Oxalate oxidase transgene expression in American chestnut leaves has little effect on photosynthetic or respiratory physiology

Onwumelu, Anuli; Powell, William A.; Newhouse, Andrew E.; Evans, Garrett; Hilles, Gwen; Matthews, Dakota; Coffey, Vernon; Drake, John E.

doi:10.1007/s11056-022-09909-x

Cited by 3 publications

(4 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Toxins like oxalic acid excreted at the mycelial forefront kill the host cells in advance (Lovat and Donnelly 2019;Rigling and Prospero 2018). In accordance, KO of the gene for producing oxalacetate acetylhydrolase (CpOah) reduced much the virulence of the pathogenic fungus (Chen et al 2010; Table 2), similarly as the recombinant expression of Oxo in susceptible chestnut confers resistance in the host and was performed most recently under control a wounding-and pathogen-responsive promoter of gene win3.12 (Carlson et al 2022;Onwumelu et al 2022;Polin et al 2006; see above).…”

Section: Other Fungal Virulence Factorsmentioning

confidence: 88%

“…Higher resistance of American chestnut was obtained using genetic engineering (Newhouse et al 2014;Zhang et al 2013). Specifically, an oxalate oxidase gene (OxO) from wheat was transferred into American chestnut via Agrobacterium-mediated transformation (Carlson et al 2022;Onwumelu et al 2022;Polin et al 2006). Oxalic acid is produced by the fungus C. parasitica and destroys chestnut tree bark tissues, among others by decreasing the intracellular pH and the lignin content (Lovat and Donnelly 2019;Welch et al 2007).…”

Section: Breedingmentioning

confidence: 99%

See 1 more Smart Citation

Applying molecular and genetic methods to trees and their fungal communities

Müller

Kües

Budde

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Forests provide invaluable economic, ecological, and social services. At the same time, they are exposed to several threats, such as fragmentation, changing climatic conditions, or increasingly destructive pests and pathogens. Trees, the inherent species of forests, cannot be viewed as isolated organisms. Manifold (micro)organisms are associated with trees playing a pivotal role in forest ecosystems. Of these organisms, fungi may have the greatest impact on the life of trees. A multitude of molecular and genetic methods are now available to investigate tree species and their associated organisms. Due to their smaller genome sizes compared to tree species, whole genomes of different fungi are routinely compared. Such studies have only recently started in forest tree species. Here, we summarize the application of molecular and genetic methods in forest conservation genetics, tree breeding, and association genetics as well as for the investigation of fungal communities and their interrelated ecological functions. These techniques provide valuable insights into the molecular basis of adaptive traits, the impacts of forest management, and changing environmental conditions on tree species and fungal communities and can enhance tree-breeding cycles due to reduced time for field testing. It becomes clear that there are multifaceted interactions among microbial species as well as between these organisms and trees. We demonstrate the versatility of the different approaches based on case studies on trees and fungi. Key points • Current knowledge of genetic methods applied to forest trees and associated fungi. • Genomic methods are essential in conservation, breeding, management, and research. • Important role of phytobiomes for trees and their ecosystems.

show abstract

Section: Other Fungal Virulence Factorsmentioning

confidence: 88%

Section: Breedingmentioning

confidence: 99%

Applying molecular and genetic methods to trees and their fungal communities

Müller

Kües

Budde

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…V cmax and J max ). We calculated the marginal water cost of carbon acquisition as encapsulated by the g 1 parameter (Medlyn et al ., 2011) as described previously (Drake et al ., 2017a, b; Onwumelu et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

Pushing the envelope: do narrowly and widely distributed Eucalyptus species differ in response to climate warming?

Drake,

Vårhammar,

Aspinwall

et al. 2024

New Phytologist

Self Cite

View full text Add to dashboard Cite

Summary Contemporary climate change will push many tree species into conditions that are outside their current climate envelopes. Using the Eucalyptus genus as a model, we addressed whether species with narrower geographical distributions show constrained ability to cope with warming relative to species with wider distributions, and whether this ability differs among species from tropical and temperate climates. We grew seedlings of widely and narrowly distributed Eucalyptus species from temperate and tropical Australia in a glasshouse under two temperature regimes: the summer temperature at seed origin and +3.5°C. We measured physical traits and leaf‐level gas exchange to assess warming influences on growth rates, allocation patterns, and physiological acclimation capacity. Warming generally stimulated growth, such that higher relative growth rates early in development placed seedlings on a trajectory of greater mass accumulation. The growth enhancement under warming was larger among widely than narrowly distributed species and among temperate rather than tropical provenances. The differential growth enhancement was primarily attributable to leaf area production and adjustments of specific leaf area. Our results suggest that tree species, including those with climate envelopes that will be exceeded by contemporary climate warming, possess capacity to physiologically acclimate but may have varying ability to adjust morphology.

show abstract

“…Plants can further convert hydrogen peroxide into other harmless substances [ 11 ]. OXO detoxifies by oxidizing oxalic acid to produce H 2 O 2 and CO 2 , and plays an important role in plant disease resistance defense, growth, and development [ 12 ]. AGPPase is a major player in the transfer of carbon flow from starch synthase and may play a key role in starch biosynthesis [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Characterization, and Expression Analysis Related to Low-Temperature Stress of the CmGLP Gene Family in Cucumis melo L.

Zhang

Wen

Yuan

et al. 2022

IJMS

View full text Add to dashboard Cite

Germin-like protein (GLP) participates in plant growth and development and plays an important role in plant stress. In the present study, 22 CmGLPs belonging to five classes were identified in the melon genome. Each member of the CmGLPs family contains a typical Cupin_1 domain. We conducted a genome-wide analysis of the melon GLP gene family characterization. CmGLPs were randomly distributed in the melon chromosomes, with the largest number on chromosome 8, having eight family members. Gene duplication events drive the evolution and expansion of the melon GLP gene family. Based on the phylogenetic tree analysis of GLP proteins in melon, rice, Arabidopsis, and cucumber, it was found that the GLP gene families of different species have diverged in evolution. Based on qRT-PCR results, all members of the CmGLP gene family could be expressed in different tissues of melon. Most CmGLP genes were up-regulated after low-temperature stress. The relative expression of CmGLP2-5 increased by 157.13 times at 48 h after low-temperature treatment. This finding suggests that the CmGLP2-5 might play an important role in low-temperature stress in melon. Furthermore, quantitative dual LUC assays indicated that CmMYB23 and CmWRKY33 can bind the promoter fragment of the CmGLP2-5. These results were helpful in understanding the functional succession and evolution of the melon GLP gene family and further revealed the response of CmGLPs to low-temperature stress in melon.

show abstract

Oxalate oxidase transgene expression in American chestnut leaves has little effect on photosynthetic or respiratory physiology

Cited by 3 publications

References 76 publications

Applying molecular and genetic methods to trees and their fungal communities

Applying molecular and genetic methods to trees and their fungal communities

Pushing the envelope: do narrowly and widely distributed Eucalyptus species differ in response to climate warming?

Genome-Wide Identification, Characterization, and Expression Analysis Related to Low-Temperature Stress of the CmGLP Gene Family in Cucumis melo L.

Contact Info

Product

Resources

About