression of IbPP2A1 did not enhance abiotic stress tolerance in recombinant Saccharomyces cerevisiae. The results described here indicate that IbPP2A1 is an abiotic stress-responsive gene, but it could not work alone in vitro. This study provides a preliminary but global insight into PP2A proteins in sweet potato for further investigations on improving stress tolerance in this crop.

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Proteomic and metabolomic analysis of Nicotiana benthamiana under dark stress

Shen

Chen

et al. 2021

FEBS Open Bio

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Exposure to extended periods of darkness is a common source of abiotic stress that significantly affects plant growth and development. To understand how Nicotiana benthamiana responds to dark stress, the proteomes and metabolomes of leaves treated with darkness were studied. In total, 5763 proteins and 165 primary metabolites were identified following dark treatment. Additionally, the expression of autophagy-related gene (ATG) proteins was transiently upregulated. Weighted gene coexpression network analysis (WGCNA) was utilized to find the protein modules associated with the response to dark stress. A total of four coexpression modules were obtained. The results indicated that heat-shock protein (HSP70), SnRK1-interacting protein 1, 2A phosphatase-associated protein of 46 kDa (Tap46), and glutamate dehydrogenase (GDH) might play crucial roles in N. benthamiana's response to dark stress. Furthermore, a protein-protein interaction (PPI) network was constructed and top-degreed proteins were predicted to identify potential key factors in the response to dark stress. These proteins include isopropylmalate isomerase (IPMI), eukaryotic elongation factor 5A (ELF5A), and ribosomal protein 5A (RPS5A). Finally, metabolic analysis suggested that some amino acids and sugars were involved in the dark-responsive pathways. Thus, these results provide a new avenue for understanding the defensive mechanism against dark stress at the protein and metabolic levels in N. benthamiana.The direct effect of illumination on plants is to carry out photosynthesis, so that organic material could be produced for plants to store energy. Illumination can also be used as a signal factor to regulate plant growth and development, including all stages of plant growth [1]. Lack of illumination is likely to lead to

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Overexpression of the PP2A regulatory subunit Tap46 leads to enhanced plant growth through stimulation of the TOR signalling pathway

Abstract: HighlightOur study of Tap46 overexpression suggests that Tap46 enhances plant growth as a positive effector of the TOR signalling pathway. Furthermore, the abundance of Tap46/PP2Ac protein is regulated by TOR activity.

Cited by 72 publications

References 61 publications

MRF Family Genes Are Involved in Translation Control, Especially under Energy-Deficient Conditions, and Their Expression and Functions Are Modulated by the TOR Signaling Pathway

MRF Family Genes Are Involved in Translation Control, Especially under Energy-Deficient Conditions, and Their Expression and Functions Are Modulated by the TOR Signaling Pathway

Cloning and characterization of a serine/threonine protein phosphatase2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam.

Proteomic and metabolomic analysis of Nicotiana benthamiana under dark stress

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