Oviposition and Embryotoxicity ofIndigofera suffruticosaon Early Development ofAedes aegypti(Diptera: Culicidae)

Abstract

Aqueous extract of Indigofera suffruticosa leaves obtained by infusion was used to evaluate the oviposition, its effect on development of eggs and larvae, and morphological changes in larvae of Aedes aegypti. The bioassays were carried out with aqueous extract in different concentrations on eggs, larvae, and female mosquitoes, and the morphological changes were observed in midgut of larvae. The extract showed repellent activity on A. aegypti mosquitoes, reducing significantly the egg laying by females with control substrate (343 (185–406)) compared with the treated substrate (88 (13–210)). No eclosion of A. aegypti eggs at different concentrations studied was observed. The controleclodedin 35%. At concentration of 250 μg/mL, 93.3% of larvae remained in the second instar of development and at concentrations of 500, 750, and 1000 μg/mL the inhibitory effect was lower with percentages of 20%, 53.3%, and 46.6%, respectively. Morphological changes like disruption on the peritrophic envelope (PE), discontinued underlying epithelium, increased gut lumen, and segments with hypertrophic aspects were observed in anterior region of medium midgut of larvae of A. aegypti. The results showed repellent activity, specific embryotoxicity, and general growth retardation in A. aegypti by medium containing aqueous extract of I. suffruticosa leaves.

Indigofera suffruticosa Mill (Fabaceae): Hepatic Responses in Mice Bearing Sarcoma 180

“…In phytochemical studies of I. suffruticosa leaves the Indican was identified, isolated and purified (Vieira, 2011). Recently, protective effects on liver tissue, antitumor activity and embryotoxicity on Aedes aegypti have been tested with extract of I. suffruticosa leaves (Lima, 2012;Vieira et al, 2012). In the present study, we have investigated the liver tissue of mice bearing Sarcoma 180 submitted to subchronic treatment with aqueous extract of Indigofera suffruticosa leaves obtained by infusion and maceration.…”

Hepatoprotective Efficacy of Methanolic Extract of Indigofera Suffruticosa (Mill) on Paracetamol-Induced Liver Damage in Mice

“…Pharmacological studies showed aqueous extracts of leaves of I. suffuticosa have anti-inflammatory, antimicrobial and embryotoxic properties, besides being used as alternative anticancer and antitumor therapy (12,(15)(16)(17)(18) . The aqueous leaf extract of I. suffruticosa has been shown to inhibit egg hatching, larval ecdyse and to have detergent effects on oviposition of Aedes aegypti mosquitoes (19) . Mice carrying sarcoma 180 had positive hepatic response to subchronic treatment with methanolic extract of I. suffruticosa leaves (20) .…”

Erythrocyte Osmotic Fragility and Cytotoxic Activity of the Aqueous Extract of Leaves of Indigofera Suffruticosa Mill (Fabaceae)

“…6 It has also been demonstrated that the aqueous extract of leaves of I. suffruticosa inhibits the development of egg hatching, larval ecdysis and has a deterrent effect on oviposition of Aedes aegypti mosquitoes. 10 Mice with sarcoma 180 presented tumor inhibition with low order of toxicity when treated with the extract at the dose of 50 mg.kg -1 (ip) by infusion (64.53 %) and maceration (62.62 %) Table 2. Effects of AELIs on Allium root growth of Allium cepa (n = 5 onions/concentration or group)…”

“…9 The aqueous extract of I. suffruticosa leaves has been shown to inhibit the development of egg hatching, larval ecdysis and the deterrent effect on oviposition of Aedes aegypti mosquitoes. 10 Mice with sarcoma 180 showed liver response after sub-chronic treatment with I. suffruticosa extract. 11 The phytochemical study of three (3) bis-indolic alkaloid fractions; Indigo and Indirubin from I. suffruticosa leaves were identified, isolated and purified.…”

“…11 The phytochemical study of three (3) bis-indolic alkaloid fractions; Indigo and Indirubin from I. suffruticosa leaves were identified, isolated and purified. 10 However, few reports are available on the toxicological properties of I. suffruticosa, especially its genotoxic effects. Due to the anti-inflammatory, anticancer and antitumor properties, liver protective agent and low toxicity level, previously reported, our hypothesis is that the plant has low hemolytic effects and cytotoxicity.…”