Oxidation of Mitochondrial Proteins and DNA Following Administration of Ethanol

Wieland, P.; Lauterburg, Bernhard H.

doi:10.1006/bbrc.1995.2202

Cited by 89 publications

(34 citation statements)

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“…It is worth noting that increased mitochondrial production of reactive oxygen species (23), oxidation of mitochondrial proteins (24), depressed oxidative phosphorylation activity (25), and disrupted fatty acid metabolism (26) occur in the liver following consumption of alcohol. One objective of our study was to evaluate and compare the possible role of ethanol intake on mtDNA content alteration in HNSC and control saliva.…”

Section: Discussionmentioning

confidence: 99%

Increased Mitochondrial DNA Content in Saliva Associated with Head and Neck Cancer

Jiang

Masayesva

Zahurak

et al. 2005

Clinical Cancer Research

148

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Alterations of the mitochondrial DNA (mtDNA) have been described in human tumors and in other tissues in association with smoking exposure.We did quantitative PCR of cytochrome c oxidase I (Cox I) and cytochrome c oxidase II (Cox II) genes on oral rinse samples obtained from 94 patients with primary head and neck squamous cell carcinoma (HNSC) and a control group of 656 subjects. Mitochondrial DNA/nuclear DNA in saliva from HNSC patients and controls in relationship to smoking exposure, ethanol intake, and tumor stage were examined. Mean levels of Cox I and Cox II in saliva samples were significantly higher in HNSC patients: Cox I, 0.076 [95% confidence interval (95% CI), 0.06-0.09] and Cox II, 0.055 (95% CI, 0.04-0.07) in comparison with controls Cox I, 0.054 (95% CI, 0.05-0.06), P < 0.0001 and Cox II, 0.046 (95% CI, 0.04-0.05), P = 0.003 (t test). MtDNA levels were elevated in primary tumors when compared with matched, pretreatment saliva and significant correlation was noted (Cox I, r = 0.30, P = 0.005 and Cox II, r = 0.33, P = 0.002, respectively, Pearson's correlation). On univariate analysis, smoking, age, HNSC diagnosis, and advanced stage of HNSC were associated with higher level of mtDNA content in saliva. Multivariate analysis showed a significant and independent association of HNSC diagnosis, age, and smoking with increasing mtDNA/nuclear DNA for Cox I and Cox II. mtDNA content alteration is associated with HNSC independently of age and smoking exposure, can be detected in saliva, and may be due to elevation in mtDNA content in primary HNSC.

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Section: Discussionmentioning

confidence: 99%

Increased Mitochondrial DNA Content in Saliva Associated with Head and Neck Cancer

Jiang

Masayesva

Zahurak

et al. 2005

Clinical Cancer Research

148

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“…1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 11 On the other hand, when ethanol was administered in much higher quantities many harmful effects have been reported by various authors (Ribiere et al, 1992;Wieland and Lauterburg 1995;Mansouri et al, 1999). Above 80 g ethanol per day undoubtedly alcohol has ageaccelerating and life-shortening properties (Laufs and Böhm 2003;Renaud et al, 1998).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

DCFH2 interactions with hydroxyl radicals and other oxidants – Influence of organic solvents

Brömme

Zühlke

Silber

et al. 2008

Experimental Gerontology

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ACCEPTED MANUSCRIPT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 A C C E P T E D M A N U S C R I P T

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“…Ethanol increases the generation of reactive oxygen species (ROS; Kukiełka et al, 1994). Increased ROS formation damages hepatic proteins, lipids, and DNA (Wieland and Lauterburg, 1995). Mitochondrial DNA (mtDNA) is more susceptible to oxidative damage than nuclear DNA (nDNA) due to incomplete DNA repair capacity in mitochondria and the proximity of mtDNA to the respiratory chain, a major source of ROS in the cell.…”

mentioning

confidence: 99%

Hepatic Mitochondrial DNA Depletion after an Alcohol Binge in Mice: Probable Role of Peroxynitrite and Modulation by Manganese Superoxide Dismutase

Larosche¹,

Lettéron²,

Berson³

et al. 2009

J Pharmacol Exp Ther

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Alcohol consumption increases reactive oxygen species (ROS) formation, which can damage mitochondrial DNA (mtDNA) and alter mitochondrial function. To test whether manganese superoxide dismutase (MnSOD) modulates acute alcohol-induced mitochondrial alterations, transgenic MnSOD-overexpressing (MnSOD ϩϩϩ ) mice, heterozygous knockout (MnSOD ϩ/Ϫ ) mice, and wild-type (WT) littermates were sacrificed 2 or 24 h after intragastric ethanol administration (5 g/kg). Alcohol administration further increased MnSOD activity in MnSOD ϩϩϩ mice, but further decreased it in MnSOD ϩ/Ϫ mice. In WT mice, alcohol administration transiently increased mitochondrial ROS formation, decreased mitochondrial glutathione, depleted and damaged mtDNA, and decreased complex I and V activities; alcohol durably increased inducible nitric-oxide synthase (NOS) expression, plasma nitrites/nitrates, and the nitration of tyrosine residues in complex V proteins. These effects were prevented in MnSOD ϩϩϩ mice and prolonged in MnSOD ϩ/Ϫ mice. In alcoholized WT or MnSOD ϩ/Ϫ mice, mtDNA depletion and the nitration of tyrosine residues in complex I and V proteins were prevented or attenuated by cotreatment with tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), a superoxide scavenger; N

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Oxidation of Mitochondrial Proteins and DNA Following Administration of Ethanol

Cited by 89 publications

References 0 publications

Increased Mitochondrial DNA Content in Saliva Associated with Head and Neck Cancer

Increased Mitochondrial DNA Content in Saliva Associated with Head and Neck Cancer

DCFH2 interactions with hydroxyl radicals and other oxidants – Influence of organic solvents

Hepatic Mitochondrial DNA Depletion after an Alcohol Binge in Mice: Probable Role of Peroxynitrite and Modulation by Manganese Superoxide Dismutase

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