Ovulation as danger signaling event of innate immunity

Oxidative status in granulosa cells of infertile women undergoing IVF

“…OS reflects ROS production as by-products of rapid steroidogenesis in preovulatory GC [Hanukoglu 2006]. The preovulatory follicles are more likely to be susceptible to OS than the earlier stages of follicles [Spanel-Borowski 2010]. Two major sources of ROS production in GC are: i) leaky membranes of the mitochondrial respiratory chain and ii) a GC subtype without cytokeratin (CK) filaments, termed CK negative or (CK-) [Spanel-Borowski 2010].…”

“…The preovulatory follicles are more likely to be susceptible to OS than the earlier stages of follicles [Spanel-Borowski 2010]. Two major sources of ROS production in GC are: i) leaky membranes of the mitochondrial respiratory chain and ii) a GC subtype without cytokeratin (CK) filaments, termed CK negative or (CK-) [Spanel-Borowski 2010]. Degenerating mitochondria in human GC from fresh follicle aspirates are indicative of ROS damage [Vilser et al 2010].…”

The Use of Proteomics in Assisted Reproduction

“…In a recent study by Bianchi et al in the follicular fluid of six women undergoing IVF after ovarian hyperstimulation, 43 unique proteins were identified, functionally classified in 5 groups (inflammation and regulation of acute-phase, complement and coagulation, response to wounding, protein-lipid complex/lipid metabolism and transport, and cytoskeleton organization) and cross-linked to generate a tightly controlled pathway depicting the initial inflammatory-like phase of ovulation, also described in previous studies (87,88), further accentuated by ART superovulation, followed by tissue remodeling that is crucial for the formation of corpus luteum. A1 antithrypsin was differentially expressed in the follicular fluid and sera of women in this cohort (89).…”

Five different phenotypes of endothelial cell cultures from the bovine corpus luteum: Present outcome and role of potential dendritic cells in luteolysis

“…In addition to the microvascular CK + cells, steroidogenic CK + cells represent the second cell type with potential danger sensing function in the CL. The statement relies on the surmise that the preovulatory CK + granulosa cells with an oxLDL-dependent TLR4 regulation continue their life as steroidogenic CK + cells in the developing CL for tissue reconstruction (Serke et al, 2009;Spanel-Borowski 2011b). In the CL stage of secretion with progesterone synthesis at its maximum, the steroidogenic CK + cells respond by getting a new costume without CK filaments (Fig.…”