Oxidative Enzymes

Frič, F.

doi:10.1007/978-3-642-66279-9_22

Cited by 26 publications

(13 citation statements)

References 84 publications

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“…Although the pI values of these endosperm isozymes are similar to P8.5, their Mr values are slightly higher. In addition, mRNA coding for BP 1 (4,28). The purification to homogeneity of P8.5 and the production of an anti-P8.5 antiserum represent a first step toward investigating the role of this peroxidase in host-pathogen interactions.…”

Section: Discussionmentioning

confidence: 99%

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Purification of an Infection-Related, Extracellular Peroxidase from Barley

Kerby¹,

Somerville²

1992

Plant Physiol.

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Increases in two extracellular peroxidases were observed following inoculation of barley (Hordeum vulgare L.) with the powdery mildew pathogen (Erysiphe graminis DC.: Fr. f. sp. hordei Em. Marchal). The more prominent isozyme, P8.5, was purified from intercellular wash fluids by acetone precipitation, ion-exchange chromatography, isoelectric focusing, and gel filtration. Purified P8.5 is a heme-containing, glycoprotein with a M, of 35,000. It has eight cysteine residues. A highly specific, high-titer antiserum to deglycosylated P8.5 was produced.f. sp. hordei, the causal agent of powdery mildew of barley, results in a 16-fold increase in extracellular peroxidase activity within 3 d of inoculation. The increased activity is a consequence of an increase in two isozymes with pI values of 8.5 and 5.2, which we have named P8.5 and P5.2, respectively (11).Our long-term goal is to test the hypothesis that the pI 8.5 and pl 5.2 peroxidase isozymes are involved in the deposition of lignin-like compounds in papillae and in cross-linking phenolic or phenylpropanoid compounds into preexisting cell-wall polymers. As a step toward this goal, we report the purification of P8.5.Peroxidase isozymes, which are induced following inoculation with pathogens, are thought to be involved in the deposition of lignin-like compounds at sites of attempted penetration and wounding (6). The lignin-like deposits are

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Section: Discussionmentioning

confidence: 99%

“…It has also been suggested that peroxidases may cross-link phenolic compounds into plant cell-wall polymers and into callose-containing papillae at infection sites (5). Others have proposed that toxic radicals capable of inhibiting pathogen growth are produced extracellularly via peroxidase action (4).…”

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confidence: 99%

Purification of an Infection-Related, Extracellular Peroxidase from Barley

Kerby¹,

Somerville²

1992

Plant Physiol.

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“…(Kosuge, 1969;Farkas and Kiraly, 1962;Mayer and Hare!, 1979;Mayer, 1987). Many studies have correlated increased catecholase activity with a plant's resistance to a phytopathogen as compared to susceptible varieties (Mukherjee and Ghosh, 1975;Matta and Gentile, 1970;Maxemiuc-Naccache and Dietrich, 1985;Fric, 1976;Arora and Bajaj, 1985;Trajkovski, 1976). However, other studies of plant pathogen systems have not shown such a correlation (Brueske and Dropkin, 1973;Conti et al, 1982) or reported a higher catecholase activity in susceptible plants (Pollock and Drysdale, 1976).…”

Section: Role In Photosynthesismentioning

confidence: 99%

Diphenol Oxidases, Enzyme-catalysed Browning and Plant Disease Resistance

Walker

Ferrar

1998

Biotechnology and Genetic Engineering Reviews

159

120

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“…They did not correspond with any enzymes secreted by SAA itself, and must be assumed to be a response by the plant. The incompatible host interactions of plants are known to result in enhanced concentrations of peroxidase (Fric, 1980); also, additional isozymes of peroxidase may occur in virus infected plant tissues and are associated with the development of local lesions and general chlorosis (Bates & Chant, 1970).…”

Section: Translocation Of Salivary Secretions In the Plantmentioning

confidence: 99%

Mobility of salivary components as a possible reason for differences in the responses of alfalfa to the spotted alfalfa aphid and pea aphid

Madhusudhan

Miles

1998

Entomologia Exp Applicata

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The spotted alfalfa aphid (SAA), Therioaphis trifolii maculata (Buckton), causes a characteristic veinal chlorosis and necrosis in the growing tips of susceptible cultivars of alfalfa. The pea aphid, Acyrthosiphon pisum (Harris), causes general degenerative changes in alfalfa but no specific, local symptoms. Biochemical and electrophoretic analyses detected similar enzymes in the ejected saliva of either species: pectin methylesterase, endopolygalacturonase and at least three isozymes of a copper dependent oxido‐reductase that showed both catechol oxidase and peroxidase activity. Pectinase and catechol oxidase activities per unit of soluble protein were much greater in the saliva of pea aphid compared with that of SAA. The isozymes of the oxidase from SAA were roughly half the molecular weights of the corresponding isozymes from pea aphid, however, and radiotracer studies showed that soluble secretions injected into alfalfa by SAA travelled to growing tips considerably faster than the secretions of pea aphid. It is suggested that differences in the lesions caused by these aphids may be due to reaction kinetics rather than specific salivary toxins; that the rate of arrival of salivary components, possibly the oxidases, at phloem unloading sites may determine whether the plant's local defensive system is able to repress the immediate challenge or undergoes a run‐away reaction leading to necrosis.

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Oxidative Enzymes

Cited by 26 publications

References 84 publications

Purification of an Infection-Related, Extracellular Peroxidase from Barley

Purification of an Infection-Related, Extracellular Peroxidase from Barley

Diphenol Oxidases, Enzyme-catalysed Browning and Plant Disease Resistance

Mobility of salivary components as a possible reason for differences in the responses of alfalfa to the spotted alfalfa aphid and pea aphid

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