Ovine microsatellites at the <i>OarCP9, OarCP16, OarCP20, OarCP21, OarCP23</i> and <i>OarCP26</i> loci

Ede, A J; Pierson, C. A.; Crawford, A. M.

doi:10.1111/j.1365-2052.1995.tb02655.x

Cited by 59 publications

(8 citation statements)

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“…We selected 14 polymorphic microsatellite loci that show Mendelian heredity in Norwegian red deer (Haanes et al 2005). These were CSSM03 (Moore et al 1994), OarCP26 (Ede et al 1995), RT5 (Wilson et al 1997), SRCRSP10 (Bhebhe et al 1994), NVHRT73 and NVHRT48 (Røed andMidthjell 1998), McM58 (Hulme et al 1994), OarFCB193 and OarFCB304 (Buchanan and Crawford 1993), BM5004, BM888, BMC1009, BM4208 and BM4107 (Bishop et al 1994). They were amplified on a GeneAmp PCR System 9600 (Applied Biosystems) in 10 ll reaction mixtures with 30-60 ng of genomic template DNA, 2 pmol of each primer, 50 mM KCl, 1.5 mM MgCl 2 , 10 mM Tris-HCl, 0.2 mM dNTP, and 0.5 U of AmpliTaq DNA polymerase (Applied Biosystems).…”

Section: Sampling and Genetic Analysesmentioning

confidence: 99%

Consequences for genetic diversity and population performance of introducing continental red deer into the northern distribution range

et al. 2010

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For several centuries, game management has involved translocations of non-native individuals of many species to reinforce local native populations. However, there are few quantitative studies of potentially negative effects on population viability as expected when taxa with different local adaptations hybridise. The European red deer has been subject to particularly many translocations. Around 1900, a total of 17 red deer of Hungarian (Cervus elaphus hippelaphus) and German (C. e. germanicus) origin were introduced onto the island of Otterøya in Norway where few native red deer (C. e. atlanticus) remained (n * 13). To assess interbreeding, the present stock on Otterøya and the indigenous Norwegian and Hungarian populations were characterised in 14 microsatellite loci and in the control region of mtDNA. An intermediate level of genetic variation in the Otterøya population and the presence of population specific alleles from both the indigenous Norwegian and the Hungarian population demonstrate that the introduced red deer interbred with the native. Even distributions of one indigenous and one non-indigenous mtDNA haplotype in the Otterøya population and two point estimates of admixture indicate similar genetic contributions from the two parental populations into the hybrid stock. Low numbers of migrants identified with Bayesian assignment tests demonstrate low recent gene flow from Otterøya into the Norwegian mainland population. The Otterøya hybrid stock has grown vastly in numbers during recent decades, suggesting a high population viability. We observed that the body mass of red deer on Otterøya was similar or greater than in adjacent indigenous Norwegian stocks, indicating that population performance has not been reduced in the hybrid stock and that gene flow probably has not had any negative effects.

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Section: Sampling and Genetic Analysesmentioning

confidence: 99%

Consequences for genetic diversity and population performance of introducing continental red deer into the northern distribution range

et al. 2010

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“…For nuclear loci, samples were genotyped with 15 microsatellite markers optimized in three multiplex PCRs and two capillary electrophoresis runs [MP1: CelJP15, MM12, OarFCB304, OarFCB193, ETH225, BM1818; MP2: OarFCB5, TGLA53, CelJP38; MP3: CSSM19, RME25, CSSM22, CSSM43, OarCP26, CSPS115 (Buchanan and Crawford 1993;Steffen et al 1993;Bishop et al 1994;Moore et al 1994;Grosse et al 1995;Ede et al 1995;Mommens et al 1994;Coulson et al 1998]. DNA sequencer ABI PRISM 3130 and GENEMAPPER software (Applied Biosystems) were used to determine allele sizes.…”

Section: Subspeciesmentioning

confidence: 99%

Iberian red deer: paraphyletic nature at mtDNAbut nuclear markers support its genetic identity

Carranza

Salinas

Andrés

et al. 2016

Ecology and Evolution

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Red deer populations in the Iberian glacial refugium were the main source for postglacial recolonization and subspecific radiation in north‐western Europe. However, the phylogenetic history of Iberian red deer (Cervus elaphus hispanicus) and its relationships with northern European populations remain uncertain. Here, we study DNA sequences at the mitochondrial control region along with STR markers for over 680 specimens from all the main red deer populations in Spain and other west European areas. Our results from mitochondrial and genomic DNA show contrasting patterns, likely related to the nature of these types of DNA markers and their specific processes of change over time. The results, taken together, bring support to two distinct, cryptic maternal lineages for Iberian red deer that predated the last glacial maximum and that have maintained geographically well differentiated until present. Haplotype relationships show that only one of them contributed to the northern postglacial recolonization. However, allele frequencies of nuclear markers evidenced one main differentiation between Iberian and northern European subspecies although also supported the structure of both matrilines within Iberia. Thus, our findings reveal a paraphyletic nature for Iberian red deer but also its genetic identity and differentiation with respect to northern subspecies. Finally, we suggest that maintaining the singularity of Iberian red deer requires preventing not only restocking practices with red deer specimens belonging to other European populations but also translocations between both Iberian lineages.

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“…Genomic DNA was isolated from muscle tissue (Qiagen, DNeasy KIT) and genotyping performed using the following 14 dinucleotide microsatellite loci: CSSM03 (Moore et al 1994); OarCP26 (Ede et al 1995);RT5 (Wilson et al 1997); SRCRSP10 (Bhebhe et al 1994); NVHRT73 and NVHRT48 (Røed and Midthjell 1998);McM58 (Hulme et al 1994); OarFCB193 and OarFCB304 (Buchanan and Crawford 1993) and BM5004, BM888, BMC1009, BM4208 and BM4107 (Bishop et al 1994). Loci were amplified on a GeneAmp PCR System 9600 (Applied Biosystems, CA, USA) in 10 μL reaction mixtures with 30-60 ng of genomic template DNA, 2 pmol of each primer, 50 mM KCl, 1.5 mM MgCl 2 , 10 mM Tris-HCl, 0.2 mM dNTP and 0.5 U of AmpliTaq DNA polymerase (Applied Biosystems).…”

Section: Sampling and Laboratory Proceduresmentioning

confidence: 99%

Low genetic variation support bottlenecks in Scandinavian red deer

et al. 2011

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Loss of genetic variation from genetic drift during population bottlenecks has been shown for many species. Red deer (Cervus elaphus) may have been exposed to bottlenecks due to founder events during postglacial colonisation in the early Holocene and during known population reductions in the eighteenth and nineteenth centuries. In this study, we assess loss of genetic variation in Scandinavian red deer due to potential bottlenecks by comparing microsatellite (n=14) and mitochondrial DNA variation in the Norwegian and Swedish populations with the Scottish, Lithuanian and Hungarian populations. Bottlenecks are also assessed from the M ratio of populations, heterozygosity excess and from hierarchical Bayesian analyses of their demographic history. Strong genetic drift and differentiation was identified in both Scandinavian populations. Microsatellite variation was lower in both Scandinavian populations compared with the other European populations and mitochondrial DNA variation was especially low in the Swedish population where only one unique haplotype was observed. Loss of microsatellite alleles was demonstrated by low M ratios in all populations except the Hungarian. M ratios' were especially low in the Scandinavian populations, indicating additional or more severe bottlenecks. Heterozygosity excess compared with the expectation from the number of observed microsatellite alleles suggested a recent bottleneck of low severity in the Norwegian population. Hierarchical Bayesian coalescent analyses consistently yielded estimates of a large ancestral and a small current population size in all investigated European populations and suggested the onset of population decline to be between 5,000 and 10,000 years ago, which coincide well with postglacial colonisation.

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Ovine microsatellites at the OarCP9, OarCP16, OarCP20, OarCP21, OarCP23 and OarCP26 loci

Cited by 59 publications

References 0 publications

Consequences for genetic diversity and population performance of introducing continental red deer into the northern distribution range

Consequences for genetic diversity and population performance of introducing continental red deer into the northern distribution range

Iberian red deer: paraphyletic nature at mtDNAbut nuclear markers support its genetic identity

Low genetic variation support bottlenecks in Scandinavian red deer

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