Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Ostrowski, A; Nordmeyer,; Boreham,; Holzhausen,; Mundhenk,; Graf, Laura; Meinke, None; Vogt, Bruno; Hadam,; Lademann, None; Rühl,; Alexiev,; Gruber,

doi:10.1515/nano.bjneah.6.25

Cited by 14 publications

(18 citation statements)

References 115 publications

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“…Nanoparticle detection in cells and tissues are often achieved by employing electron microscopy techniques and confocal microscopy to investigate translocation of NPs in cells [72][73][74]. While these techniques have extensively accomplished identification of NPs, they lack the potential to validate NPs presence in cells or tissue via spectral mapping.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Manaloto

Gowen²,

Leśniak³

et al. 2020

Preprint

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Running title: Cold Atmospheric Plasma enhances silver nanoparticle uptake and cytotoxicity.2 Abstract Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display synergistic cytotoxicity when combined with CAP against cancer cells but there is a paucity of information using AgNP, prompting to investigate the combined effects of CAP using dielectric barrier discharge system (voltage of 75 kV, current is 62.5mA, duty cycle of 7.5kVA and input frequency of 50-60Hz) and 10nm PVA-coated AgNP using U373MG Glioblastoma Multiforme cells. Cytotoxicity in U373MG cells was >100-fold greater when treated with both CAP and PVA-AgNP compared with either therapy alone (IC 50 of 4.30 µg/mL with PVA-AgNP alone compared with 0.07 µg/mL after 25s CAP and 0.01 µg/mL 40s CAP). Combined cytotoxicity was ROS-dependent and was prevented using N-Acetyl Cysteine. A novel darkfield spectral imaging method investigated and quantified AgNP uptake in cells determining significantly enhanced uptake, aggregation and subcellular accumulation following CAP treatment, which was confirmed and quantified using atomic absorption spectroscopy. The results indicate that CAP decreases nanoparticle size, decreases surface charge distribution of AgNP and induces uptake, aggregation and enhanced cytotoxicity in vitro.

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Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Manaloto

Gowen²,

Leśniak³

et al. 2020

Preprint

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“…Moreover, pathological states like inflammation are associated with alterations in several properties of systemic circulation and changes in permeability of blood vessels and immunological modulation. These alterations in pathological situations along with the fact that macrophages are major mediators of nanoparticle biokinetics in vivo alter the distribution of nanoparticles between physiological and pathological states 15–17 . In addition, the cellular biokinetics is influenced by the geometry of nanoparticles.…”

Section: Nanoparticles—as Radiopharmaceutical Vectorsmentioning

confidence: 99%

Nanoparticulate formulations of radiopharmaceuticals: Strategy to improve targeting and biodistribution properties

Datta

Ray

2020

Labelled Comp Radiopharmac

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Application of nanotechnology principles in drug delivery has created opportunities for treatment of several diseases. Nanotechnology offers the advantage of overcoming the adverse biopharmaceutics or pharmacokinetic properties of drug molecules, to be determined by the transport properties of the particles themselves. Through the manipulation of size, shape, charge, and type of nanoparticle delivery system, variety of distribution profiles may be obtained. However, there still exists greater need to derive and standardize definitive structure property relationships for the distribution profiles of the delivery system. When applied to radiopharmaceuticals, the delivery systems assume greater significance. For the safety and efficacy of both diagnostics and therapeutic radiopharmaceuticals, selective localization in target tissue is even more important. At the same time, the synthesis and fabrication reactions of radiolabelled nanoparticles need to be completed in much shorter time. Moreover, the extensive understanding of the several interesting optical and magnetic properties of materials in nanoscale provides for achieving multiple objectives in nuclear medicine. This review discusses the various nanoparticle systems, which are applied for radionuclides and analyses the important bottlenecks that are required to be overcome for their more widespread clinical adaptation.

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“…A variety of methods of detection and identification of NPs in different environments have been used and reported in literature. Atomic force microscopy, scanning tunneling microscopy, transmission electron microscopy, confocal laser scanning microscopy, fluorescent spectroscopy, spectrophotometry, photothermal microscopy (PTM), X-ray and mass spectrometry are some of the methods used for detection and characterization (Nedosekin et al 2015;Ostrowski et al 2015;Hendrickson et al 2011;Me'rian et al 2012;Théoreta and Wilkinson 2017). Although, dark-field microscopy faces challenges on account of low sensitivity and limited axial resolution, it has found wide use in the detection, identification, and tracking of various NPs with the assistance of multispectral imaging for spectral identification of NPs (Théoreta and Wilkinson 2017;Lee and Tung 2012;Mustafa et al 2013).…”

Section: Toxicity Of Nanoparticlesmentioning

confidence: 99%

Toxicity of nanoparticles_ challenges and opportunities

Ramanathan

2019

Appl. Microsc.

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Nanomaterials (NMs) find widespread use in different industries that range from agriculture, food, medicine, pharmaceuticals, and electronics to cosmetics. It is the exceptional properties of these materials at the nanoscale, which make them successful as growth promoters, drug carriers, catalysts, filters and fillers, but a price must be paid via the potential toxity of these materials. The harmful effects of nanoparticles (NPs) to environment, human and animal health needs to be investigated and critically examined, to find appropriate solutions and lower the risks involved in the manufacture and use of these exotic materials. The vast number and complex interaction of NM/NPs with different biological systems implies that there is no universal toxicity mechanism or assessment method. The various challenges need to be overcome and a number of research studies have been conducted during the past decade on different NMs to explore the possible mechanisms of uptake, concentrations/dosage and toxicity levels. This review article examines critically the recent reports in this field to summarize and present opportunities for safer design using case studies from published literature.

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Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Abstract: This article is part of the Thematic Series "Biological responses to NPs".

Cited by 14 publications

References 115 publications

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Nanoparticulate formulations of radiopharmaceuticals: Strategy to improve targeting and biodistribution properties

Toxicity of nanoparticles_ challenges and opportunities

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