Ovopel® and carp pituitary extract for induction of reproduction in Colossoma macropomum females

Colossoma macropomum females can reproduce more than once in the same reproductive period

“…The similar means between the two spawnings for the variables of total oocyte weight, production index, and absolute fecundity indicate that oocyte production was not reduced at the second spawning. The total oocyte weight and production index obtained in both the first and second spawnings were similar to those reported by Souza et al (2018) in a single spawning of C. macropomum. There was a greater total oocyte weight (Galo et al, 2015) and absolute fecundity (Vieira et al, 1999) with females that had greater body weights, but without great changes in production index (ratio between oocyte weight and fish weight), as demonstrated in the study of Galo et al (2015).…”

“…This reinforces the need to improve reproductive protocols using new hormonal inducers (currently, only carp pituitary extract is being used in C. macropomum females) and different hormone doses and/or intervals between doses. It should be emphasized that different hormonal inducers have been successfully evaluated in C. macropomum males (Martins et al, 2017) and females (Souza et al, 2018), but there are no reports on effects on the survival of fish in comparison with the traditional protocol involving carp pituitary extract.…”

Reproductive performance of lambari ( Astyanax altiparanae ) in a seminatural system using different protocols

“…Moreover, the intermediate reproductive performance obtained using 1,000 μg sGnRHa/kg highlights that future approaches testing dopamine inhibitors together with sGnRHa may change the current scenario. Even unusual and much higher than that the doses used for hormonal induction for neotropical (Acuña & Rangel, 2009; Pereira et al., 2016, 2018; Souza et al., 2018, 2020; Viveiros et al., 2013), cyprinids (Podhorec et al., 2016; Podhorec, Socha, Sokolowska‐Mikolajczyk, Policar, Svinger, Drozd, et al., 2012; Podhorec, Socha, Sokolowska‐Mikolajczyk, Policar, Svinger, Gosiewski, et al., 2012) and salmonids fishes (Arabaci et al., 2004), the 1,000 μg sGnRHa/kg treatment resulted in fertility and hatching rates above 90%, indicating that the use of this synthetic treatment is not a problem for this species in this concern.…”

“…Moreover, for South American reophilic tropical species, the action of dopamine inhibitors remains misunderstood due to the controversial results found in the literature. The use of Ovopel (mGnRHa + metoclopramide) for Colossoma macropomum provoked 100% of spawning rate and satisfactory fertilization (71.4%) and hatching rates (56.8%) (Souza et al., 2018). In contrast, using the same association for Leporinus macrocephalus , 100% spawning was obtained but without viable embryos (Pereira et al., 2016).…”

“…However, the efficiency of exogenous hormones for induction of spawning exhibits considerable variation between species and may be species‐specific, for either dose of hormonal treatment, administration method, or degree of sexual maturity at time of treatment (DiMaggio et al., 2014; Zadmajid & Butts, 2018; Zohar & Mylonas, 2001). For Neotropical fishes, the physiological effects of synthetic hormones used to induce spawning remain poorly understood, and they can vary according to the nature of the molecule and the species of fish (Acuña & Rangel, 2009; Carneiro & Mikos, 2008; Felizardo et al., 2012; Ittzés et al., 2014; Paulino et al., 2011; Pereira et al., 2016; Souza et al., 2018; Viveiros et al., 2013). To the best of our knowledge, the use of GnRHa to enhance lambari's reproduction has been reported in only one study.…”