Oxidative Stress and Genotoxicity in Pterygium: A Systemic Investigation

Abstract

Objectives: To perform a systemic investigation on oxidative stress and DNA damage in patients with primary pterygium. Methods: This prospective cross-sectional study included 32 patients with primary pterygium (60.1±2.0 years of age) and 33 age- and sex-matched (58.8±2.2 years of age) control subjects (P>0.05). A commercial kit was used for measuring serum total oxidant status (TOS) and total antioxidant status (TAS). The comet assay was performed after lymphocyte isolation from venous blood to quantitate DNA damage. Tail length (TL), tail intensity (TI), and tail moment (TM) were used for statistical analysis as parameters of DNA damage. Results: In the pterygium group, TOS and TAS were significantly higher when compared with those of the control group (P=0.019 and P=0.005, respectively). In terms of DNA damage, patients with pterygium had higher TL, TI, and TM than in the control subjects (P<0.0001 for all). Conclusions: Although current literature focuses on local factors in pterygium pathogenesis, patients with pterygium seem to have increased systemic oxidative status (and compensatory antioxidant response) and genotoxicity, which might create a predisposition for pterygium development.

Dynamic Thiol Disulphide Homeostasis in Patients with Surfer’s Eye: A Case-Control Study

“…Among above mentioned studies, the research conducted by Kilic-Toprak et al [26] is similar to our study. However, our work is more comprehensive compared to it because thiols are the most important reductant compounds in organisms and TDH enables dynamic regulation, performs redox signaling, and sits in a key position as a spot of oxidative stress, it is a useful marker as a clinical measure of oxidative stress in blood serum than TAS and TOS.…”

“…The authors of this study argued that these parameters could also take part in the pathogenesis of ocular complications related to systemic chemotherapy such as retinal degeneration, glaucoma, cataract, blepharitis, pterygium, macular degeneration and retinitis pigmentosa. In the third study evaluating serum total oxidant status (TOS) and total antioxidant status (TAS), Kilic-Toprak et al [26] claimed that suitable environment for pterygium progress might be created by surged systemic oxidative state and offsetting antioxidant feedback.…”

Oxidative and antioxidative stress markers in keratoconus: a systematic review and meta‐analysis

“…2018), pinguecula and pterygia (Tenkate 1999; Kilic‐Toprak et al. 2019), diabetes retinopathy or age‐related macular degeneration (Tangvarasittichai & Tangvarasittichai 2019). Fortunately, the ocular surface is rich in enzymatic and nonenzymatic antioxidant molecules to scavenge, neutralize and repair the oxidative aggressions of ROS (Shoham et al.…”

Potential Protective and Therapeutic Roles of the Nrf2 Pathway in Ocular Diseases: An Update

“…In patients with pterygium, the serum total oxidant status (TOS), total antioxidant status (TAS), and nitric oxide (NO) and MDA content were significantly increased, and the antioxidant enzyme (SOD, CAT, and GPX) content was decreased [54,55], indicating an increase in nonenzyme antioxidant activity. Meanwhile, the DNA damage parameters tail length (TL), tail intensity (TI), and tail moment were significantly increased [56]. Immunohistochemical staining for 8-OHDG in the nucleus showed more extensive and deeper staining than that observed in the normal conjunctiva.…”