Overview of Electrophysiological Techniques

Wickenden, Alan D.

doi:10.1002/0471141755.ph1101s64

Cited by 18 publications

(19 citation statements)

References 68 publications

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“…43,[63][64][65][66][67][68] Among those applications, this section of the review focuses on the interfaces between NW-based nanoelectronics and living cells for direct recording of cellular electrophysiological signals, which is important for understanding numerous physiological processes, including nerve conduction, muscle contraction, hormone secretion, etc. [69][70][71] We introduce (1) the operation schematic of a semiconductor NW-based FET, (2) extracellular electrophysiological studies of cultured cells, tissues, and organs at the in vivo level with the use of on-chip and/or flexible Reprinted from Cohen et al 73 (D) Schematic of the free-standing macroporous nanoelectronic scaffold with nanowire FET arrays (red dots). Reprinted by permission from Springer Customer Service Centre GmbH: Springer Nature, Nature Nanotechnology, Dai et al, 63 nanoelectronics with planar NW structure, and (3) intracellular electrophysiological studies using 3D nanoprobes with advanced NW structure at the cellular and subcellular levels.…”

Section: Semiconductor Nanowires With Living Cells For Electrophysiologymentioning

confidence: 99%

“…Reprinted with permission from (J, i; and K) Tian et al 11 (copyright 2010 AAAS), (J, ii) Xu et al 75 (copyright 2014 American Chemical Society), and (J, iii) Zhao et al 76 (LFP), and this LFP can be detected by positioned microelectrode arrays or conventional FET transducers with microscale device dimensions. 71 Compared with those recording methods, NW-based FETs have substantial advantages in achieving nanoscale interfaces between recording transducers and biological systems. Together with signal amplification, NW-based FETs are able to achieve high-quality singleunit extracellular electrophysiological signal recording or large-scale multiplexed recording in complex cellular networks by NW-based FET arrays with high spatiotemporal resolution.…”

Section: Extracellular Electrophysiological Study Of Cultured Cells mentioning

confidence: 99%

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Recent Advances in Nanowire-Biosystem Interfaces: From Chemical Conversion, Energy Production to Electrophysiology

Zhao

Owusu

et al. 2018

Chem

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The nano-bio interface generated by integrating semiconductor nanomaterials with living cells could serve as a platform for facilitating energy and signal transfer between non-living materials and living systems for applications in energy and the life sciences. This review presents recent advances in one-dimensional nanomaterial-biosystem interfaces and applications from chemical conversion and energy production to electrophysiology. First, we introduce representative types of nanowire-biosystem interfaces and their design principles. Second, we present nanomaterial-bacteria hybrids for solar-to-chemical CO 2 reduction. Third, we introduce nano-bio hybrid electrodes for energy production, especially for biofuel cells. Fourth, we present semiconductor nanowire-embedded nanoelectronics interfaced with living cells and tissue for electrophysiological signal recordings. Last, we provide a brief summary of the progress on energy and signal transfer at the nano-bio interface, as well as our perspectives on the challenges and future directions in this interesting field.

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Section: Semiconductor Nanowires With Living Cells For Electrophysiologymentioning

confidence: 99%

Section: Extracellular Electrophysiological Study Of Cultured Cells mentioning

confidence: 99%

Recent Advances in Nanowire-Biosystem Interfaces: From Chemical Conversion, Energy Production to Electrophysiology

Zhao

Owusu

et al. 2018

Chem

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“…The gold standard method for assessing ion channel modulation is patch clamp electrophysiology (Wickenden, ), an approach that has typically been low throughput, time consuming and required considerable expertise. While automated electrophysiology (Liu, ) allows screening of higher numbers of samples for channel modulation, it requires specialized equipment and a high level of expertise.…”

Section: Functional Screens For Ion Channel Modulatorsmentioning

confidence: 99%

“…The most common electrophysiology technique for measuring ion channel function is the whole-cell patch-clamp technique (Goineau, Legrand, & Froget, 2012;Wickenden, 2014), which allows recording of macroscopic ion channel currents from a single cell (Fig. 6).…”

Section: Electrophysiology Assaysmentioning

confidence: 99%

Screening Strategies for the Discovery of Ion Channel Monoclonal Antibodies

et al. 2018

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Ion channels play crucial roles in physiology by modulation of cellular functions that include electrical excitability, secretion, cell migration, and gene transcription. They are an important target class for drug discovery and have historically been targeted using small molecule approaches. A significant opportunity exists to target these channels with antibodies and alternative forms of biologics. Antibodies display high specificity, selectivity, and affinity for their target antigen, thus having the potential to target ion channels very precisely. Nonetheless, isolating antibodies to ion channels is challenging due to the difficulties in expression and purification of ion channels in a format suitable for antibody drug discovery and due to the complexities of screening for function. In this overview, we focus on an array of screening methods, ranging from direct antibody binding screens to complex electrophysiological assays, and describe how these assays can be used to identify functional monoclonal antibodies. We also provide some insights into specific considerations which are required to enable these screens to be used for antibody drug discovery. © 2018 by John Wiley & Sons, Inc.

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“…Current Protocols in Pharmacology with blue light, an optogenetics approach (see UNIT 11.1;Wickenden, 2014;Knopfel and Boyden, 2012). Upon this depolarization, Ca v channels are activated leading to an increase of intracellular Ca 2+ and of the fluorescence.…”

Section: Supplement 75mentioning

confidence: 99%

Functional Studies of Sodium Channels: From Target to Compound Identification

et al. 2016

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Over the last six decades, voltage-gated sodium (Na ) channels have attracted a great deal of scientific and pharmaceutical interest, driving fundamental advances in both biology and technology. The structure and physiological function of these channels have been extensively studied; clinical and genetic data have uncovered their implication in diseases such as epilepsy, arrhythmias, and pain, bringing them into focus as current and future drug targets. While different techniques have been established to record the activity of Na channels, proper determination of their properties still presents serious challenges, depending upon the experimental conditions and the desired subtype of channel to be characterized. The aim of this unit is to review the characteristics of Na channels, their properties, the cells in which they can be studied, and the currently available techniques. Topics covered include the determination of Na -channel biophysical properties as well as the use of toxins to discriminate between subtypes using electrophysiological or optical methods. Perspectives on the development of high-throughput screening assays with their advantages and limitations are also discussed to allow a better understanding of the challenges encountered in voltage-gated sodium channel preclinical drug discovery. © 2016 by John Wiley & Sons, Inc.

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Overview of Electrophysiological Techniques

Cited by 18 publications

References 68 publications

Recent Advances in Nanowire-Biosystem Interfaces: From Chemical Conversion, Energy Production to Electrophysiology

Recent Advances in Nanowire-Biosystem Interfaces: From Chemical Conversion, Energy Production to Electrophysiology

Screening Strategies for the Discovery of Ion Channel Monoclonal Antibodies

Functional Studies of Sodium Channels: From Target to Compound Identification

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