Oxidation of Elemental Sulfur to Sulfite by
            <i>Thiobacillus thiooxidans</i>
            Cells

Suzuki, Isamu; Chan, Cheryl; Takeuchi, T. L.

doi:10.1128/aem.58.11.3767-3769.1992

Cited by 48 publications

(23 citation statements)

References 8 publications

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“…The models generated here provide new insights into the likely pathways involved in A. thiooxidans sulfur metabolism, closing some of the gaps in the current understanding. Specifically, our results identify internal cell S 0 generation, storage and use, as well as the importance and rapid conversion of SO 2− 3 in these models, both confirming the speculated importance of these two S compounds (Suzuki et al, 1992) and explaining why they have not previously been definitively confirmed by solution chemical characterization alone.…”

Section: Integrated Gene Expression and Sulfur Chemistry A Thiooxidasupporting

confidence: 68%

“…Based on the published studies to date, the first step in microbial S 0 metabolism is thought to be a relatively linear pathway beginning with oxidation to SO 2− 3 , followed by further oxidation to SO 2− 4 (Suzuki et al, 1992;Rohwerder and Sand, 2003). However, here the model developed through combined sulfur chemical and gene expression analyses indicates that S 2 O 2− 3 oxidation/disproportionation reactions are occurring as formation of significant amounts of Other SOI (i.e., indicating the presence of polythionates) and small amounts of S 2 O 2− 3 are observed (Figure 4A).…”

Section: Integrated Gene Expression and Sulfur Chemistry A Thiooxidamentioning

confidence: 99%

See 1 more Smart Citation

New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses

et al. 2020

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Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S 0 and S 2 O 2− 3 metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans S 2 O 2− 3 metabolism model involves the conversion of S 2 O 2− 3 to SO 2− 4 , S 0 and S 4 O 2− 6 , mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase (TetH), sulfide quinone reductase (Sqr), and heterodisulfate reductase (Hdr) proteins. These same proteins, with the addition of rhodanese (Rhd), were identified to convert S 0 to SO 2− 3 , S 2 O 2− 3 and polythionates in the A. thiooxidans S 0 metabolism model. Our combined results shed light onto the important role specifically of TetH in S 2 O 2− 3 metabolism. Also, we show that activity of Hdr proteins rather than Sdo are likely associated with S 0 oxidation. Finally, our data suggest that formation of intracellular S 2 O 2− 3 is a critical step in S 0 metabolism, and that recycling of internally generated SO 2− 3 occurs, through comproportionating reactions that result in S 2 O 2− 3 . Electron microscopy and spectroscopy confirmed intracellular production and storage of S 0 during growth on both S 0 and S 2 O 2− 3 substrates.

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Section: Integrated Gene Expression and Sulfur Chemistry A Thiooxidasupporting

confidence: 68%

Section: Integrated Gene Expression and Sulfur Chemistry A Thiooxidamentioning

confidence: 99%

New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses

et al. 2020

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“…Recently. however, Suzuki and Takeuchi [73] confirmed the accumulation of sulfite in ceils of 7~ thiooxidans in which tile sulfite-oxidizing enzyme system was inhibited with 2-n-heptyl-4-hydroxyquinoline N-oxide. Thus, in 7~ thiooxidans, at least, elemental sulfur is oxidized according to the fl)llowing stoichiometry [73]:…”

Section: Sulfur Oxidationmentioning

confidence: 94%

“…Given that 7: fermoxidans possesses the same sulfur-oxidizing enzyme systems [6], it is assumed that this bacterium utilizes the same pathway for the oxidation of elemental sulfur [73].…”

Section: S ° T O~ + T-1~o -- Hzso~ -~()~ ~ 1t:so4mentioning

confidence: 99%

The chemolithotrophic bacteriumThiobacillus ferrooxidans

Leduc

Ferroni

1994

FEMS Microbiology Reviews

153

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The iron‐oxidizing bacterium ThiobaciUus ferrooxidans is the most important microorganism in mineral leaching. It plays the dominant role in bioextractive processes because of its ability to oxidize both iron and reduced sulfur compounds. T. ferrooxidans is also an important microorganism in acid rock/mine drainage, a serious environmental problem. In this article, the current status of this bacterium is described with particular emphasis on the biomining industry.

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“…The chemical reactions occurring in a single cell for each type of microbial metabolism (see Table 1) are modelled by calculating the per cell rate of the gas production/consumption (see Table 2 (Suzuki et al, 1990;Suzuki et al, 1992) Carboxydotrophs…”

Section: = −mentioning

confidence: 99%

Swansong biospheres II: the final signs of life on terrestrial planets near the end of their habitable lifetimes

O’Malley-James

Cockell

Greaves

et al. 2014

International Journal of Astrobiology

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Oxidation of Elemental Sulfur to Sulfite by Thiobacillus thiooxidans Cells

Abstract: ThiobaciUus thiooxidans cells oxidized elemental sulfur to sulfite, with 1 mol of 02 consumption per mol of sulfur oxidized to sulfite, when the oxidation of sulfite was inhibited with 2-n-heptyl-4-hydroxyquinoline N-oxide.

Cited by 48 publications

References 8 publications

New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses

New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses

The chemolithotrophic bacteriumThiobacillus ferrooxidans

Swansong biospheres II: the final signs of life on terrestrial planets near the end of their habitable lifetimes

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