Owl monkey gene mapping: the assignment of gene loci for catalase, β-globin gene cluster, HRAS1, insulin, and parathyroid hormone

Abstract

Using somatic cell genetics and Southern blot hybridization, we have mapped five structural genes in the owl monkey, coding for catalase (CAT), the β-globin gene cluster (HBBC), c-Ha-ras 1 (HRAS1), insulin (INS), and parathyroid hormone (PTH). All five loci are mapped to chromosome 19 of karyotype VI (2n = 49,50) of the owl monkey; CAT, HBBC, INS, and PTH can be assigned to chromosome 4 of karyotype V (2n = 46), while CAT and HBBC can be assigned to chromosome 2 of karyotype III (2n = 53). Using in situ hybridization, the CAT gene was precisely mapped on the mid-region and the β-globin gene cluster on the telomeric end of chromosome 2q(K-ΠI). Our results provide significant insight into the evolutionary history of these gene loci. While these loci are separated into at least two major segments in rodents such as the mouse, our results suggest conservation of a single chromosome arm among higher primates.

Genetic analysis of tumorigenesis: a conserved region in the human and Chinese hamster genomes contains genetically identified tumor-suppressor genes.

“…In a recent study of primate genomic evolution, it was shown that CAT, HBBC, HRAS, INS, and PTH are all located on the same chromosome in the owl monkey (27). A similar conservation has been noted in the rat, where HRAS, INS, HBBC, LDHA, CALC, and PTH all have been assigned to chromosome 1 (35).…”

A putative homoeolog of human chromosome 12 in the owl monkey

“…The same hybrid cell lines had been used for other owl mon key gene mapping studies reported previously (Ma, 1983;Ma et al. 1986).…”

Chromosome assignment of the gene loci ETS1 and THY1 in the owl monkey

“…The enzyme-digested DNA was blotted on nitrocellulose or Nylon membranes and hybridized with a hexamcr-labeled or nick-translated probe. Hybridization was performed at 45-65 C in 5 x NaCl sodium citrate, and the filters were washed under stringent conditions as described previously (Ma et al, 1986).…”