Oxidation of Glucose to Gluconic Acid by Glucose Oxidase in a Membrane Bioreactor

Tomotani, Ester Junko; Neves, Luiz Carlos Martins das; Vítolo, Michele

doi:10.1385/abab:121:1-3:0149

Cited by 30 publications

(26 citation statements)

References 17 publications

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“…It was previously established that a concentration of hydrogen peroxide above 1.3 mM (0.0442 mg/mL) causes a noncompetitive inhibitory effect on the activity of GO (Tomotani et al, 2005). A complicating factor is that hydrogen peroxide is an obligatory by-product formed when glucose is oxidized to gluconic acid by GO.…”

Section: Resultsmentioning

confidence: 99%

“…Each sample was immersed in a boiling water bath in order to stop the reaction. Measurement of H 2 O 2 formed during the reaction was made according to Tomotani et al (2005).…”

Section: Glucose Oxidasementioning

confidence: 99%

“…In order to stop the reaction, eppendorf tubes containing the samples were immersed in a boiling water bath. The residual amount of H 2 O 2 was determined at 240 nm according to Tomotani et al (2005).…”

Section: Effect Of Catalase On Residual Hydrogen Peroxide Determinationmentioning

confidence: 99%

“…Immediately after withdrawal, each sample was immersed in a boiling water bath for 5 min. Residual H 2 O 2 and consumption of TRS were determined according to Arruda, Vitolo (1996) and Tomotani et al (2005), respectively.…”

Section: Oxidation Of Glucose In a Discontinuous Process (Batch)mentioning

confidence: 99%

“…The standard-linear equation was y = 1.66x + 4.24x10 -3 (r = 0.99998), where y and x are the absorbance and the amount of H 2 O 2 (mg), respectively. Concentration of the standard solution used to obtain the standard plot was varied between 0.0608 and 0.608 mg of H 2 O 2 /mL (Tomotani et al, 2005).…”

mentioning

confidence: 99%

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Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

Silva

Tomotani

Vítolo

2011

Braz. J. Pharm. Sci.

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Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).
Neste trabalho estudou-se a conversão da sacarose em frutose e ácido glicônico, usando as enzimas invertase, glicose oxidase e catalase, através do emprego de processo descontínuo (com adição sequencial ou simultânea das enzimas) e contínuo (adição simultânea das enzimas em reator com membrana acoplado à membrana de ultrafiltração de 100 kDa). Os parâmetros variados foram: a concentração das enzimas, a concentração inicial dos substratos (sacarose e glicose), o pH, a temperatura e a vazão específica de alimentação (processo contínuo). Obteve-se rendimento de 100%, quando a conversão foi conduzida por processo descontínuo em pH 4,5 e a 37 ºC com adição seqüencial das enzimas invertase (14,3 U), glicose oxidase (10.000 U) e catalase (59.000 U)

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Section: Resultsmentioning

confidence: 99%

“…Each sample was immersed in a boiling water bath in order to stop the reaction. Measurement of H 2 O 2 formed during the reaction was made according to Tomotani et al (2005).…”

Section: Glucose Oxidasementioning

confidence: 99%

Section: Effect Of Catalase On Residual Hydrogen Peroxide Determinationmentioning

confidence: 99%

Section: Oxidation Of Glucose In a Discontinuous Process (Batch)mentioning

confidence: 99%

mentioning

confidence: 99%

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Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

Silva

Tomotani

Vítolo

2011

Braz. J. Pharm. Sci.

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Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Thomas

Ohde

Matthes

et al. 2020

Biotech & Bioengineering

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The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient aeration can be overcome or improved. Investigating the mass transport performance in a model protein solution, the microbubble aeration results in higher k L a values related to the applied airstream in comparison with macrobubble aeration. Comparing the aerators at identical k L a of 160 and 60 1/h, the microbubble aeration is resulting in 25 and 44 times enhanced gas utility compared with aeration with macrobubbles. To prove the feasibility of microbubbles in biocatalysis, the productivity of a glucose oxidase catalyzed biotransformation is compared with macrobubble aeration as well as the gas-saving potential. In contrast to the expectation that the same productivities are achieved at identically applied k L a, microbubble aeration increased the gluconic acid productivity by 32% and resulted in 41.6 times higher oxygen utilization. The observed advantages of microbubble aeration are based on the large volume-specific interfacial area combined with a prolonged residence time, which results in a high mass transfer performance, less enzyme deactivation by foam formation, and reduced gas consumption. This makes microbubble aerators favorable for application in biocatalysis.

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Modeling and tuning of glucose oxidase‐catalase system incorporating dissolved oxygen concentration

Ruales‐Salcedo

Prado-Rúbio

Fontalvo

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND Gluconic acid production by a glucose oxidase (GOx) and catalase (CAT) system has been proposed. However, the bioprocess optimal design and operation are limited by the lack of kinetic models of GOx involving oxygen as substrate. Herein, the GOx‐CAT system is modeled considering a continuous oxygen supply at a bioreactor lab scale. Initially, experiments and modeling for parameter estimations of KLa and the individual enzymes were conducted. Then, experiments and modeling for the GOx‐CAT system were performed for final model tuning. Additionally, the model quality was evaluated, allowing for a deeper understanding of the system phenomenology. RESULTS From the oxygen transport model tuning, a highly accurate KLa estimation was obtained (R2>0.98 and confidence interval <2%). The highest oxygen transport rate was obtained for the combined system Buffer‐antifoam‐GOx‐CAT that was 2.5 times larger than that obtained for DI water. Kinetic models for the individual enzymes were very accurate and the parameters were fully identifiable. For the integrated system, the gluconic acid evolution was properly predicted and there was a loss of predictive power for the oxygen model. Parameter identifiability analysis showed that kcatCAT and KMHPCAT interpretability was compromised. However, the sensitivity analysis indicated that the model must not be simplified. CONCLUSION Herein its has been demonstrated that KLa is a complex parameter to be estimated for multi‐enzymatic systems due to its dependency on medium composition, particularly with GOx. Nonetheless, based on the acceptable model predictive power, the calculated parameters could be used for studies on the process design phase. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Oxidation of Glucose to Gluconic Acid by Glucose Oxidase in a Membrane Bioreactor

Cited by 30 publications

References 17 publications

Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Modeling and tuning of glucose oxidase‐catalase system incorporating dissolved oxygen concentration

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