Ovine pericardium as a new material for duraplasty

Abstract

The authors report on their 2 1/2-year clinical experience using a dural substitute, ovine pericardium, stabilized with 0.3% glutaraldehyde, flat freeze-dried, and sterilized with gamma-irradiation. Packaging of the ovine pericardium in double-plastic transparent bags allows simple storage in operating rooms and the opportunity for the surgeon to choose an ideal graft according to its shape, size, and plasticity. The ovine pericardia were examined histologically and by transmission and scanning electron microscopy in their native, freeze-dried, and irradiated forms. The final product is composed solely of pericardium fibrosum interwoven with artificially formed extracellular microcavities that serve as natural pores for the ingrowth of host tissue. The surfaces of the grafts appear smooth on the inner and rough on the outer sides. Sixteen grafts were examined macroscopically at autopsy and four of these were also examined histologically to illustrate their successful healing with no adhesion to the underlying brain. As a new dural substitute, ovine pericardium proved to be superior to bovine and allogeneic pericardia because of its workability, flexibility, and reduced thickness. In a study of 120 grafts, all but one healed without complications.

Detailed evaluation of 2959 allogeneic and xenogeneic dense connective tissue grafts (fascia lata, pericardium, and dura mater) used in the course of 20 years for duraplasty in neurosurgery

“…Xenogeneic grafts (No. 881) were prepared from the bovine endothoracic fascia, and bovine or ovine pericardia [25,[29][30][31]34].…”

“…At the present time, allogeneic fascia lata is collected and processed under aseptic conditions, decontaminated by s.c. cold shock [8], packed in plastic bags and stored at a temperature of -50~ till -80~ Xenogeneic pericardium is stabilized by 0.3% glutaraldehyde, for 14 days freeze-dried packed in doubled plastic bags, sterilized by gamma radiation at a dose of 25 kGy and stored at room temperature [19,20,25,301, In allogeneic pericardium the glutaraldehyde stabilization was omitted, the graft was freeze-dried and sterilized by gamma radiation at the same dose. Allogeneic dura mater is processed by antigen extraction and by immersion to NaOH solution to prevent Creutzfeldt-Jakob disease (CJD) transmission.…”

“…Nevertheless, the grafts prepared varied from 2 • 1 cm to 15 • 7 cm. Since 1988 the grafts are then wrapped separately in double-plastic bags, the outer bag a peel-able sterilization pack [25,30].…”

“…During and after processing the DCTG were until 1987 rolled on a polyamide texture carriers and packed in National Blood Service jars [26,30], later on the grafts were sealed in the double-plastic transparent bags, a Steriking Cover Bags (a peel-sterilization pack) available from Wipak Medical, Nastola, Finland [25,30]. Sterilization by gamma irradiation was performed using the Radiation Sterilization Centre Bioster, Veverskfi Bft~ka, Czech Republic.…”

Peritônio bovino conservado na correção de hérnia ventral em ratos: uma alternativa para tela cirúrgica biológica

“…As telas absorvíveis, sintéticas ou biológicas, apresentam vantagens inerentes à sua característica de absorção pelo organismo. Entre as próteses absorvíveis biológicas mais estudadas e utilizadas estão: a dura-máter humana 5,6 , a membrana amniótica humana 7,8 , o saco herniário humano 9,10 , a fáscia-lata humana 11,12 , matrizes extracelulares de colágeno suíno [13][14][15] e o pericárdio de bovinos 16,17 , eqüinos 18 ou caprinos 19 . O pericárdio bovino é uma prótese biológica de uso corrente mas apresenta limitação na extensão do tecido obtido para implante.…”