Oxidative stress and LINE-1 reactivation in bladder cancer are epigenetically linked through active chromatin formation

Whongsiri, Patcharawalai; Pimratana, Chaowat; Wijitsettakul, Udomsak; Sanpavat, Anapat; Jindatip, Depicha; Hoffmann, Michèle J.; Goering, Wolfgang; Schulz, Wolfgang A.; Boonla, Chanchai

doi:10.1016/j.freeradbiomed.2019.01.031

Cited by 27 publications

(15 citation statements)

References 35 publications

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“…Mitochondrial dysfunction and oxidative stress are characteristic features of a number of diseases such as some forms of ataxia, neurodegenerative diseases (Parkinson's disease in particular), various forms of cancer, and other diseases [424][425][426][427]. Recent studies have shown that abnormalities and a deficiency of both the mitochondrial chain and oxidative stress cause DNA hypomethylation and increased L1 activity [428][429][430]. The stress sensor GABB45B gene was connected to the death of dopaminergic neurons in Parkinson's disease [431].…”

Section: Factors Affecting Changes In L1 Regulation In Neuropsychiatric Diseasesmentioning

confidence: 99%

Factors Regulating the Activity of LINE1 Retrotransposons

Protasova

Andreeva

Rogaev

2021

Genes

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LINE-1 (L1) is a class of autonomous mobile genetic elements that form somatic mosaicisms in various tissues of the organism. The activity of L1 retrotransposons is strictly controlled by many factors in somatic and germ cells at all stages of ontogenesis. Alteration of L1 activity was noted in a number of diseases: in neuropsychiatric and autoimmune diseases, as well as in various forms of cancer. Altered activity of L1 retrotransposons for some pathologies is associated with epigenetic changes and defects in the genes involved in their repression. This review discusses the molecular genetic mechanisms of the retrotransposition and regulation of the activity of L1 elements. The contribution of various factors controlling the expression and distribution of L1 elements in the genome occurs at all stages of the retrotransposition. The regulation of L1 elements at the transcriptional, post-transcriptional and integration into the genome stages is described in detail. Finally, this review also focuses on the evolutionary aspects of L1 accumulation and their interplay with the host regulation system.

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Section: Factors Affecting Changes In L1 Regulation In Neuropsychiatric Diseasesmentioning

confidence: 99%

Factors Regulating the Activity of LINE1 Retrotransposons

Protasova

Andreeva

Rogaev

2021

Genes

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“…Now we demonstrate the existence of a similar paradox between ROS-modulated chromatin structure and genome stability. Previous studies already noticed that ROS treatments can result in chromatin relaxation, which facilitates the transcription of DNA repair proteins and ultimately ensures genome stability and cell survival against ROS (Wang et al 2020b;Whongsiri et al 2019). Our findings, however, have demonstrated a novel pathway by which hydrogen peroxide can interrupt the TIP60-SUV39H1 interaction, thus relaxing the heterochromatin and compromising genome instability.…”

Section: Discussionmentioning

confidence: 49%

TIP60 recruits SUV39H1 to chromatin to maintain heterochromatin genome stability and resist hydrogen peroxide-induced cytotoxicity

Bao

Tang

et al. 2020

GENOME INSTAB. DIS.

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Tat interacting protein 60 (TIP60) is a histone acetyltransferase involved in chromatin remodeling and the DNA damage response. However, the role of TIP60 in maintaining genome stability is poorly understood. Here, we show that TIP60 can directly interact with suppressor of variegation 3-9 homologue 1 (SUV39H1), a methyltransferase that is responsible for histone H3 tri-methylation on Lys9 (H3K9me3). When we knocked down TIP60 or treated cells with hydrogen peroxide, a typical reactive oxygen species (ROS) generator that induces genome instability, SUV39H1 dissociated from chromatin but its acetylation levels remained unchanged. Consequently, H3K9me3 levels in the heterochromatin decreased, leading to a significant increase in the expression of satellite 2 (Sat2) and α-satellite (α-Sat), indicators of heterochromatin relaxation. Micrococcal nuclease sensitivity and colony formation assays further demonstrated that TIP60 knockdown or hydrogen peroxide treatment resulted in a relaxing of the heterochromatin and genome instability. Exogenous TIP60 could rescue the assembly of SUV39H1 on chromatin and ensure genome stability in response to hydrogen peroxide. This study is the first to describe a role for TIP60 in maintaining heterochromatin structure and genome stability by recruiting SUV39H1 to the chromatin upon oxidative stress, presenting TIP60 as a promising target to sensitize cancer cells to ROS-promoting therapies.

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“…Protein carbonyl content was normalised by total protein concentration in the sample. Based on our previous report, variations of inter‐assay and intra‐assay (%CV) were 8.9% and 11.7% respectively (Whongsiri et al., 2019).…”

Section: Participants and Methodsmentioning

confidence: 92%

“…Detection of protein carbonyl is based on the reaction of carbonyl groups with 2,4‐dinitrophenylhydrazine (DNPH) to yield a hydrazone product. The reaction was performed according to our earlier reports (Poungpairoj et al., 2015; Whongsiri, Phoyen, & Boonla, 2018; Whongsiri et al., 2019). Spermatozoa were lysed with RIPA buffer.…”

Section: Participants and Methodsmentioning

confidence: 99%

Supplementing post‐wash asthenozoospermic human spermatozoa with coenzyme Q10 for 1 hr in vitro improves sperm motility, but not oxidative stress

et al. 2020

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We investigated the effect of supplementing post‐wash asthenozoospermic spermatozoa with coenzyme Q10 (CoQ10) in vitro, which may reduce oxidative stress and improve sperm motility. Semen samples were collected from 39 men with asthenozoospermia, and their spermatozoa were isolated by two‐layer Percoll density‐gradient centrifugation. Kinetic parameters of the isolated spermatozoa (baseline before intervention) were determined immediately by computer‐aided semen analysis. Total anti‐oxidant capacity and protein carbonyl levels, as markers of oxidative stress, were also measured in the baseline spermatozoa. The baseline spermatozoa suspension was divided equally into two portions, one for CoQ10 supplementation (50 µg/ml for 1 hr) and the other as an un‐supplemented vehicle control. The total motility of the CoQ10‐supplemented spermatozoa was significantly higher than in the control (p = .009) and progressive motility tended to be higher (p = .053). Immotile sperm concentration in the CoQ10‐supplemented spermatozoa was significantly lower than in both the baseline (p = .026) and control (p = .009). Total anti‐oxidant capacity and protein carbonyl levels between the baseline, CoQ10‐supplemented and control spermatozoa were not significantly different. Our data suggest that CoQ10 treatment reactivated sperm motility. We propose short‐term supplementation of post‐wash asthenozoospermic spermatozoa with CoQ10 before intrauterine insemination.

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Oxidative stress and LINE-1 reactivation in bladder cancer are epigenetically linked through active chromatin formation

Cited by 27 publications

References 35 publications

Factors Regulating the Activity of LINE1 Retrotransposons

Factors Regulating the Activity of LINE1 Retrotransposons

TIP60 recruits SUV39H1 to chromatin to maintain heterochromatin genome stability and resist hydrogen peroxide-induced cytotoxicity

Supplementing post‐wash asthenozoospermic human spermatozoa with coenzyme Q10 for 1 hr in vitro improves sperm motility, but not oxidative stress

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