Oxalate oxidase from banana peel for determination of urinary oxalate.

Raghavan, K.G.; Devasagayam, T.P.A.

doi:10.1093/clinchem/31.4.649

Cited by 16 publications

(6 citation statements)

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“…The EMA derivative of banana oxalate oxidase was prepared according to Goldstein [lo]. About 500 mg (wet wt) of banana oxalate oxidase preparation [9] (1.8 U/mg protein) was used for derivatisation.…”

Section: Resultsmentioning

confidence: 99%

“…The recovery of enzyme activity in the EMA derivative ranged between 72 and 80%. In the case of multienzyme derivatisation, a solution containing 300 mg oxalate oxidase [9], 10 mg catalase (Sigma, 2000 U/mg protein) and 100 mg peroxidase (Sigma type I) was used.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, some of these have been overcome to a large extent. We have already developed an inexpensive and rapid procedure for the isolation of oxalate oxidase from banapa peels [9]. Rat, like man, is incapable of metabolizing oxalate and therefore serves as a good model for studies on disorders of oxalate metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…The activities of banana oxalate oxidase was isolated from banana fruit peels (Muss preparations were determined spectrophotometparadiasica cv. French plaintain) as described [9].…”

Section: Methodsmentioning

confidence: 99%

“…We have therefore examined the attractive possibilities of employing enzyme therapy for degrading endogenous oxalate in hyperoxaluric conditions. As a prerequisite, we modified oxalate oxidase, isolated from banana fruit peels [9], by covalently binding it to a polyanionic electrolyte, ethylenemaleic anhydride (EMA), shifted its pH activity curve towards the alkaline range, and thus rendered it functional at the physiological pH of 7.4 of the recipient animal. Here we report the feasibility of reducing the endogenous concentration of oxalate in rats by implanting dialysis membrane capsules containing EMA-oxalate oxidase in their peritoneal cavities.…”

Section: Enzyme Therapymentioning

confidence: 99%

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Degradation of oxalate in rats implanted with immobilized oxalate oxidase

Raghavan

Tarachand

1986

FEBS Letters

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Accumulation of oxalate leads to hyperoxaluria and calcium oxalate nephrolithiasis in man. Since oxalate is a metabolic end product in mammals, the feasibility of its enzymic degradation has been tested in vivo in rats by administering exogenous oxalate oxidase. Oxalate oxidase, isolated from banana fruit peels, in its native form was found to be non-active at the physiological pH of the recipient animal. However, its functional viability in the recipient animal was ensured by its prior binding with ethylenemaleic anhydride, thus shifting its pH activity curve towards the alkaline range. Rats implanted with dialysis membrane capsules containing such immobilized oxalate oxidase in their peritoneal cavities effectively metabolized intraperitoneally injected [Yloxalate as well as its precursor [Wlglyoxalate.The implantation of capsules containing coentrapped multienzyme preparations of oxalate oxidase, catalase and peroxidase led to a further degradation of administered [Wloxalate in rats. Oxalate oxidase Nephrolithiasis

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The activities of banana oxalate oxidase was isolated from banana fruit peels (Muss preparations were determined spectrophotometparadiasica cv. French plaintain) as described [9].…”

Section: Methodsmentioning

confidence: 99%

Section: Enzyme Therapymentioning

confidence: 99%

See 3 more Smart Citations