Oxidative Stress and Cell Signalling

Poli, Giuseppe; Leonarduzzi, Gabriella; Biasi, Fiorella; Chiarpotto, Elena

doi:10.2174/0929867043365323

Cited by 639 publications

(418 citation statements)

References 347 publications

(418 reference statements)

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“…On the other hand, it has been shown that ROS play important roles in somatic cell functions such as cell proliferation and differentiation [21,45]. Consistent with the previous finding, our results showed that increase in concentration of ALA lead to decrease ROS concentration which in turn results in decrease follicles size, survival rate, and developmental competence.…”

Section: Discussionsupporting

confidence: 92%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

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Section: Discussionsupporting

confidence: 92%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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“…In low intracellular concentrations, ROS act as secondary messengers in signal transduction pathways regulating cell growth, while high ROS concentrations induce cellular senescence and apoptosis by oxidising nucleic acids, proteins, and lipids (Poli et al 2004). In normal conditions, a dynamic balance between ROS generation and scavenging exists, resulting in a relatively low level of ROS maintained within cells.…”

Section: Introductionmentioning

confidence: 99%

A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

Zhang

Yan

et al. 2013

Cell Stress and Chaperones

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Oxidative stress may be the most significant threat to the survival of living organisms. Glutathione S-transferases (GSTs) serve as the primary defences against xenobiotic and peroxidative-induced oxidative damage. In contrast to other well-defined GST classes, the Omega-class members are poorly understood, particularly in insects. Here, we isolated and characterised the GSTO2 gene from Apis cerana cerana (AccGSTO2). The predicted transcription factor binding sites in the AccGSTO2 promoter suggested possible functions in early development and antioxidant defence. Real-time quantitative PCR (qPCR) and western blot analyses indicated that AccGSTO2 was highly expressed in larvae and was predominantly localised to the brain tissue in adults. Moreover, AccGSTO2 transcription was induced by various abiotic stresses. The purified recombinant AccGSTO2 exhibited glutathione-dependent dehydroascorbate reductase and peroxidase activities. Furthermore, it could prevent DNA damage. In addition, Escherichia coli overexpressing AccGSTO2 displayed resistance to long-term oxidative stress exposure in disc diffusion assays. Taken together, these results suggest that AccGSTO2 plays a protective role in counteracting oxidative stress.

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“…With distinctive demonstration of the involvement of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in human physiology [8,10,24,[37][38][39][40][41][42][43][44][45], the concept of ''Toxic molecules'' for free radicals FR is now outdated. In fact, there is a strong doubt if there is any acute phase FR generation is involved in etiopathogenesis of human diseases; that raised oxidative stress (OS) in majority of the disease is consequence and not the cause [6-8, 12, 18, 46-48] that raised OS is not present in all the patients [3,5,14,[49][50][51] and that a specific level of OS is necessary for vital activities [43,52,53].…”

mentioning

confidence: 99%

Reconvene and Reconnect the Antioxidant Hypothesis in Human Health and Disease

Singh

Chandra

Mahdi

et al. 2010

Indian J Clin Biochem

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The antioxidants are essential molecules in human system but are not miracle molecules. They are neither performance enhancers nor can prevent or cure diseases when taken in excess. Their supplemental value is debateable. In fact, many high quality clinical trials on antioxidant supplement have shown no effect or adverse outcomes ranging from morbidity to all cause mortality. Several Chochrane Meta-analysis and Markov Model techniques, which are presently best available statistical models to derive conclusive answers for comparing large number of trials, support these claims. Nevertheless none of these statistical techniques are flawless. Hence, more efforts are needed to develop perfect statistical model to analyze the pooled data and further double blind, placebo controlled interventional clinical trials, which are gold standard, should be implicitly conducted to get explicit answers. Superoxide dismutase (SOD), glutathione peroxidase and catalase are termed as primary antioxidants as these scavenge superoxide anion and hydrogen peroxide. All these three enzymes are inducible enzymes, thereby inherently meaning that body increases or decreases their activity as per requirement. Hence there is no need to attempt to manipulate their activity nor have such efforts been clinically useful. SOD administration has been tried in some conditions especially in cancer and myocardial infarction but has largely failed, probably because SOD is a large molecule and can not cross cell membrane. The dietary antioxidants, including nutrient antioxidants are chain breaking antioxidants and in tandem with enzyme antioxidants temper the reactive oxygen species (ROS) and reactive nitrogen species (RNS) within physiological limits. Since body is able to regulate its own requirements of enzyme antioxidants, the diet must provide adequate quantity of non-enzymic antioxidants to meet the normal requirements and provide protection in exigent condition. So far, there is no evidence that human tissues ever experience the torrent of reactive species and that in chronic conditions with mildly enhanced generation of reactive species, the body can meet them squarely if antioxidants defense system in tissues is biochemically optimized. We are not yet certain about optimal levels of antioxidants in tissues. Two ways have been used to assess them: first by dietary intake and second by measuring plasma levels. Lately determination of plasma/serum level of antioxidants is considered better index for diagnostic and prognostic purposes. The recommended levels for vitamin A, E and C and beta carotene are 2.2-2.8 lmol/l; 27.5-30 lmol/l; 40-50 lmol/l and 0.4-0.5 lmol/l, respectively. The requirement and recommended blood levels of other dietary antioxidants are not established. The resolved issues are (1) essential to scavenge excess of radical species (2) participants in redox homeostasis (3) selective antioxidants activity against radical species (4) there is no universal antioxidant and 5) therapeutic value in case of deficiency. The overar...

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Oxidative Stress and Cell Signalling

Cited by 639 publications

References 347 publications

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

Reconvene and Reconnect the Antioxidant Hypothesis in Human Health and Disease

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