Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells

Liu, Shichang; Xu, Lei; Zhang, Tao; Ren, Guogang; Yang, Zhuo

doi:10.1016/j.tox.2009.11.012

Cited by 200 publications

(139 citation statements)

References 33 publications

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“…Our results were validated by directly counting the number of surviving cells using the trypan blue staining assay because the MTT assay is prone to artifacts under certain experimental conditions (L'Azou et al, 2008). The cell viability in cell culture medium containing non-coated TiO 2 was reduced to almost 50% of control values at the highest concentration examined (100 mg/L), which was in agreement with earlier studies of P25-TiO 2 (Liu et al, 2010;Park et al, 2008). The cell viability in the coated nano-TiO 2 (Vive Nano Titania) treatment group was unchanged at 10 mg/L, with a small effect at 100 mg/L; even higher concentrations up to 400 mg/L did not reduce the percentage of viable cells less than 80%…”

Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 89%

“…NPs (Aruoja et al, 2009;Karlsson et al, 2008;Xu et al, 2009), or one nano-sized particle either alone or versus larger sized counterparts (Liu et al, 2010;Park et al, 2008;Sohaebuddin et al, 2010;Wang et al, 2007). In the present study, we focused on the effects of one type of NP, nano-TiO 2 , with respect to its different physico-chemical properties, including particle surface modification.…”

Section: Most Of the Toxicological Results On Nps Have Been Generatedmentioning

confidence: 99%

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In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

124

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://dx.doi.org/10. 1016/j.tiv.2012.12.018 Toxicology in Vitro, 27, 2, pp. 864-873, 2012-12-28 In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells Hamzeh, Mahsa; Sunahara, Geoffrey I. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO 2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO 2 samples decrease cell viability in a mass-based concentration-and size-dependent manner, the polyacrylate-coated nano-TiO 2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO 2 model. Given the increasing production of TiO 2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.

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Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 89%

Section: Most Of the Toxicological Results On Nps Have Been Generatedmentioning

confidence: 99%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

124

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“…30,31 We therefore subsequently examined changes in intracellular reactive oxygen species levels in Chang cells treated with TiO 2 P25-70 . As shown in Figure 6A Figure 6D), mitochondrial membrane potential loss ( Figure 6E), and apoptosis-inducing factor and cytochrome c release ( Figure 6E).…”

Section: P25-70mentioning

confidence: 99%

Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation

Yoo¹,

Yoon²,

Kwon³

et al. 2012

IJN

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Background: Titanium dioxide (TiO 2 ) has been widely used in many areas, including biomedicine, cosmetics, and environmental engineering. Recently, it has become evident that some TiO 2 particles have a considerable cytotoxic effect in normal human cells. However, the molecular basis for the cytotoxicity of TiO 2 has yet to be defined. Methods and results:In this study, we demonstrated that combined treatment with TiO 2 nanoparticles sized less than 100 nm and ultraviolet A irradiation induces apoptotic cell death through reactive oxygen species-dependent upregulation of Fas and conformational activation of Bax in normal human cells. Treatment with P25 TiO 2 nanoparticles with a hydrodynamic size distribution centered around 70 nm ) together with ultraviolet A irradiation-induced caspase-dependent apoptotic cell death, accompanied by transcriptional upregulation of the death receptor, Fas, and conformational activation of Bax. In line with these results, knockdown of either Fas or Bax with specific siRNA significantly inhibited TiO 2 -induced apoptotic cell death. Moreover, inhibition of reactive oxygen species with an antioxidant, N-acetyl-L-cysteine, clearly suppressed upregulation of Fas, conformational activation of Bax, and subsequent apoptotic cell death in response to combination treatment using TiO 2 P25-70 and ultraviolet A irradiation. Conclusion:These results indicate that sub-100 nm sized TiO 2 treatment under ultraviolet A irradiation induces apoptotic cell death through reactive oxygen species-mediated upregulation of the death receptor, Fas, and activation of the preapoptotic protein, Bax. Elucidating the molecular mechanisms by which nanosized particles induce activation of cell death signaling pathways would be critical for the development of prevention strategies to minimize the cytotoxicity of nanomaterials.

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“…Submit your Manuscript | www.austinpublishinggroup.com energy metabolic pathway in aerobic condition ( Figure 6) [31]. The abnormality of citric and lactic acids may be due to induced oxidative stress in the mice due to TiO 2 NPs, which indirectly causes metabolic perturbations in energy metabolism through glycolysis process.…”

Section: Austin Publishing Groupmentioning

confidence: 99%

Evaluating Metabolic Responses in Mice to Nanosized Titanium Dioxide Particles Using Gas Chromatography- Mass Spectrometry Based Metabolomics

Mudiam¹

2017

Austin J Anal Pharm Chem

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Titanium dioxide nanoparticle (TiO 2 NP) is one of the most commonly used engineered nanoparticles. It has attracted lot of interest to analytical toxicologists in recent past due to its toxicity on human health and environment. The present study was aimed to explore the GC-MS based metabolomics as a tool to investigate the toxicity of TiO 2 NP in comparison to TiO 2 BP with doses of 300, 600, 1200 mg/kg respectively in both gender(s) of Swiss Albino mice for 7 and 14 days. Serum biochemistry and histopathology parameters were performed. Chemometric analysis by supervised PLS-DA was performed to identify the discrimination/classification between exposed and non-exposed samples due to metabolic perturbations. The morphological, biochemical, haematological and metabolomic analysis revealed that, TiO 2 NP has induced toxicological effects to both female and male mice. The results showed that metabolomics along with biochemical analysis can be employed as a comprehensive tool to identify the toxicity of NPs in the model organisms at molecular level.

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Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells

Cited by 200 publications

References 33 publications

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation

Evaluating Metabolic Responses in Mice to Nanosized Titanium Dioxide Particles Using Gas Chromatography- Mass Spectrometry Based Metabolomics

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