OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?

Grosso, Filipa; Carvalho, Karyne Rangel; Quinteira, Sandra; Ramos, Ana Luísa; Carvalho-Assef, Ana Paula D’Alincourt; Asensi, Marise Dutra; Peixe, Luı́sa

doi:10.1093/jac/dkq406

Cited by 61 publications

(47 citation statements)

References 14 publications

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“…Until recently, A. baumannii isolates bearing CHDLs in Spain were related mainly to 15). Interestingly, the emergence and spread of several outbreak or sporadic A. baumannii strains producing OXA-23 related to EC-II and ST2 have been reported around the world (4,11,(16)(17)(18). …”

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confidence: 99%

First Report of an OXA-23 Carbapenemase-Producing Acinetobacter baumannii Clinical Isolate Related to Tn 2006 in Spain

Espinal

Macià

Roca

et al. 2013

Antimicrob Agents Chemother

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A carbapenem-resistant Acinetobacter baumannii clinical isolate belonging to European clone II and sequence type 2 was recovered from a patient in the Son Espases hospital in Mallorca, Spain. Genetic analysis showed the presence of the bla OXA-23 gene in association with the widely disseminated transposon Tn2006. This is the first reported identification of A. baumannii carrying bla OXA-23 in Spain.A cinetobacter baumannii is responsible for hospital outbreaks, and it is considered one of the most important nosocomial pathogens causing bacteremia, pneumonia, and other respiratory and urinary tract infections (1, 2). The abilities to acquire resistance mechanisms and to persist in the environment for long periods of time make this pathogen difficult to eradicate from the clinical setting (3, 4). The resistance of A. baumannii isolates to carbapenems is on the rise mainly in association with a variety of combined mechanisms, among which the production of acquired carbapenem-hydrolyzing OXA-type class D ␤-lactamases (CHDLs), OXA-23 in particular, has been identified worldwide (2).Here we report the first case of OXA-23 in an A. baumannii clinical isolate in Spain and in association with the widely spread Tn2006 transposon.OXA-23 was identified in a clinical isolate from a 59-year-old male patient admitted to the Department of Pneumology at the Son Espases hospital in Mallorca, Spain, in February 2010 because of increased dyspnea, coughing, chills, and purulent secretions during the previous 3 days. The patient was born in Portugal and had lived in Palma de Mallorca since 1998, although he had traveled to Portugal and France during the previous year. The patient had been hospitalized in Lisbon between 7 and 23 December 2009 and then again in another hospital between 26 December 2009 and 8 January 2010. He was a former smoker and had needed multiple hospital admissions because of exacerbations of chronic obstructive pulmonary disease. His regular medication included bronchodilators but no antibiotics. On the second day after admission, A. baumannii was isolated from his sputum and the patient was empirically treated with levofloxacin (500 to 750 mg/ day) but switched to intravenous colistin (2 to 3 MU/8 h) after 6 days because of susceptibility test results. The patient improved and was discharged for specialized home care. After the initial isolation, periodic sputum cultures were taken for several months with no further isolation of the strain.A. baumannii isolate AB 308 was initially identified by MicroScan and confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Bruker Daltonics GmbH, Leipzig, Germany). Antibiotic susceptibilities were determined by MicroScan (Siemens) for most antimicrobial agents, except colistin and tigecycline, which were determined by Etest (AB bioMérieux, Solna, Sweden). These results were interpreted according to CLSI guidelines (5). The AB 308 isolate was resistant to amikacin (Ͼ256 g/ml), ceftazidime (Ͼ128 g/ml), tetracycline, ciprofloxacin, gentamic...

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confidence: 99%

First Report of an OXA-23 Carbapenemase-Producing Acinetobacter baumannii Clinical Isolate Related to Tn 2006 in Spain

Espinal

Macià

Roca

et al. 2013

Antimicrob Agents Chemother

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“…1b). This particular clonal complex, which differs from the international clones I, II, and III, was also described for A. baumannii isolates from Brazil and Spain (26)(27)(28).…”

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confidence: 59%

Outbreak of Extensively Drug-Resistant Acinetobacter baumannii Indigo-Pigmented Strains

Vilacoba

Almuzara²,

Gulone

et al. 2013

J Clin Microbiol

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Acinetobacter baumannii pigmented strains are not common in clinical settings. Here, we report an outbreak caused by indigopigmented A. baumannii strains isolated in an acute care hospital in Argentina from March to September 2012. Pan-PCR assays exposed a unique pattern belonging to the recently described regional CC113 B /CC79 P clonal complex that confirms the relevant relationships among the indigo-pigmented A. baumannii strains. All of them were extensively drug resistant and harbored different genetic elements associated with horizontal genetic transfer, such as the transposon Tn2006, class 2 integrons, AbaR-type islands, IS125, IS26, strA, strB, florR, and the small recombinase ISCR2 associated with the sul2 gene preceded by ISAba1.

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“…According to the MYSTIC Program Brazil 2 it features considerably among the total number of nosocomial bacterial isolates recorded in intensive care units. Moreover, the carbapenemases OXA-23 and OXA-143 continue to be observed in Brazilian hospitals 3 . Recently Zarrilli et al 4 reviewed the phenotypic and genotypic methods for typing of A. baumannii.…”

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confidence: 99%

“…by MLST for population studies 3 . In light of the existing classification, we decided to investigate the potential utility of VNTR analysis using a small, but representative, panel of 36 strains of this collection of OXA-23-producing A. baumannii clinical isolates which were recovered from four different hospitals in Rio de Janeiro between January 2006 and September 2007 and that represented five PFGE types 3,14 .…”

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confidence: 99%

A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil

et al. 2014

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OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?

Abstract: identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring.

Cited by 61 publications

References 14 publications

First Report of an OXA-23 Carbapenemase-Producing Acinetobacter baumannii Clinical Isolate Related to Tn 2006 in Spain

First Report of an OXA-23 Carbapenemase-Producing Acinetobacter baumannii Clinical Isolate Related to Tn 2006 in Spain

Outbreak of Extensively Drug-Resistant Acinetobacter baumannii Indigo-Pigmented Strains

A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil

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