Oxidative Phosphorylation System in Gastric Carcinomas and Gastritis

Feichtinger, René G.; Neureiter, Daniel; Skaria, Tom; Weßler, Silja; Cover, Timothy L.; Mayr, Johannes A.; Zimmermann, Franz; Posselt, Gernot; Sperl, Wolfgang; Kofler, Barbara

doi:10.1155/2017/1320241

Cited by 26 publications

(24 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the expression imbalance in certain gene pairs might play a more important role than individual differentially expressed genes. In GSEA, we found that “oxidative and phosphorylation” was significantly enriched in the low‐risk group, which was consistent with the recent study .…”

Section: Discussionsupporting

confidence: 92%

Identification of a novel gene pairs signature in the prognosis of gastric cancer

Peng

Zhou

et al. 2017

Cancer Medicine

View full text Add to dashboard Cite

Current prognostic signatures need to be improved in identifying high‐risk patients of gastric cancer (GC). Thus, we aimed to develop a reliable prognostic signature that could assess the prognosis risk in GC patients. Two microarray datasets of GSE662254 (n = 300, training set) and GSE15459 (n = 192, test set) were included into analysis. Prognostic genes were screened to construct prognosis‐related gene pairs (PRGPs). Then, a penalized Cox proportional hazards regression model identified seven PRGPs, which constructed a prognostic signature and divided patients into high‐ and low‐risk groups according to the signature score. High‐risk patients showed a poorer prognosis than low‐risk patients in both the training set (hazard ratios [HR]: 6.086, 95% confidence interval [CI]: 4.341–8.533) and test set (1.773 [1.107–2.840]). The PRGPs signature also achieved a higher predictive accuracy (concordance index [C‐index]: 0.872, 95% CI: 0.846–0.897) than two existing molecular signatures (0.706 [0.667–0.744] for a 11‐gene signature and 0.684 [0.642–0.726] for a 24‐lncRNA signature) and TNM stage (0.764 [0.715–0.814]). In conclusion, our study identified a novel gene pairs signature in the prognosis of GC.

show abstract

Section: Discussionsupporting

confidence: 92%

Identification of a novel gene pairs signature in the prognosis of gastric cancer

Peng

Zhou

et al. 2017

Cancer Medicine

View full text Add to dashboard Cite

show abstract

“…The most frequent alterations of the OXPHOS system in our cohort were the loss of complex I and complex V. The absence of complex I protein and potentially pathogenic mutations of mitochondrial complex I genes have been consistently reported for numerous tumor entities [ 1 , 4 – 6 , 10 , 12 , 13 , 28 , 33 ]. A very recent study sequenced mtDNA in 384 prostate carcinoma patients and identified 129 nonsynonymous mitochondrial single-nucleotide variants in protein-coding regions, including six premature stop codons and two mutated stop codons [ 25 ].…”

Section: Discussionmentioning

confidence: 59%

Reduced Levels of ATP Synthase Subunit ATP5F1A Correlate with Earlier‐Onset Prostate Cancer

Feichtinger

Schäfer

Seifarth

et al. 2018

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Switching of cellular energy production from oxidative phosphorylation (OXPHOS) to aerobic glycolysis occurs in many types of tumors. However, the significance of energy metabolism for the development of prostate carcinoma is poorly understood. We investigated the expression of OXPHOS complexes in 94 human prostate carcinomas and paired benign tissue using immunohistochemistry. Overall mitochondrial mass was upregulated in carcinomas compared to benign prostate tissue in all Gleason grades. A significant direct correlation between the expression of OXPHOS complexes I, II, and V and the Gleason score was observed. However, 17% of prostate carcinomas and 18% of benign prostate tissues showed isolated or combined deficiency of OXPHOS complexes (one deficiency in 12% of the tumors, combined deficiencies in 5%). Complex I was absent in 9% of the samples, with only parts of the tumor affected. ATP5F1A, a complex V protein, was the most frequently affected subunit, in 10% of tumors and 11% of benign prostate tissues (but not both tissues in any single patient). A possible role of complex V in prostate cancer development is suggested by the significant positive correlation of ATP5F1A levels with earlier-onset prostate cancer (age at diagnosis and at prostatectomy) and free PSA percentage. The relatively high percentage (17%) of prostate carcinomas with regional foci of partial OXPHOS complex deficiencies could have important therapeutic implications.

show abstract

“…For antigen retrieval, the sections were immersed for 45 min in 1 mM EDTA, 0.05% Tween‐20, pH 8, at 95°C. Tissue sections were incubated for 60 min with the above‐mentioned primary antibodies (Feichtinger et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…After separation, proteins were blotted on a nitrocellulose membrane (Feichtinger et al, 2014). The Western blot was incubated with antibodies in the following order: polyclonal rabbit anti-WARS2 for 60 min with the above-mentioned primary antibodies (Feichtinger et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Biallelic variants inWARS2encoding mitochondrial tryptophanyl-tRNA synthase in six individuals with mitochondrial encephalopathy

et al. 2017

Self Cite

View full text Add to dashboard Cite

Mitochondrial protein synthesis involves an intricate interplay between mitochondrial DNA encoded RNAs and nuclear DNA encoded proteins, such as ribosomal proteins and aminoacyl-tRNA synthases. Eukaryotic cells contain 17 mitochondria-specific aminoacyl-tRNA synthases. WARS2 encodes mitochondrial tryptophanyl-tRNA synthase (mtTrpRS), a homodimeric class Ic enzyme (mitochondrial tryptophan-tRNA ligase; EC 6.1.1.2). Here, we report six individuals from five families presenting with either severe neonatal onset lactic acidosis, encephalomyopathy and early death or a later onset, more attenuated course of disease with predominating intellectual disability. Respiratory chain enzymes were usually normal in muscle and fibroblasts, while a severe combined respiratory chain deficiency was found in the liver of a severely affected individual. Exome sequencing revealed rare biallelic variants in WARS2 in all affected individuals. An increase of uncharged mitochondrial tRNA and a decrease of mtTrpRS protein content were found in fibroblasts of affected individuals. We hereby define the clinical, neuroradiological, and metabolic phenotype of WARS2 defects. This confidently implicates that mutations in WARS2 cause mitochondrial disease with a broad spectrum of clinical presentation.

show abstract

Oxidative Phosphorylation System in Gastric Carcinomas and Gastritis

Cited by 26 publications

References 71 publications

Identification of a novel gene pairs signature in the prognosis of gastric cancer

Identification of a novel gene pairs signature in the prognosis of gastric cancer

Reduced Levels of ATP Synthase Subunit ATP5F1A Correlate with Earlier‐Onset Prostate Cancer

Biallelic variants inWARS2encoding mitochondrial tryptophanyl-tRNA synthase in six individuals with mitochondrial encephalopathy

Contact Info

Product

Resources

About