Oxidative stress during the cryopreservation of Passiflora suberosa L. shoot tips using the V-Cryo-plate technique: determination of the critical stages of the protocol

Vianna, Marco Tadeu Gomes; Garcia, Renata; Mansur, Elisabeth; Engelmann, Florent; Pacheco, Graziela Drociunas

doi:10.1007/s11240-019-01690-8

Cited by 19 publications

(19 citation statements)

References 51 publications

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“…ROS-induced oxidative stress is a major reason of low survival in samples after cryopreservation [ 10 , 46 – 53 ]. In many species cryopreservation, H 2 O 2 is the major component of ROS leading to oxidative stress [ 8 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

et al. 2020

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Background Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field. Results This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during the dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect the ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT, and GSH than the control group EC. The EC mainly depended on the AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. The elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation. Conclusions The SWCNTs regulated oxidative stress responses of EC during the process and controlled oxidative damages by the maintenance of ROS homeostasis to achieve a high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.

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Section: Discussionmentioning

confidence: 99%

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

et al. 2020

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“…Effective antioxidant response during dehydration step improved EC survival after cryopreservation ROS-induced oxidative stress is a major reason of low survival in samples after cryopreservation [40][41][42][43][44][45][46][47][48]. In many species cryopreservation, H 2 O 2 is the major component of ROS leading to oxidative stress [8,45].…”

Section: Discussionmentioning

confidence: 99%

“…Membrane lipids are the primary target in oxidative damage [49], and MDA acting as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa [47,50], Azadirachta indica [51], Arabidopsis thaliana [8] and Agapanthus praecox [44], Hancornia speciose [52], Passi ora suberosa [48].…”

Section: Discussionmentioning

confidence: 99%

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

Ren

Deng

Chu

et al. 2020

Preprint

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Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field.Results: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration, and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT and GSH than the control group EC. EC mainly depended on AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. Elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation.Conclusions: SWCNTs regulated oxidative stress responses of EC during the process, and controlled oxidative damages by the maintenance of ROS homeostasis to achieve high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.

show abstract

“…Effective antioxidant response during the dehydration step improved EC survival after cryopreservation ROS-induced oxidative stress is a major reason of low survival in samples after cryopreservation [46][47][48][49][50][51][52][53][54]. In many species cryopreservation, H 2 O 2 is the major component of ROS leading to oxidative stress [8,51].…”

Section: Discussionmentioning

confidence: 99%

“…Membrane lipids are the primary target in oxidative damage [55], and MDA acting as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa [53,56], Azadirachtaindica [57], Arabidopsisthaliana [8], Agapanthus praecox [50], Hancornia speciose [58], and Passi ora suberosa [54]. With the addition of NRSP in the cryoprotectant, the MDA content decreased and the SOD activity increased leading to more intact membrane and better quality of boar sperm [21].…”

Section: Discussionmentioning

confidence: 99%

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

Ren

Deng

Chu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field.Results: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during the dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect the ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT, and GSH than the control group EC. The EC mainly depended on the AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. The elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation.Conclusions: The SWCNTs regulated oxidative stress responses of EC during the process and controlled oxidative damages by the maintenance of ROS homeostasis to achieve a high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.

show abstract

Oxidative stress during the cryopreservation of Passiflora suberosa L. shoot tips using the V-Cryo-plate technique: determination of the critical stages of the protocol

Cited by 19 publications

References 51 publications

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

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