Oxidative Stress in Children with Bacterial Meningitis

Srivastava, R D; Lohokare, Rajeev; Prasad, Rajniti

doi:10.1093/tropej/fmt013

Cited by 11 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous studies, we showed that increased oxidative stress is related to increased inflammatory cytokines, Helicobacter infection, and bacterial meningitis [22, 23]. In present study it is shown that SPA, TOS, and OSI were significantly increased and TAS was significantly decreased in patients with T2DM, DN, and ESRD than healthy subjects.…”

Section: Discussionsupporting

confidence: 60%

Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

Verma

Singh

et al. 2014

Biochemistry Research International

View full text Add to dashboard Cite

Association of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concerned with T2DM. 50 healthy volunteers, 50 patients with T2DM, 86 patients with DN, and 43 patients with ESRD were considered as control-1, control-2, case-1, and case-2, respectively. Blood glucose, creatinine, SPA, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured by colorimetric tests. SPA, TOS, and OSI were significantly increased in case-1 and case-2 than control-1 and control-2, while TAS was significantly decreased (P < 0.001). Blood glucose was linearly correlated to SPA, TOS, TAS, and OSI in control-2, case-1 and case-2 (P < 0.001). Serum creatinine was linearly correlated with SPA, TOS, TAS and OSI in control-2 and case-1 (P < 0.001). In case-2, serum creatinine was significantly correlated with SPA only (P < 0.001). Thus, the study concluded that SPA and oxidative stress significantly correlated with blood glucose and creatinine. SPA, TOS, TAS, and OSI can be used as biomarkers for diagnosis of kidney damage.

show abstract

Section: Discussionsupporting

confidence: 60%

Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

Verma

Singh

et al. 2014

Biochemistry Research International

View full text Add to dashboard Cite

show abstract

“…A decrease in enzymatic defense, such as SOD and CAT levels, was observed in both the PFC and hippocampus of the meningitis rats. Srivastava et al have reported clinical evidence for an increase in MDA, protein carbonyl, and nitrite levels and a decrease in enzymatic defense levels in the plasma and CSF of meningitis patients [48]. We also measured mitochondrial complex I, II, III, and IV activity in the PFC and hippocampus, and in the 24-h group, we found a decrease in complex III activity in the meningitis group compared to that in the control group (data not shown).…”

Section: Discussionmentioning

confidence: 56%

Neuroinflammation trajectories precede cognitive impairment after experimental meningitis—evidence from an in vivo PET study

Giridharan

Collodel²,

Generoso³

et al. 2020

J Neuroinflammation

View full text Add to dashboard Cite

Background: Bacterial meningitis is a devastating central nervous system (CNS) infection with acute and long-term neurological consequences, including cognitive impairment. The aim of this study was to understand the association between activated microglia-induced neuroinflammation and post-meningitis cognitive impairment. Method: Meningitis was induced in male Wistar rats by injecting Streptococcus pneumoniae into the brain through the cisterna magna, and rats were then treated with ceftriaxone. Twenty-four hours and 10 days after meningitis induction, rats were imaged with positron emission tomography (PET) using [ 11 C]PBR28, a specific translocator protein (TSPO) radiotracer, to determine in vivo microglial activation. Following imaging, the expression of TSPO, cardiolipin, and cytochrome c, inflammatory mediators, oxidative stress markers, and glial activation markers were evaluated in the prefrontal cortex and hippocampus. Ten days after meningitis induction, animals were subjected to behavioral tests, such as the open-field, step-down inhibitory avoidance, and novel object recognition tests. Results: Both 24-h (acute) and 10-day (long-term) groups of rats demonstrated increased [ 11 C]PBR28 uptake and microglial activation in the whole brain compared to levels in the control group. Although free from infection, 10day group rats exhibited increased expression levels of cytokines and markers of oxidative stress, microglial activation (IBA-1), and astrocyte activation (GFAP) similar to those seen in the 24-h group. Acute meningitis induction also elevated TSPO, cytochrome c, and caspase-3 levels with no change in caspase-9 levels. Furthermore, upregulated levels of TSPO, cytochrome c, and caspase-3 and caspase-9 were observed in the rat hippocampus 10 days after meningitis induction with a simultaneous reduction in cardiolipin levels. Animals showed a cognitive decline in all tasks compared with the control group, and this impairment may be at least partially mediated by activating a glia-mediated immune response and upregulating TSPO. Conclusions: TSPO-PET could potentially be used as an imaging biomarker for microglial activation and long-term cognitive impairment post-meningitis. Additionally, this study opens a new avenue for the potential use of TSPO ligands after infection-induced neurological sequelae.

show abstract

“…In many CNS diseases, such as traumatic brain injury (TBI), stroke, hydrocephalus, and post-hemorrhagic and post-ischemic brain damage, lipid peroxidation has been demonstrated to play a key role in brain damage [11]. Even CNS infection pathophysiology has been demonstrated to involve oxidative stress [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…In our study, we have interestingly shown that brain-specific increase in lipid peroxidationevaluated into the CSF through serial sampling-exists soon after surgical injury, since no significant MDA or ascorbate variations exist in systemic plasma samples obtained by contemporary collection despite a marked postoperative inflammatory response (witnessed by IL-6 and IL-8 plasma levels increase). The issue of brain-limited oxidative event has been debated in comparison with the available literature: In our opinion, an extensive BBB breakage is only present in severely inflamed brain, as in meningitis-meningoencephalitis patients [12,13]. Therefore, most papers dealing with MDA increase in plasma refer to diffusely involved endothelium.…”

Section: Discussionmentioning

confidence: 99%

Lipid Peroxidation and Antioxidant Consumption as Early Markers of Neurosurgery-Related Brain Injury in Children

et al. 2019

View full text Add to dashboard Cite

Background and Aims Lipid peroxidation represents a marker of secondary brain injury both in traumatic and in nontraumatic conditions-as in major neurosurgical procedures-eventually leading to brain edema amplification and further brain damage. Malondialdehyde (MDA), a lipid peroxidation marker, and ascorbate, a marker of antioxidant status, can represent early indicators of this process within the cerebrospinal fluid (CSF). We hypothesized that changes in cerebral lipid peroxidation can be measured ex vivo following neurosurgery in children. Methods Thirty-six children (M:F = 19/17, median age 32.9 months; IQR 17.6-74.6) undergoing neurosurgery for brain tumor removal were admitted to the pediatric intensive care unit (PICU) in the postoperative period with an indwelling intraventricular catheter for intracranial pressure monitoring and CSF drainage. Plasma and CSF samples were obtained for serial measurement of MDA, ascorbate, and cytokines. Results An early brain-limited increase in lipid peroxidation was measured, with a significant increase from baseline of MDA in CSF (p = 0.007) but not in plasma. In parallel, ascorbate in CSF decreased (p = 0.05). Systemic inflammatory response following brain surgery was evidenced by plasma IL-6/IL-8 increase (p 0.0022 and 0.0106, respectively). No correlation was found between oxidative response and tumor site or histology (according to World Health Organization grading). Similarly, lipid peroxidation was unrelated to the length of surgery (mean 321 ± 73 min), or intraoperative blood loss (mean 20.9 ± 16.8% of preoperative volemia, 44% given hemotransfusions). Median PICU stay was 3.5 days (IQL range 2-5.5 d.), and postoperative ventilation need was 24 h (IQL range 20-61.5 h). The elevation in postoperative MDA in CSF compared with preoperative values correlated significantly with postoperative ventilation need (P = 0.05, r2 0168), while no difference in PICU stay was recorded. Conclusions Our results indicate that lipid peroxidation increases consistently following brain surgery, and it is accompanied by a decrease in antioxidant defences; intraventricular catheterization offers a unique chance of oxidative process monitoring. Further studies are needed to evaluate whether monitoring post-neurosurgical oxidative stress in CSF is of prognostic utility.

show abstract

Oxidative Stress in Children with Bacterial Meningitis

Cited by 11 publications

References 19 publications

Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

Neuroinflammation trajectories precede cognitive impairment after experimental meningitis—evidence from an in vivo PET study

Lipid Peroxidation and Antioxidant Consumption as Early Markers of Neurosurgery-Related Brain Injury in Children

Contact Info

Product

Resources

About