Overexpression of ZmSAG39 in maize accelerates leaf senescence in Arabidopsis thaliana

Wang, Chunlai; Chen, Nannan; Liu, Jiaqi; Jiao, Peng; Liu, Siyan; Qu, Jing; Guan, Shuyan; Ma, Yiyong

doi:10.1007/s10725-022-00874-1

Cited by 8 publications

(7 citation statements)

References 56 publications

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“…The above results were consistent with previous studies in Arabidopsis. Overexpression of ZmSAG39 in Arabidopsis also showed increased sensitivity to drought and dark stress [40].…”

Section: Discussionmentioning

confidence: 97%

“…The conductivity at this time was the final conductivity (S2). The relative electrical conductivity (REC) was calculated as follows: REC(%) = S1/S2 × 100 [40]. Three biological replications were carried out.…”

Section: Determination Of Physiological and Biochemical Activitiesmentioning

confidence: 99%

“…qRT-PCR was performed using SYBR PreMix Ex Taq (Takara, Changchun, China). The PCR program was: 95 • C for 10 min, followed by 40 cycles of 95 • C for 10 s, 60 • C for 20 s, and 72 • C for 15 s, followed by melting curve analysis [40]. The 2 −∆∆CT method was used for data analysis.…”

Section: Qrt-pcrmentioning

confidence: 99%

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A Novel Senescence-Specific Gene (ZmSAG39) Negatively Regulates Darkness and Drought Responses in Maize

Wang

Gao

Chen

et al. 2022

IJMS

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The papain-like cysteine proteases (PLCPs) is a subfamily of cysteine proteases that plays an important role in leaf senescence, and some of its members are involved in the regulation of plant growth and development under stress. In this study, we cloned a new gene, ZmSAG39, from maize. Expression profile analysis showed that ZmSAG39 was induced by darkness and drought treatments. In addition, the ZmSAG39 overexpression in maize accelerated the senescence of maize leaves under darkness and drought treatments. However, the knockout of ZmSAG39 in maize enhanced the resistance of maize to darkness and drought stresses and reduced the degree of senescence of maize leaves. Under drought stress, compared with WT plants, the knockout lines had a higher seed germination rate, seedling survival rate and chlorophyll content, and lower reactive oxygen species (ROS) level and malondialdehyde (MDA) content. In addition, quantitative real-time PCR (qRT-PCR) analysis showed that ZmSAG39 negatively regulated some stress-related genes but positively regulated senescence-related genes under darkness and drought stress conditions. To summarize, these results indicate that ZmSAG39 is a senescence-related gene and plays a negative role in response to darkness and drought stresses. This study laid a theoretical foundation for the innovation of maize germplasm resources with high quality, high yield and strong stress resistance.

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“…The above results were consistent with previous studies in Arabidopsis. Overexpression of ZmSAG39 in Arabidopsis also showed increased sensitivity to drought and dark stress [40].…”

Section: Discussionmentioning

confidence: 97%

Section: Determination Of Physiological and Biochemical Activitiesmentioning

confidence: 99%

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A Novel Senescence-Specific Gene (ZmSAG39) Negatively Regulates Darkness and Drought Responses in Maize

Wang

Gao

Chen

et al. 2022

IJMS

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“…Zm18S and AtACTIN8 were selected as the internal reference genes for maize and A. thaliana , respectively. The 2 −ΔΔCT method was used to analyze the relative expression levels of relevant genes ( Jiao et al., 2022 ; Wang C. et al., 2022 ). All primers used in this study are listed in Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

ZmLBD2 a maize (Zea mays L.) lateral organ boundaries domain (LBD) transcription factor enhances drought tolerance in transgenic Arabidopsis thaliana

et al. 2022

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Maize (Zea mays L.) is an annual gramineous herb and is among the world’s most important crop species. Drought is the main factor contributing to maize yield reduction. The lateral organ boundaries domain (LBD) proteins belong to a class of higher-plant-specific transcription factors. LBD proteins usually include the highly conserved lateral organ boundaries (LOB) domains that play essential roles in plant growth and response to biotic stresses. However, few studies have addressed the biological functions of LBD genes associated with maize response to drought. Here we cloned the ZmLBD2 gene from maize and described its role in combating drought. Investigating ZmLBD2 subcellular localization, we show that it localizes to the cell nucleus and can specifically bind with inverted repeats of “GCGGCG”. Under drought stress, Arabidopsis thaliana overexpressing ZmLBD2 performed better than the wild-type plants in terms of seed germination rates, root length, relative water content, fresh weight, chlorophyll content, proline content, and antioxidant enzyme content. Arabidopsis overexpressing ZmLBD2 contained less MDA, H2O2, and O2− than the wild-type plants. Our protein-protein interaction results indicate an interaction between the ZmLBD2 and ZmIAA5 genes. In conclusion, the ZmLBD2 gene positively regulates H2O2 homeostasis in plants, strengthening drought resistance.

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“…MDA was detected using the detection kit [71]. The contents of H 2 O 2 and O 2 − were determined according to the acetone method [72]. A reaction mixture was prepared consisting of 50 mM of potassium dihydrogen phosphate buffer, 2 mM of Na 2-EDTA, 0.1 mol/L of H 2 O 2 , and 0.2 mL of the enzyme extract for the analysis of catalase (CAT) activity.…”

Section: Determination Of Physiological and Biochemical Activitiesmentioning

confidence: 99%

The Overexpression of Zea mays Strigolactone Receptor Gene D14 Enhances Drought Resistance in Arabidopsis thaliana L.

Zhang,

Wang,

Jiao

et al. 2024

IJMS

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Strigolactones (SLs) represent a recently identified class of plant hormones that are crucial for plant tillering and mycorrhizal symbiosis. The D14 gene, an essential receptor within the SLs signaling pathway, has been well-examined in crops, like rice (Oryza sativa L.) and Arabidopsis (Arabidopsis thaliana L.), yet the research on its influence in maize (Zea mays L.) remains scarce. This study successfully clones and establishes Arabidopsis D14 gene overexpression lines (OE lines). When compared with the wild type (WT), the OE lines exhibited significantly longer primary roots during germination. By seven weeks of age, these lines showed reductions in plant height and tillering, alongside slight decreases in rosette and leaf sizes, coupled with early aging symptoms. Fluorescence-based quantitative assays indicated notable hormonal fluctuations in OE lines versus the WT, implying that D14 overexpression disrupts plant hormonal homeostasis. The OE lines, exposed to cold, drought, and sodium chloride stressors during germination, displayed an especially pronounced resistance to drought. The drought resistance of OE lines, as evident from dehydration–rehydration assays, outmatched that of the WT lines. Additionally, under drought conditions, the OE lines accumulated less reactive oxygen species (ROS) as revealed by the assessment of the related physiological and biochemical parameters. Upon confronting the pathogens Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), post-infection, fluorescence quantitative investigations showed a significant boost in the salicylic acid (SA)-related gene expression in OE lines compared to their WT counterparts. Overall, our findings designate the SL receptor D14 as a key upregulator of drought tolerance and a regulator in the biotic stress response, thereby advancing our understanding of the maize SL signaling pathway by elucidating the function of the pivotal D14 gene.

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Overexpression of ZmSAG39 in maize accelerates leaf senescence in Arabidopsis thaliana

Cited by 8 publications

References 56 publications

A Novel Senescence-Specific Gene (ZmSAG39) Negatively Regulates Darkness and Drought Responses in Maize

A Novel Senescence-Specific Gene (ZmSAG39) Negatively Regulates Darkness and Drought Responses in Maize

ZmLBD2 a maize (Zea mays L.) lateral organ boundaries domain (LBD) transcription factor enhances drought tolerance in transgenic Arabidopsis thaliana

The Overexpression of Zea mays Strigolactone Receptor Gene D14 Enhances Drought Resistance in Arabidopsis thaliana L.

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