Ovine herpesvirus type 2 infection in captive bison in India

Sood, Richa; Kumar, Manoj; Bhatia, Sandeep; Pateriya, Atul Kumar; Khandia, Rekha; Siddiqui, Arshi; Kulkarni, D. D.; Kumar, Sanjeev; Venkatesha, M. D.

doi:10.1136/vr.e4291

Cited by 6 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of OvHV-2 in South India became evident, when screening of 612 samples from sheep and goats with some respiratory disease, showed that 17 of these were positive [1]. Recently, OvHV-2 was detected in a captive bison, which died of MCF like symptoms at Bannerghatta National Park, Bengaluru [30]. Detailed investigations revealed the occurrences of clinical cases of MCF in cattle in the areas, surrounding the park.…”

Section: Introductionmentioning

confidence: 99%

Cross-sectional study indicates nearly a quarter of sheep population in Karnataka state of India is infected with ovine herpesvirus 2

et al. 2015

Self Cite

View full text Add to dashboard Cite

In a cross-sectional study, prevalence of ovine herpesvirus 2 (family: Herpesviridae, subfamily: Gammaherpesvirinae, genus Macavirus and species: Ovine herpesvirus 2) infection was estimated in sheep population of Karnataka state in India. Based on the three stage cluster sampling method, whole blood samples (356) of sheep were collected from 11 sheep-dense districts of the state. The samples were tested for presence of OvHV-2 genome by recommended hemi-nested polymerase chain reaction (PCR) test. The true prevalence of OvHV-2 infection in sheep population of Karnataka was 24.44 %. Of the 11 district surveyed, highest true prevalence of 42.42 % (CI 25.56-59.29) was found in Raichur followed by Tumkur (39.02 %, CI 24.09-53.96). Inverse distance weighted interpolation of prevalence indicated that OvHV-2 prevalence within a given district is not uniform and there are areas of varied prevalence. The nucleotide sequence of the 422 bp DNA fragment, amplified in PCR, matched 99 % with OvHV-2 reference sequence and other sequences reported from India. Grouping of OvHV-2 sequences obtained from Karnataka with those from Andhra Pradesh, Tamil Nadu and Jammu and Kashmir in the neighbour joining tree indicated a close relationship among the OvHV-2s circulating in India. This is the first study in the country where systematic screening of sheep population of a state for the presence of OvHV-2 infection has been carried out, which indicated a widespread prevalence calling for an urgent need for policy measures to prevent economic losses due to the disease in susceptible cattle and buffalo species.

show abstract

Section: Introductionmentioning

confidence: 99%

Cross-sectional study indicates nearly a quarter of sheep population in Karnataka state of India is infected with ovine herpesvirus 2

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Later the occurrence of sheep associated MCF was reported in cattle (Wani et al 2004). Detection of OvHV-2 associated with respiratory disease or fever in sheep and goat was also reported from Jammu and Kashmir and southern parts of India (Wani et al 2006;Banumathi et al 2008) Very recently SA-MCF was recorded in captive bison from Karnataka state (Sood et al 2012). To the authors knowledge there were no reports of SA-MCF in cattle from southern part of India.…”

Section: Introductionmentioning

confidence: 70%

Detection of OvHV-2 from an outbreak of sheep associated malignant catarrhal fever from crossbred cattle of Southern India

et al. 2014

View full text Add to dashboard Cite

An outbreak of sheep associated malignant catarrhal fever in crossbred cattle in a village of Andhra Pradesh, southern India, affected thirteen adult cows and two calves from a population of forty animals. All the affected animals were died between December and January 2013-14. The clinical and gross postmortem findings were typical of MCF in Indian crossbred cattle. Migrating sheep flocks were suspected source of infection for the cattle. The diagnosis was confirmed by heminested PCR in all the affected cattle and the suspected sheep flock. The PCR provided evidence of ovine herpes virus type 2.

show abstract

“…SA-MCF disease was reported in India in the year 1975 on the basis of histopathological examination (9). The confirmation of SA-MCF disease by OvHV-2 genome detection in sheep and goat (10), captive bison (11), and cattle (12,13) has since then been reported. The prevalence of OvHV-2 in sheep ranged from 24.44% to 85% in various states in India (10,12,14) however, the genetic characterization of OvHV-2 was not reported in any of these studies.…”

Section: Introductionmentioning

confidence: 94%

First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India

et al. 2021

Self Cite

View full text Add to dashboard Cite

Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever (SA-MCF), a highly fatal disease syndrome that predominantly affects susceptible hosts of the order Artiodactyla. In this study, an in-depth clinico-molecular investigation of SA-MCF disease in a morbid 50-days-old cattle calf (Bos taurus indicus) and asymptomatic infection in the in-contact reservoir hosts, sheep (Ovis aries), and goat (Capra hircus) housed on a farm located in the Southern India is reported. An OIE recommended SA-MCF type-specific PCR confirmed the etiological agent as OvHV-2. The genetic characterization and phylogenetic analyses based on the glycoprotein B (gB) gene indicate that three genetic variants of OvHV-2 had infected the animal cluster of this study. As the OvHV-2 infection eventually lead to the death of the cattle calf, and the fact that its gB sequence carried four unique amino acid substitutions (N169S, L594P, I645V, and V730A), an investigation of these substitutions impact on its stability and molecular flexibility was carried out. The mapping of these amino acid substitutions on the three-dimensional structure of gB coupled with supplementary investigations showed that these substitutions conveyed the molecular flexibility to the gB, at the cost of its stability. Future studies would be to investigate whether these gB substitutions have any impact on membrane fusion activity using a virus-free cell-to-cell membrane fusion assay. The study also highlights the importance of adopting stringent biosecurity measures where mixed animal farming is a common practice.

show abstract

Ovine herpesvirus type 2 infection in captive bison in India

Cited by 6 publications

References 3 publications

Cross-sectional study indicates nearly a quarter of sheep population in Karnataka state of India is infected with ovine herpesvirus 2

Cross-sectional study indicates nearly a quarter of sheep population in Karnataka state of India is infected with ovine herpesvirus 2

Detection of OvHV-2 from an outbreak of sheep associated malignant catarrhal fever from crossbred cattle of Southern India

First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India

Contact Info

Product

Resources

About