Overexpression of UDP-glucuronosyltransferase and cytochrome P450 enzymes confers resistance to sulfoxaflor in field populations of the aphid, Myzus persicae

Spirotetramat resistance inMyzus persicae(Sulzer) (Hemiptera: Aphididae) and its association with the presence of theA2666Vmutation

“…In the case of sulfoxaflor, there were significant population differences ( F 2,122 = 9.21, p < 0.001). Munglinup209 was confirmed as possessing resistance, with a resistance ratio of approximately 14, which is consistent with previously published data 10 . Surprisingly, there was also a significant difference in responses to sulfoxaflor between Kyabram98 and Osborne171.…”

“…To further understand the broader resistance profile of those aphids found to possess spirotetramat resistance, we undertook bioassays against two additional insecticides: sulfoxaflor and flupyradifurone. Sulfoxaflor has been used to control aphids across the globe for more than a decade, with resistance recently discovered in a small number of Australian populations of M. persicae 10 . Flupyradifurone is a newer insecticide, targeting sucking pests 25 and has only recently been registered against M. persicae .…”

“…Sulfoxaflor has been used to control aphids across the globe for more than a decade, with resistance recently discovered in a small number of Australian populations of M. persicae. 10 Flupyradifurone is a newer insecticide, targeting sucking pests 25 and has only recently been registered against M. persicae. Sulfoxaflor bioassays were conducted using a micro-topical methodology closely following Pym et al 10 We used a proprietary formulation of sulfoxaflor 240 g L −1 (Transform®, Corteva Agriscience), dissolving the chemical product in water to generate a rate of 2400 mg L −1 , before serially diluting this stock solution in acetone to generate eight test concentrations (control [0], 0.00001, 0.0001, 0.001, 0.01, 0.1, 1, 10 ng of active ingredient [a.i.]…”

“…Each aphid received a volume of 0.25 μl directly onto the prothorax. Aphids were scored as alive, dead or incapacitated after 72 h. We tested three clones, which included Osborne171 and Kyabram98, as well as Munglinup209 (a clone from Western Australia that is known to possess low-level sulfoxaflor-resistance, mediated through the overexpression of a P450 gene CYP380C40 and the UDP-glucuronosyltransferase gene UGT344P2; see Pym et al 10 ).…”

“…This work has found widespread resistance to synthetic pyrethroids, organophosphates, carbamates and neonicotinoids across the majority of agricultural regions. More recently, low‐level resistance to the sulfoximine insecticide, sulfoxaflor, has been detected in a small number of field populations of M. persicae in the state of Western Australia 10 . Interestingly, anholocyclic clones that possess multiple resistances dominate Australian agricultural fields 9 (P. A. Umina, unpublished) and likely undergo parthenogenetic reproduction year round 11 …”