2020
DOI: 10.1016/j.prevetmed.2019.104704
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Overview of diagnostic tools for Capripox virus infections

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Cited by 22 publications
(27 citation statements)
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“…The immunoperoxidase assay has also been developed for the detection of antibodies against LSDV, SPPV, and GTPV [ 151 ]. Other techniques such as Western Blot, counter–immunoelectrophoresis test (CIE), or agar gel precipitation test have also been described for the detection of anti-capripox antibodies [ 152 ]. The evaluation of the cell-mediated response is carried out by the lymphocyte proliferation is carried through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and by mRNA cytokine expression of which IFN-γ and IL-4 are the most important against Capripoxvirus infection [ 70 , 71 ].…”
Section: Vaccination Against Capripoxvirusesmentioning
confidence: 99%
“…The immunoperoxidase assay has also been developed for the detection of antibodies against LSDV, SPPV, and GTPV [ 151 ]. Other techniques such as Western Blot, counter–immunoelectrophoresis test (CIE), or agar gel precipitation test have also been described for the detection of anti-capripox antibodies [ 152 ]. The evaluation of the cell-mediated response is carried out by the lymphocyte proliferation is carried through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and by mRNA cytokine expression of which IFN-γ and IL-4 are the most important against Capripoxvirus infection [ 70 , 71 ].…”
Section: Vaccination Against Capripoxvirusesmentioning
confidence: 99%
“…Poxvirus infection suspected sheep farms were visited. External clinic examinations and enzyme-linked immunosorbent assay (ELISA) test for sheeppox virus (Dashprakash et al, 2019;Haegeman et al, 2019) (abbexa® Cambirdge-UK) were performed on suspected animals. Seropositive sheep and their lambs, having poxvirus exanthema (around lips, eyelids and ears, and skin of inguinal, perineal and sub-tail regions) (from erythema to scabs) and other poxvirus infection signs, accepted as infected with sheep-poxvirus.…”
Section: Experimental Animalsmentioning
confidence: 99%
“…At that time, it is reported that serological diagnostic techniques are successfully used for detection of antibody created by the organism against sheeppox virus like other virus detection methods (Balinsky et al, 2008;Dashprakash et al, 2019;Haegeman et al, 2019) It is well known that pox disease in general leads to physiological (oxidative) stress. Hereby, it affects metabolism and excretion of animals (Chirase et al, 1991;Machado et al, 2013;Orr et al, 1990;Paksoy, 2015).…”
Section: Introductionmentioning
confidence: 99%
“…Next to virus isolation in primary cell cultures [12,13] or continuous cell lines [14], and virus detection via antigen capture ELISAs [15,16] or electron microscopy [17,18], detection of CaPV viral genomes [19] is an important tool for the confirmation of CaPV cases and outbreaks. Here, different PCR detection systems have been developed over the years, ranging from conventional gel-based assays combined with agarose gel electrophoresis [20] or with restriction fragment length polymorphism (RFLP) analyses [21] through loop-mediated isothermal amplification (LAMP) assays [22,23] and high-resolution melting curve analyses [24] to real-time quantitative PCR (qPCR) assays using intercalating dyes [20] or fluorescence-labelled probes [6,25,26].…”
Section: Introductionmentioning
confidence: 99%
“…Conventional PCR assays enable qualitative analysis by agarose gel electrophoresis after PCR amplification [27]. In contrast, real-time PCR assays utilizing intercalating dyes or fluorescence-labelled probes in combination with quenchers allow real-time monitoring of the amplification process [19]. In addition, quantitative PCRs are faster, more specific, and in some cases even more sensitive compared to conventional PCRs [27,28].…”
Section: Introductionmentioning
confidence: 99%