Oxidant-antioxidant levels in patients with bladder tumours

Güneş, Mustafa; Eryılmaz, Recep; Aslan, Rahmi; Taken, Kerem; Demır, Halit; Demir, Canan

doi:10.1080/13685538.2020.1718636

Cited by 12 publications

(18 citation statements)

References 22 publications

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“…Intracellular oxidative stress is a consequence of imbalanced cellular redox status, resulting in oxidative damage to key cellular biomolecules such as DNA, proteins, and lipids. Such alterations in the cell are principally included in the etiopathogenesis of cancer [ 59 , 60 ]. Antioxidant mechanism of action exerted by naturally occurring substances may be important in the prevention of DNA damage, and thus their use is appropriate in the chemopreventive model of NMU-induced mammary carcinogenesis in rats.…”

Section: Discussionmentioning

confidence: 99%

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Kubatka

Kello

Kajo

et al. 2020

IJMS

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Comprehensive scientific data provide evidence that isolated phytochemicals or whole plant foods may beneficially modify carcinogenesis. The aim of this study was to evaluate the oncostatic activities of Rhus coriaria L. (sumac) using animal models (rat and mouse), and cell lines of breast carcinoma. R. coriaria (as a powder) was administered through the diet at two concentrations (low dose: 0.1% (w/w) and high dose: 1 % (w/w)) for the duration of the experiment in a syngeneic 4T1 mouse and chemically-induced rat mammary carcinoma models. After autopsy, histopathological and molecular analyses of tumor samples in rodents were performed. Moreover, in vitro analyses using MCF-7 and MDA-MB-231 cells were conducted. The dominant metabolites present in tested R. coriaria methanolic extract were glycosides of gallic acid (possible gallotannins). In the mouse model, R. coriaria at a higher dose (1%) significantly decreased tumor volume by 27% when compared to controls. In addition, treated tumors showed significant dose-dependent decrease in mitotic activity index by 36.5% and 51% in comparison with the control group. In the chemoprevention study using rats, R. coriaria at a higher dose significantly reduced the tumor incidence by 20% and in lower dose non-significantly reduced tumor frequency by 29% when compared to controls. Evaluations of the mechanism of oncostatic action using valid clinical markers demonstrated several positive alterations in rat tumor cells after the treatment with R. coriaria. In this regard, histopathological analysis of treated tumor specimens showed robust dose-dependent decrease in the ratio of high-/low-grade carcinomas by 66% and 73% compared to controls. In treated rat carcinomas, we found significant caspase-3, Bax, and Bax/Bcl-2 expression increases; on the other side, a significant down-regulation of Bcl-2, Ki67, CD24, ALDH1, and EpCam expressions and MDA levels. When compared to control specimens, evaluation of epigenetic alterations in rat tumor cells in vivo showed significant dose-dependent decrease in lysine methylation status of H3K4m3 and H3K9m3 and dose-dependent increase in lysine acetylation in H4K16ac levels (H4K20m3 was not changed) in treated groups. However, only in lower dose of sumac were significant decreases in the expression of oncogenic miR210 and increase of tumor-suppressive miR145 (miR21, miR22, and miR155 were not changed) observed. Finally, only in lower sumac dose, significant decreases in methylation status of three out of five gene promoters–ATM, PTEN, and TIMP3 (PITX2 and RASSF1 promoters were not changed). In vitro evaluations using methanolic extract of R. coriaria showed significant anticancer efficacy in MCF-7 and MDA-MB-231 cells (using Resazurin, cell cycle, annexin V/PI, caspase-3/7, Bcl-2, PARP, and mitochondrial membrane potential analyses). In conclusion, sumac demonstrated significant oncostatic activities in rodent models of breast carcinoma that were validated by mechanistic studies in vivo and in vitro.

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Section: Discussionmentioning

confidence: 99%

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Kubatka

Kello

Kajo

et al. 2020

IJMS

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“…These radicals also cause DNA damage. However, antioxidants protect the cell against this DNA damage and free radicals 34,35,36,37 .…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…It has been determined that MDA levels increase in diseases such as bladder, prostate and epilepsy. It been found that total oxidant status, which is the serum oxidative stress level, is increased in ischemic/hemorrhagic stroke 35,41,42,43 . In another study, it has been reported that plasma MDA level increased in Parkinson's patients 40 .…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Investigation of Oxidative Stress Level and Antioxidant Enzyme Activities in Operated and Nonoperated Patients with Spontaneous Intracerebral Hematoma

Akyol

Demir

Görken

2022

J Neurol Surg A Cent Eur Neurosurg

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Background: Complex underlying mechanisms consisting of cytotoxic, excitotoxic, and inflammatory effects of intraparenchymal hemorrhage (ICH) are responsible for the highly detrimental effects on brain tissue. Oxidative stress also plays a significant role in brain damage after ICH; however, it is less important than other factors. In this study, we aimed to evaluate the oxidative stress parameters malondialdehyde (MDA) and the antioxidant reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities in operated and non-operated patients with spontaneous intracranial hematoma. Methods: One hunderd patients with spontaneous intracranial hemorrhage and 100 healthy control subjects were included in this study. Within the indication, 50 of the 100 patients underwent decompressive surgery. MDA, GSH, SOD, and catalase activities were measured in the serum obtained from the patients. Results: SOD and CAT levels were lower in the non-operated group than in the operated and control groups. GSH was not different between the operated and non-operated groups, but it was lower in the control group. However, MDA was higher in those who did not undergo surgery than in the other groups. Conclusions: In our study, MDA, an indicator of oxidative stress, was found to be lower in the blood, and CAT and SOD activities were found to be higher in ICH patients who underwent decompression than in those who did not. This is the first study to present the correlations of MDA, SOD, CAT, and GSH in operated and non-operated patients with spontaneous intracranial hematoma.

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“…Oxidative stress can cause cancer formation. In such a situation, antioxidative activities are reduced (1,2) . Catalase (CAT, H 2 O 2 : oxidoreductase, EC 1.…”

Section: Introductionmentioning

confidence: 99%

Effect of radiotherapy on erythrocyte catalase, and carbonic anhydrase activities, serum levels of some trace elements and heavy metals (Zn, Cu, Pb, Cd, Mn, Fe, Mg and Co) in cancer patients

et al. 2022

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Background:We tried to reveal the relationship between the levels of some important minerals, rare elements and heavy metals by measuring serum cobalt (Co), lead (Pb), zinc (Zn), iron (Fe), copper (Cu), cadmium (Cd), magnesium (Mg), manganese (Mn), catalase (CAT) and carbonic anhydrase (CA) levels in head and neck, CNS, esophagus, stomach and breast cancers receiving radiotherapy. Materials and Methods: Serum Cu, Fe, Pb, Zn, Co, Mg, Mn and Cd levels were measured using atomic absorption spectrophotometry. Carbonic anhydrase activity (CA), CO 2 hydration measurements were made using bromothymol blue method as indicator. Results: Catalase activity, carbonic anhydrase activity and serum copper, lead, zinc, iron, cobalt, cadmium, manganese, magnesium measurements for baseline and post-radiotherapy values differ greatly in cancer patients compared to healthy subjects (p<0.05). Conclusion: As a result, these findings have a significant impact on the pathophysiology of cancer. It can be argued that this paper shows a preliminary study for examining the effect of radiotherapy treatment on the activity of erythrocyte catalase, carbonic anhydrase and serum levels of cobalt, lead (Pb), zinc, iron, copper, cadmium, magnesium and manganese in the head and neck, CNS, stomach, esophagus and breast cancers receiving radiotherapy treatment.

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Oxidant-antioxidant levels in patients with bladder tumours

Cited by 12 publications

References 22 publications

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Investigation of Oxidative Stress Level and Antioxidant Enzyme Activities in Operated and Nonoperated Patients with Spontaneous Intracerebral Hematoma

Effect of radiotherapy on erythrocyte catalase, and carbonic anhydrase activities, serum levels of some trace elements and heavy metals (Zn, Cu, Pb, Cd, Mn, Fe, Mg and Co) in cancer patients

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