Oxidative Phosphorylation in Bacteria

Gel’man, Nina S.; Lukoyanova, Marina; Ostrovskii, D. N.

doi:10.1007/978-1-4899-5526-5_5

Cited by 8 publications

(3 citation statements)

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“…More and more researches indicated host’ nutrition source not only comes from food intake from outside environment, but commensal microbes also provide mirco-nutrients like folate through metabolism of undigested food residue. Glycolysis/gluconeogenesis and oxidative phosphorylation provide the needed energy for microbe to complete normal growth and routine activities [46, 47]. Unlike MSB+ and Ref, nitrogen metabolism, citrate cycle, and ABC transporters were main pathways abundant in CSB+ and Maize, indicating the metabolic direction of microbial community differs upon the source of intake.…”

Section: Discussionmentioning

confidence: 99%

Millet-based supplement restored microbiota diversity of acute undernourished pigs

Yang

Ren

et al. 2019

Preprint

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The strong connection between undernutrition and gut microbiota (GM) has enabled microbiota-targeting to become an evolving strategy, which witnessed urgent need for fortified formula of supplementary food in undernutrition therapy. Using undernourished pigs as models, we investigated how corn- and millet-based nutritional supplement acted differently in modulating microbiota. Undernourished pigs at age of 9 weeks were fed with pure maize diet (Maize), corn-based (CSB+) and millet-based (MSB+) supplementary food for 3 weeks. Compared with Maize group, both CSB+ and MSB+ improved serum total protein and globulin level, but no physiological improvement was observed by short-term food intervention. MSB+ shown superior influence on GM immaturity which were more normally-grown like at both structural and functional level. Higher level of Bacteriodetes, Firmicutes and lower level of Preteobacteria were detected in MSB+ fed piglets in contrast with CSB+. Lachnospira.spp was significantly raised after nutritional intervention, indicating high correlation with the undernutrition-associated phenotype. Thus, especially from the GM aspect, millet could be one promising source to help undernourished children reconstruct balanced microbiota in short therapeutic term.

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Section: Discussionmentioning

confidence: 99%

Millet-based supplement restored microbiota diversity of acute undernourished pigs

Yang

Ren

et al. 2019

Preprint

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“…The GM of MSB+ and Ref pigs showed distinct genetic enrichment in purine metabolism, glycolysis/gluconeogenesis, oxidative phosphorylation and folate biosynthesis. The improved microbial diversity shall take account for the enriched central energy metabolism pathways e.g., purine metabolism, glycolysis/gluconeogenesis and oxidative phosphorylation, suggesting a more active and cooperative bacterial food web [ 45 , 46 ]. Meanwhile, the food intake allows commensal microbes to complete necessary metabolism and biosynthesis, which nurtures the host.…”

Section: Discussionmentioning

confidence: 99%

Millet-based supplement restored gut microbial diversity of acute malnourished pigs

Hui

Leng

et al. 2021

PLoS ONE

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The tight association between malnutrition and gut microbiota (GM) dysbiosis enables microbiota-targeting intervention to be a promising strategy. Thus, we used a malnourished pig model to investigate the host response and GM alterations under different diet supplementation strategies. Pigs at age of 4 weeks were fed with pure maize diet to induce malnutrition symptoms, and followed by continuous feeding with maize (Maize, n = 8) or re-feeding using either corn-soy-blend (CSB+, n = 10) or millet-soy-blend based (MSB+, n = 10) supplementary food for 3 weeks. Meanwhile, 8 pigs were fed on a standard formulated ration as control (Ref). The effect of nutritional supplementation was assessed by the growth status, blood chemistry, gastrointestinal pathology, mucosal microbiota composition and colon production of short-chain fatty acids. Compared with purely maize-fed pigs, both CSB+ and MSB+ elevated the concentrations of total protein and globulin in blood. These pigs still showed most malnutrition symptoms after the food intervention period. MSB+ had superior influence on the GM development, exhibiting better performance in both structural and functional aspects. MSB+ pigs were colonized by less Proteobacteria but more Bacteroidetes, Firmicutes and Lachnospira spp. Pearson’s correlation analysis indicated a strong correlation between the abundance of mucosal e.g., Faecalibacterium and Lachnospira spp. and body weight, crown-rump length and total serum protein. In conclusion, the malnutrition symptoms were accompanied by an aberrant GM, and millet-based nutritional supplementation showed promising potentials to restore the reduced GM diversity implicated in pig malnutrition.

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“…It must also be noted that oxidative phosphorylation (OXPHOS) is responsible for producing energy rich ATP for the proliferation of any cellular system including microorganisms. Generally, in the case of eukaryotic cells, OXPHOS will occur in mitochondria [30]. However, in the case of bacteria, the enzyme involved in electron transport and OXPHOS reside on the bacterial inner membrane, cytoplasmic membrane [31,32].…”

Section: Optical Reduction and Oxidation Ratiomentioning

confidence: 99%

Characterization and classification of pathogenic bacteria using native fluorescence and spectral deconvolution

Sundaramoorthy,

Bharanidharan,

Prakasarao

et al. 2024

Journal of Biophotonics

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Identification and classification of pathogenic bacterial strains is of current interest for the early treatment of diseases. In this work, protein fluorescence from eight different pathogenic bacterial strains were characterized using steady state and time resolved fluorescence spectroscopy. The spectral deconvolution method was also employed to decompose the emission contribution from different intrinsic fluorophores and extracted various key parameters, such as intensity, emission maxima, emission line width of the fluorophores, and optical redox ratio. The change in average lifetime values across different bacterial strains exhibits good statistical significance (p ≤ 0.01). The variations in the photophysical characteristics of bacterial strains are due to the different conformational states of the proteins. The stepwise multiple linear discriminate analysis of fluorescence emission spectra at 280 nm excitation across eight different bacterial strains classifies the original groups and cross validated group with 100% and 99.5% accuracy, respectively.

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Oxidative Phosphorylation in Bacteria

Cited by 8 publications

References 0 publications

Millet-based supplement restored microbiota diversity of acute undernourished pigs

Millet-based supplement restored microbiota diversity of acute undernourished pigs

Millet-based supplement restored gut microbial diversity of acute malnourished pigs

Characterization and classification of pathogenic bacteria using native fluorescence and spectral deconvolution

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