Oxidative Stress and Antioxidant Status in Patients with Erectile Dysfunction

Barassi, Alessandra; Colpi, Giovanni M.; Piediferro, G.; Dogliotti, Giada; d’Eril, G.V. Melzi; Corsi, Massimiliano M.

doi:10.1111/j.1743-6109.2009.01279.x

Cited by 35 publications

(28 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First of all, both the evaluation of plasma hydroperoxides and Lag-time (Figure 1) confirm previous observations showing that the condition of oxidative stress in ED patients [26] overlaps the well-known OS status in patients with other pathologies [40]. Regarding peroxidation parameters, an intriguing point is the observation that in ED subjects RBC membrane fluidity shows no significant differences between patients and controls (Figure 2).…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Levels of Human Erythrocyte Membrane-Bound and Cytosolic Glycohydrolases Are Associated with Oxidative Stress in Erectile Dysfunction Patients

et al. 2014

View full text Add to dashboard Cite

Oxidative stress (OS) and production of NO, by endothelium nitric oxide synthetase (eNOS), are involved in the pathophysiology of erectile dysfunction (ED). Moreover, OS induces modifications of the physicochemical properties of erythrocyte (RBC) plasma membranes and of the enzyme content of the same membranes. Due to their role in signalling early membrane alterations in OS-related pathologies, several plasma membrane and cytosolic glycohydrolases of human RBC have been proposed as new markers of cellular OS. In RBC, NOS can be activated and deactivated by phosphorylation/glycosylation. In this regulatory mechanism O-β-N-AcetylGlucosaminidase is a key enzyme. Cellular levels of O-GlcNAcylated proteins are related to OS; consequently dysfunctional eNOS O-GlcNAcylation seems to have a crucial role in ED. To elucidate the possible association between RBC glycohydrolases and OS, plasma hydroperoxides and antioxidant total defenses (Lag-time), cytosolic O-β-N-AcetylGlucosaminidase, cytosolic and membrane Hexosaminidase, membrane β-D-Glucuronidase, and α-D-Glucosidase have been studied in 39 ED patients and 30 controls. In ED subjects hydroperoxides and plasma membrane glycohydrolases activities are significantly increased whereas Lag-time values and cytosolic glycohydrolases activities are significantly decreased. These data confirm the strong OS status in ED patients, the role of the studied glycohydrolases as early OS biomarker and suggest their possible use as specific marker of ED patients, particularly in those undergoing nutritional/pharmacological antioxidant therapy.

show abstract

Section: Discussionsupporting

confidence: 84%

“…No ethics approval was required because no additional blood was needed for this study and this was explained thoroughly to all patients. This procedure has been accepted by several journals [26]. …”

Section: Methodsmentioning

confidence: 99%

Levels of Human Erythrocyte Membrane-Bound and Cytosolic Glycohydrolases Are Associated with Oxidative Stress in Erectile Dysfunction Patients

et al. 2014

View full text Add to dashboard Cite

show abstract

“…One cause could be a reduced activity of DDHA, highly expressed in the liver (Nijveldt et al ., 2003), whose activity may be inhibited by a large series of traditional (hypercholesterolaemia, diabetes, smoking), non‐traditional (hyperhomocysteinaemia, inflammation, oestrogen deficiency, insulin resistence, hypothyroidism) risk factors and finally by oxidative stress. A fairly recent study showed that plasma reactive oxygen metabolite (ROS) concentrations were higher and plasma total antioxidant status (TAS) was lower in ED AR patients in comparison with ED NON‐AR patients and in controls (Barassi et al ., 2009). Furthermore, through uncoupling of NOS, increased production of superoxide could further impair DDAH, leading to a perpetuating increase in ADMA concentration (Sydow et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…2005), and the peak systolic velocity (PSV) and end‐diastolic velocity (EDV) were recorded at 5, 10, 15, 20 and 25 min after the injection in the proximal portion of the penis. Patients were classified as ‘non‐arteriogenic’ when their PSV was ≥35 cm/sec, or ≤35 cm/sec, but >25 cm/sec with concomitant EDV ≤ 0 cm/sec, and ‘arteriogenic’ when their PSV was ≤20 cm/sec (Barassi A et al ., 2009; Barassi et al ., 2010). The erection quality was estimated 20 min after each injection.…”

Section: Methodsmentioning

confidence: 99%

Asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and L‐arginine in patients with arteriogenic and non‐arteriogenic erectile dysfunction

et al. 2012

View full text Add to dashboard Cite

Summary The plasma concentration of asymmetrical dimethylarginine (ADMA), an inhibitor of nitric oxide synthase, has been linked to endothelial dysfunction. We investigated the relation between ADMA, symmetric dimethylarginine (SDMA) and L‐arginine concentrations and erectile dysfunction. We compared plasma levels of ADMA, SDMA and L‐arginine in 61 men in good health with erectile dysfunction of arteriogenic and non‐arteriogenic origin. Diagnosis of erectile dysfunction was based on the International Index of Erectile Function Score and its aetiology was classified with penile echo‐colour‐Doppler in basal condition and after intracavernous injection of prostaglandin E1. The ADMA and SDMA concentrations were significantly higher in men with arteriogenic erectile dysfunction compared with those with erectile dysfunction of non‐arteriogenic origin (p < 0.05) and the concentrations in both subgroups were significantly higher than in controls (p < 0.001). There was a negative correlation between ADMA and International Index of Erectile Function Score only in arteriogenic erectile dysfunction subgroup. L‐arginine did not differ significantly neither between the two erectile dysfunction subgroups (p > 0.05) nor between each of the two erectile dysfunction subgroups and controls (p > 0.05). The L‐arginine/ADMA and the L‐arginine/SDMA ratios in arteriogenic erectile dysfunction subgroups were significantly lower than both in controls (p < 0.05) and in non‐arteriogenic erectile dysfunction patients (p < 0.05); the two ratios in non‐arteriogenic erectile dysfunction patients did not differ from those in the controls (p > 0.05). We conclude that ADMA and SDMA concentrations are significantly higher and L‐arginine/ADMA ratio lower in patients who have arteriogenic erectile dysfunction compared with both patients with non‐arteriogenic erectile dysfunction and controls. The negative correlation between ADMA and severity of erectile dysfunction is present only in patients with arteriogenic erectile dysfunction. This study supports the importance to always distinguish arteriogenic from non‐arteriogenic erectile dysfunction patients to study the complicate erectogenic mechanisms that lead to erectile dysfunction and also to provide potential therapeutic agents for patients with arteriogenic erectile dysfunction.

show abstract

“…29,30 Increased production of reactive oxygen species (such as superoxide and peroxynitrite) in subjects with cardiovascular risk factors decrease bioavailability of NO and may lead to ED. 31 The study by Barassi et al 32 found that while the concentration of plasma reactive oxygen metabolite was higher in arteriogenic ED compared with non-arteriogenic ED, plasma total antioxidant level was lower in arteriogenic ED. In the study by Sezen et al, 33 serum total oxidant level and oxidative stress index were increased, and total antioxidant level was lower in the CAE group compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the relationship between coronary artery ectasia and erectile function score

et al. 2011

View full text Add to dashboard Cite

The relation between coronary artery ectasia (CAE) and erectile dysfunction (ED) has not been studied so far. Hence, we decided to investigate the erectile function score in patients with CAE. We investigated the international index of erectile function (IIEF)-5 score in 34 men with CAE, 38 men with coronary artery disease (CAD), and 30 male controls with normal coronary arteries whose mean ages were 53.2 ± 5.6, 51.4 ± 7.8, and 49.6 ± 8.6 years, respectively. Erectile function was evaluated by the five-item version of the IIEF-5. Each question is scored from 0 to 5. CAE was defined as being without any stenotic lesions with a visual assessment of the coronary arteries showing a luminal dilatation 1.5-fold or more of the adjacent normal coronary segments. IIEF-5 scores in CAE group were found statistically significantly lower than the control group (Po0.001). There were no statistically significant differences in IIEF-5 scores between CAE and CAD groups (P ¼ 0.13). We have shown for the first time that patients with CAE have lower IIEF-5 scores compared with controls with normal coronary angiograms. Many studies reported that endothelial dysfunction in patients with CAE was more dominant than those with CAD. This study suggests that ED and CAE may be different manifestations of a common underlying vascular pathology and vasculogenic ED is frequently seen in CAE at least as much as in CAD.

show abstract

Oxidative Stress and Antioxidant Status in Patients with Erectile Dysfunction

Cited by 35 publications

References 23 publications

Levels of Human Erythrocyte Membrane-Bound and Cytosolic Glycohydrolases Are Associated with Oxidative Stress in Erectile Dysfunction Patients

Levels of Human Erythrocyte Membrane-Bound and Cytosolic Glycohydrolases Are Associated with Oxidative Stress in Erectile Dysfunction Patients

Asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and L‐arginine in patients with arteriogenic and non‐arteriogenic erectile dysfunction

Assessment of the relationship between coronary artery ectasia and erectile function score

Contact Info

Product

Resources

About