Oxidant/antioxidant status, paraoxonase activity, and lipid profile in plasma of ovariectomized rats under the influence of estrogen, estrogen combined with progesterone, and genistein

Abstract

IntroductionThe aim of this study was to investigate whether estradiol (E2), E2 combined with progesterone (Prog) (E2/Prog), and genistein (Gen) treatment had antioxidative and anti-hyperlipidemic effects in the plasma of ovariectomized (OVX) rats.Materials and methodsAdult female Sprague–Dawley rats were divided into five groups. Rats in all groups, except for those in a sham group, underwent bilateral ovariectomy under general anesthesia. The groups were as follows: sham group; control OVX group; group treated with estrogen (0.014 mg/kg 17-β E2); group treated with a combination of E2 and Prog (0.014 mg/kg 17-β E2 plus 0.028 mg/kg drospirenone), and group treated with Gen (10 mg/kg/day). Plasma of rats of each treatment group was analyzed to determine the total antioxidant status, total oxidant status, paraoxonase activity, lipid profile, high-density lipoprotein (HDL-chol), low-density lipoprotein (LDL-chol), total cholesterol (Total-C), triacylglycerols, lipoprotein (a), and oxidative stress index.ResultsPlasma Total-C levels and body weight increased in all the OVX groups compared with the sham group (P<0.005). The group treated with E2 had significantly elevated total oxidant status, oxidative stress index, LDL-chol, and Total-C compared with the control group (P<0.005). Gen treatment might lead to lower LDL-chol and Total-C levels compared with E2 treatment.ConclusionsGen treatment might be preferred to E2 treatment for treatment of menopausal symptoms in patients at risk for cardiovascular diseases. However, considering the small sample size of this study, larger studies are needed in this area.

Antioxidant Effects of Allium cepa and Cinnamon on Sex Hormones and Serum Antioxidant Capacity in Female Rats Exposed to Power Frequency Electric and Magnetic Fields

“…Also, production of estrogen and testosterone depend on high LH secretion. It has been indicated that estrogen has significant antioxidant effects at high concentrations (21)(22)(23)(24). Levels of LH and FSH were reduced significantly in rats after six weeks of exposure to ELF-EMF of 50 Hz and 25 μT.…”

Effects of Dietary Flavonoids on Reverse Cholesterol Transport, HDL Metabolism, and HDL function

“…Estrogen has been shown to enhance PON1 activity via the ER in vitro, but without altering PON1 mRNA levels or protein concentrations (132). Daily oral administration of genistein (10 mg/kg body weight) for 8 wk did not alter HDL cholesterol or serum paraoxonase activity in ovariectomized SpragueDawley rats (133). In contrast, in female Sprague-Dawley rats with collagen-induced arthritis, daily oral administration of genistein (20 mg/kg body weight) or daidzein (20 mg/kg body weight) for 50 d significantly improved serum PON1 paraoxonase and arylesterase activities, but did not alter HDL cholesterol (134).…”

Estradiol deficiency is a risk factor for idiopathic benign paroxysmal positional vertigo in postmenopausal female patients

“…The OVX 1 E2 group were given a 17b-E2 (0.25 mL/kg/d, 10 lg/0.1 mL) supplement 16 1 month after the bilateral OVX. The OVX 1 P group were given a progesterone (0.3 mL/kg/d, 10 mg/ 1 mL) supplement 17 1 month after bilateral OVX. The OVX 1 E2 1 P group were given E2 and P supplements 1 month after the bilateral OVX.…”