Ovum pick-up interval in buffalo ( Bubalus bubalis ) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development

Konrad, J. L.; Clérico, G.; Garrido, María José Molina; Taminelli, G.; Yuponi, M.; Yuponi, R.; Crudeli, G.; Sansinena, M.

doi:10.1016/j.anireprosci.2017.06.004

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…In Table 2 Table 3, there was no difference in oocyte recovery rate between once and twice OPU session groups. Percentage of grade 2-4 between once and twice a week OPU session (Konrad et al, 2017) and optimal OPU interval has not been determined due to different feeding system, condition of donor cow, and different OPU system. On the other hand, collection of high quality and high number of oocytes by using OPU will produce high quality blastocyst production.…”

Section: Resultsmentioning

confidence: 99%

“…This result means that, recovery periods for three months was effective for oocyte collection by using OPU compared with two months of recovery period after donor cow. Previous reports have been focused only on the times an OPU session can be repeatedly done from donor cow and the change in the number of collected oocytes for long periods (Konrad et al, 2017). Kruip et al (1994) reported that the collected number of oocytes were similar between two and five months of OPU experiments.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

Kang¹,

Kim²,

Lee³

et al. 2019

J Anim Reprod Biotechnol

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

Kang¹,

Kim²,

Lee³

et al. 2019

J Anim Reprod Biotechnol

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“…The frequency at which OPU sessions are performed could affect the number of follicles available for aspiration (Ferraz et al, 2015). For example, Konrad et al (2017) observed that 14-day intervals between OPU sessions would increase the amount of recovered oocytes (4.5 ± 0.49/animal) compared to 7-day intervals (2.8 ± 0.45/animal). However, in the aspirations performed every 14 days, the availability of good quality oocytes decreases, therefore generally speaking the frequency of aspirations could affect the oocyte recovery rate, but would not modify the final blastocyst production rate (Konrad et al, 2017).…”

Section: Cumulus-oocyte Complex (Cocs) From Live Donorsmentioning

confidence: 99%

In vitro embryo production in buffaloes: from the laboratory to the farm

et al. 2019

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Transvaginal follicular aspiration technique together with in vitro embryo production are the biotechnological alternatives currently available to support genetic improvement breeding programs in buffalo species. However, aspects related to animal management, lack of knowledge of the metabolic needs and biochemical peculiarities of gametes and embryos, as well as the reproductive physiology characteristics have hampered progress in the results. Despite the low availability of good quality oocytes collected after OPU in donors as a physiological characteristic of buffalo species, high rates of oocyte maturation, modest embryo cleavage, blastocyst production and pregnancy rates after transvaginal embryo transfer in recipients could be obtained in buffalo in vitro embryo production programs. The results of implementing an in vitro embryo production program in buffaloes in the northern region of Pará state, Brazil, and results published by other groups demonstrate the feasibility of implementing this biotechnology in the routine of breeding programs. Nevertheless, in order to achieve better and consistent results, it is necessary to deepen the knowledge on the peculiarities of reproductive biology in this specie. Selection of donor animals based on ovarian size and ovarian follicular reserve and on the rate of blastocyst production is presented as an effective alternative to increase the efficiency of the in vitro embryo production technique applied to the buffalo species.

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“…There are significant differences between B. indicus and Bos taurus cows with regard to their reproductive physiology, as B. indicus cattle have a longer prepubertal period, poor estrus expression, reduced diameter of DF and CL, and fewer overt signs of estrus. Previously, many studies have been conducted to optimize the OPU frequency in bovines but most of the studies were either on B. taurus and compared just OW and TW OPU intervals (Gibbons et al, 1994;Lopes et al, 2006;Merton et al, 2003) or on the stage of estrous cycle in cattle (Hendriksen et al, 2004;Petyim et al, 2003) or studied the different OPU intervals in the buffalo (Ferraz et al, 2015;Konrad et al, 2017;Neglia et al, 2011) and their findings are contradictory. The information regarding optimization of OPU intervals, especially in terms of follicular dynamics and gene expression, is lacking in B. indicus cattle in general and in Sahiwal cattle in particular, which needs to be addressed.…”

Section: Introductionmentioning

confidence: 99%

“…The procedure also allows follicles with diameter >2 mm to be visualized and aspirated, thereby resetting the estrous cycle and avoiding dominance of one follicle over its subordinates (Neglia et al, 2011). The outcome of OPU‐based IVEP, in terms of follicle population, oocyte recovery rate, oocyte quality and their development to morula‐blastocyst stages, is influenced by nutrition (Sartori et al, 2017), body condition score (BCS) (Dorice et al, 2019), stage of the estrous cycle (Hendriksen et al, 2004), lactation status (Baruselli et al, 2018), pregnancy status (Ratto et al, 2011), parity (Jin et al, 2016), season of the year in which OPU is carried out (Di Francesco et al, 2012) and OPU interval between consecutive OPU sessions (Konrad et al, 2017). For cattle, strategies to enhance the ovarian follicular population, the percentage of non‐atretic follicles and the COCs quality are essential for improving OPU‐IVEP efficiency.…”

Section: Introductionmentioning

confidence: 99%

Effect of three schemes of ovum pick‐up on the follicular dynamics, gene expression, and in‐vitro developmental competence of oocytes in Sahiwal cattle

Saleem

Yousuf

Ghafoor

et al. 2022

Reprod Domestic Animals

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This study aimed to compare the effect of three schemes of ovum pick-up (OPU) on follicular dynamics, oocytes recovery, oocytes quality, gene expression, nuclear maturation and in-vitro developmental competence of oocytes in Sahiwal cattle.Considering the follicle population, all the cows were divided equally in a 3 × 3 cross over design, and each cow received one of the three treatments: (a) twice weekly (TW; n = 6), (b) once weekly (OW; n = 6) and (c) bi-weekly OPU (BW; n = 6) in three periods, with the first OPU conducted on 4, 7 and 14 days after second dominant follicle puncture (DFP) in the TW, OW and BW OPU interval groups, respectively. The collected cumulus oocytes complexes (COCs) were graded into A, B, C and D grades depending on the number of layers of cumulus cells and homogeneous nature of cytoplasm. Nuclear maturation was assessed by staining the oocytes with Hoechst 33342. The growth rate (mm/day) of dominant follicle (DF) (F1) (0.49 ± 0.21 vs. 0.71 ± 0.26 vs. 1.30 ± 0.27) and first subordinate follicle (F2) (0.85 ± 0.27 vs. 0.71 ± 0.25 vs.1.06 ± 0.29) did not differ (p > .05) among all the three groups. The proportion of animals bearing a corpus luteum (CL) in the BW OPU interval group (53.3%) was significantly higher (p < .05) as compared to TW (13.3%) and OW (18.3%) OPU interval groups. The number of medium-sized follicles and oocyte with grade A and B were significantly higher (p < .05) in the TW (1.16 ± 0.21 and 33.88 ± 0.03) OPU interval group as compared to the OW (0.88 ± 0.22 and 21.54 ± 0.03) and BW (0.55 ± 0.21 and 21.89 ± 0.02) OPU interval groups. However, the number of degenerated oocytes in BW (0.85 ± 0.16) OPU interval group was significantly higher (p < .05) as compared to the TW (0.16 ± 0.15) and OW (0.44 ± 0.16) OPU interval groups. Expression level of growth differentiation factor 9 in TW OPU interval group was significantly higher (p < .05) as compared to the OW and BW OPU interval groups. Likewise, expression level of bone morphogenetic protein 15 (BMP15) in the TW and BW OPU interval groups was significantly higher (p < .05) as compared to the OW OPU interval group.The nuclear maturation rate was significantly higher in the TW (63.64 ± 0.07) and BW (59.26 ± 0.08) OPU groups as compared to OW (51.43 ± 0.06) OPU interval group.However, the cleavage rate (59.30 ± 0.06 vs. 44.29 ± 0.06 vs. 56.67 ± 0.06) did not differ (p > .05) among the three groups. Whereas, the blastocyst rate tended to be higher (p = .06) in the TW (29.07 ± 0.05) and BW (28.33 ± 0.04

show abstract

Ovum pick-up interval in buffalo ( Bubalus bubalis ) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development

Cited by 17 publications

References 35 publications

Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

In vitro embryo production in buffaloes: from the laboratory to the farm

Effect of three schemes of ovum pick‐up on the follicular dynamics, gene expression, and in‐vitro developmental competence of oocytes in Sahiwal cattle

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