Oxidative stress and immunosenescence in spleen of obese mice can be reversed by 2‐hydroxyoleic acid

Gheorghe, Alina; Heredia, Fátima Pérez de; Hunsche, Caroline; Redondo, Noemí; Díaz, L. E.; Hernández, O.; Marcos, Ascensión; Fuente, Mónica De la

doi:10.1113/ep086157

Cited by 18 publications

(9 citation statements)

References 58 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice fed on an obesogenic diet show an increased XO activity in spleen together with a higher level of oxidative stress and lower level of cellular immune response as compared to the control group. These alterations support the hypothesis that obesity-induced oxidative stress is associated with early immunosenescence [74].…”

Section: Animal Studiessupporting

confidence: 84%

Pro-Aging Effects of Xanthine Oxidoreductase Products

et al. 2020

View full text Add to dashboard Cite

The senescence process is the result of a series of factors that start from the genetic constitution interacting with epigenetic modifications induced by endogenous and environmental causes and that lead to a progressive deterioration at the cellular and functional levels. One of the main causes of aging is oxidative stress deriving from the imbalance between the production of reactive oxygen (ROS) and nitrogen (RNS) species and their scavenging through antioxidants. Xanthine oxidoreductase (XOR) activities produce uric acid, as well as reactive oxygen and nitrogen species, which all may be relevant to such equilibrium. This review analyzes XOR activity through in vitro experiments, animal studies and clinical reports, which highlight the pro-aging effects of XOR products. However, XOR activity contributes to a regular level of ROS and RNS, which appears essential for the proper functioning of many physiological pathways. This discourages the use of therapies with XOR inhibitors, unless symptomatic hyperuricemia is present.

show abstract

Section: Animal Studiessupporting

confidence: 84%

Pro-Aging Effects of Xanthine Oxidoreductase Products

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In this context, the spleen is the main site for B cells to complete their maturation, 42 nevertheless obesity itself can impact the splenic leukocyte function and proliferation, mainly B and T cells. [43][44][45] Interestingly, our results demonstrate that OVA itself is not able to affect B-cell populations in the spleen; however, when associated with obesity, the allergen affected not only their frequency, but also their activation status. Hence, the obese-allergic splenic B-cell population maintains a regulatory profile after the challenges, as shown by the high frequency of PDL1 + and PD1 + cells, associated with the lower It is well established that the expression of PD1 and its ligand PDL1, on B cells, is an important mark of immune response regulation.…”

Section: Discussionmentioning

confidence: 57%

“…B‐cell activation in the lymphoid organs is a critical point in antibody production, so the evaluation of their frequency can provide a good insight of how obesity may be affecting the general allergic response. In this context, the spleen is the main site for B cells to complete their maturation, nevertheless obesity itself can impact the splenic leukocyte function and proliferation, mainly B and T cells . Interestingly, our results demonstrate that OVA itself is not able to affect B‐cell populations in the spleen; however, when associated with obesity, the allergen affected not only their frequency, but also their activation status.…”

Section: Discussionmentioning

confidence: 65%

“…The above‐mentioned results indicate that MLNs and spleen present contrasting results in obese‐allergic mice, probably due to a strong influence of obesity over the spleen, whereas the allergen preferentially affected the MLNs. Despite the lack of studies approaching the influence of obesity on B cells during the development of allergic responses, a disturbance of obese splenic B‐cell activation and antibody production has already been demonstrated in a murine influenza infection model and upon Staphylococcus aureus infection …”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Obesity affects peripheral lymphoid organs immune response in murine asthma model

et al. 2019

View full text Add to dashboard Cite

Summary Asthma and obesity present rising incidence, and their concomitance is a reason for concern, as obese individuals are usually resistant to conventional asthma treatments and have more exacerbation episodes. Obesity affects several features in the lungs during asthma onset, shifting the T helper type 2 (Th2)/eosinophilic response towards a Th17/neutrophilic profile. Moreover, those individuals can present reduced atopy and delayed cytokine production. However, the impact of obesity on follicular helper T (Tfh) cells and B cells that could potentially result in antibody production disturbances are still unclear. Therefore, we aimed to assess the peripheral response to ovalbumin (OVA) in a concomitant model of obesity and asthma. Pulmonary allergy was induced, in both lean and obese female BALB/c mice, through OVA sensitizations and challenges. Mediastinal lymph nodes (MLNs) and spleen were processed for immunophenotyping. Lung was used for standard allergy analysis. Obese‐allergic mice produced less anti‐OVA IgE and more IgG2a than lean‐allergic mice. Dendritic cells (CD11c+ MHCIIhigh) expressed less CD86 and more PDL1 in obese‐allergic mice compared with lean‐allergic mice, in the MLNs. Meanwhile, B cells (CD19+ CD40+) were more frequent and the amount of PDL1/PD1+ cells was diminished by obesity, with the opposite effects in the spleen. Tfh cells (CD3+ CD4+ CXCR5+ PD1+) expressing FoxP3 were more frequent in obese mice, associated with the predominance of Th (CD3+ CD4+) cells expressing interleukin‐4/GATA3 in the MLNs and interleukin‐17A/RORγT in the spleen. Those modifications to the main components of the germinal centers could be resulting in the increased IgG2a production, which – associated with the Th17/neutrophilic profile – contributes to asthma worsening and represents an important target for future treatment strategies.

show abstract

“…Researches showed that obesity induced oxidative stress and obesity may be a state of chronic oxidative stress, an increase in NO and MDA contents, and a significant decrease in activities of SOD, CAT, and GPx [19,20]. Gheorghe et al [21] found that the spleen redox balance in obese mice was broken by reducing GSH levels and the GSH/GSSG ratio and increasing GSSG activity in the spleen, further inducing the changes of spleen immune functions. The present study showed that activities of GSH-Px, SOD, and CAT in DIO mice were lower than those in normal mice and MDA content was higher, consistent with previous studies [22,23], suggesting that HFD altered the oxidant-antioxidant balance and increased the splenic oxidation level.…”

Section: Discussionmentioning

confidence: 99%

Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli

Fang

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

Obese mice exhibited more lymphocytes in the bronchoalveolar lavage fluid and milder lung injury after Escherichia coli (E. coli) infection. However, it remained unclear whether the spleen contributed to the effect of obese mice with infection. The study was purposed to reveal the histopathological changes of the spleen caused by oxidative stress and inflammation in diet-induced obesity (DIO) mice challenged by Escherichia coli. After infection, the spleen tissues were obtained in normal and DIO mice at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. Results revealed that DIO mice have higher contents of resistin, TNF-α, IL-6, and IL-1β in the spleen than normal mice and lower concentrations of GSH-Px, SOD, and CAT and higher MDA than normal mice. After an intranasal drip of E. coli, the activities of GSH-Px, SOD, and CAT in the DIO mice were elevated and the content of MDA declined. The activities of SOD and CAT in the normal mice declined, and the content of MDA was elevated. Moreover, the contents of TNF-α, IL-6, and IL-1β in the spleen declined in DIO mice at 24 and 72 h, although the contents of leptin, resistin, TNF-α, IL-6, and IL-1β were elevated at 12 h. The contents of resistin, TNF-α, IL-6, and IL-1β were elevated in normal mice at 12 and 24 h. Those results indicated that obesity elevated splenic oxidation and inflammatory levels, but it enhanced antioxidant capacity and reduced cytokine levels of the spleen in mice to resist splenic injury after an intranasal drip of E. coli.

show abstract

Oxidative stress and immunosenescence in spleen of obese mice can be reversed by 2‐hydroxyoleic acid

Cited by 18 publications

References 58 publications

Pro-Aging Effects of Xanthine Oxidoreductase Products

Pro-Aging Effects of Xanthine Oxidoreductase Products

Obesity affects peripheral lymphoid organs immune response in murine asthma model

Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli

Contact Info

Product

Resources

About