Overexpression of the yeast transcription activator Msn2 confers furfural resistance and increases the initial fermentation rate in ethanol production

Sasano, Yu; Watanabe, Dai; Ukibe, Ken; Inai, Tomomi; Ohtsu, Iwao; Shimoi, Hitoshi; Takagi, Hiroshi

doi:10.1016/j.jbiosc.2011.11.017

Cited by 77 publications

(40 citation statements)

References 26 publications

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“…Indeed, overexpression of various aldehyde reduction enzymes encoded by genes YKL071W , ALD6 , ADH7 and ARI1 have been demonstrated to be strongly associated with furfural resistance in yeast [8,12,17]. In addition, overexpression of glucose-6-phosphate dehydrogenase encoding gene ZWF1 and transcriptional activator encoding gene MSN2 , which are involved in regeneration of NAD(P)H and stress response, respectively, have also been confirmed to increase furfural tolerance in S. cerevisiae [25,26]. To compare these reported targets with siz1Δ -associated furfural tolerance, individual overexpression of each gene was performed in strain BAD.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

Xiao

Zhao

2014

Biotechnol Biofuels

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BackgroundFurfural is a major growth inhibitor in lignocellulosic hydrolysates and improving furfural tolerance of microorganisms is critical for rapid and efficient fermentation of lignocellulosic biomass. In this study, we used the RNAi-Assisted Genome Evolution (RAGE) method to select for furfural resistant mutants of Saccharomyces cerevisiae, and identified a new determinant of furfural tolerance.ResultsBy using a genome-wide RNAi (RNA-interference) screen in S. cerevisiae for genes involved in furfural tolerance, we identified SIZ1, a gene encoding an E3 SUMO-protein ligase. Disruption of SIZ1 gene function by knockdown or deletion conferred significantly higher furfural tolerance compared to other previously reported metabolic engineering strategies in S. cerevisiae. This improved furfural tolerance of siz1Δ cells is accompanied by rapid furfural reduction to furfuryl alcohol and leads to higher ethanol productivity in the presence of furfural. In addition, the siz1Δ mutant also exhibited tolerance towards oxidative stress, suggesting that oxidative stress tolerance related proteins may be under the SUMO regulation of SIZ1p and responsible for furfural tolerance.ConclusionsUsing a genome-wide approach, we identified a novel determinant for furfural tolerance, providing valuable insights into the design of recombinant microbes for efficient lignocellulose fermentation.

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Section: Resultsmentioning

confidence: 99%

Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

Xiao

Zhao

2014

Biotechnol Biofuels

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“…161 During pretreatment of biomass, a problem is the liberation of the inhibitor furfural. However, tolerance to furfural can be achieved by overexpression of S. cerevisiae genes encoding (1) yeast transcription activator MSN2; 162 (2) ZWF1 of the pentose phosphate pathway; 163 (3) ADH1 encoding alcohol dehydrogenase 1; and (4) TAL1, encoding transaldolase 1. 164 P. stipitis can produce ethanol from C5 sugars and also clean up concentrated toxins liberated during lignocellulose degradation.…”

Section: Biofuelsmentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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where he has contributed in a major way to the reputation of this department for many years. He also served as an Adjunct Professor at the University of Geneva. Among Julian's areas of study and accomplishment are fungal toxins including α-sarcin, chemical synthesis of triterpenes, mode of action of streptomycin and other aminoglycoside antibiotics, biochemical mechanisms of antibiotic resistance in clinical isolates of bacteria harboring resistance plasmids, their origins and evolution, secondary metabolism of microorganisms, structure and function of bacterial ribosomes, antibiotic resistance mutations in yeast ribosomes, cloning of resistance genes from an antibiotic-producing microbe, gene cloning for industrial purposes, engineering of herbicide resistance in useful crops, bleomycin-resistance gene in clinical isolates of Staphylococcus aureus and many other topics. He has been an excellent teacher, lecturing in both English and French around the world, and has organized international courses. Julian has also served on the NIH study sections, as Editor for several international journals, and was one of the founders of the journal Plasmid. We expect the impact of Julian's accomplishments to continue into the future.

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“…86,87 We speculate that the function of MSN2 in K. marxianus might not relate to the inhibitors tolerance or phosphorylation of MSN2 was more important in regulating the genes in response to the multiple inhibitors stress.…”

mentioning

confidence: 93%

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Wang

Yang

et al. 2018

RSC Adv.

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During pretreatment of lignocellulosic biomass, toxic compounds were released and inhibited the growth and fermentation of microorganisms. Here the global transcriptional response of K. marxianus to multiple inhibitors including acetic acid, phenols, furfural and HMF, at 42 C, was studied, via RNA-seq technology.Genes involved in the glycolysis pathway, fatty acid metabolism, ergosterol metabolism and vitamin B6 and B1 metabolic process were enriched in the down-regulated gene set, while genes involved in TCA cycle, respiratory chain, ROS detoxification and transporter coding genes were enriched in the up-regulated gene set in response to the multiple inhibitors stress. Further real time-PCR results with three single inhibitor stress conditions showed that different transporters responded quite differently to different inhibitor stress. Coenzyme assay results showed that the level of NAD + was increased and both NADH/ NAD + and NADPH/NADP + ratio decreased. Furthermore, genes involved with transcription factors related to carbohydrate metabolism, sulfur amino acids metabolism, lipid metabolism or those directly involved in the transcriptional process were significantly regulated. Though belonging to different GO categories or KEGG pathway, many differentially expressed genes were enriched in maintaining the redox balance, NAD(P) + /NAD(P)H homeostasis or NAD + synthesis, energy production, and iron transportation or metabolism. These results suggest that engineering these aspects represents a possible strategy to develop more robust strains for industrial fermentation from cellulosic biomass.

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Overexpression of the yeast transcription activator Msn2 confers furfural resistance and increases the initial fermentation rate in ethanol production

Cited by 77 publications

References 26 publications

Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

Production of valuable compounds by molds and yeasts

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

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