Oxidative stress, inflammation, and glycometabolism disorder‐induced erythrocyte hemolysis in selenium‐deficient exudative diathesis broilers

Abstract

Selenium (Se) deficiency causes injury of diversified tissues and cells, including livers, hearts, skeletal muscles, and erythrocytes. The aim of the present study is to explore the molecular mechanism of erythrocyte hemolysis due to Se deficiency in broilers. One hundred and eighty broilers (male/female, 1 day old) were randomly divided into two groups and fed with either a normal Se content diet (C group, 0.2 mg Se/kg) or a Se‐deficient diet (ED group, 0.008 mg Se/kg) for 45 days. During the trial period of 15–30 days, biological properties such as osmotic fragility, fluidity, phospholipid components of cell membrane, adenosine triphosphatase activities, and antioxidant function of erythrocytes in broilers were examined. Moreover, the messenger RNA (mRNA) expressions of genes associated with inflammation, glycometabolism, and avian uncoupling protein (avUCP) were detected. We found that compared with the C group, hemolysis rate, degree of polarization, and microviscosity of erythrocytes were increased in broilers of the ED group. The composition of erythrocyte membrane lipids was changed. Meanwhile, the antioxidant function of erythrocytes was weakened and mRNA levels of inflammatory genes were stimulated by Se deficiency (p < 0.05). In addition, mRNA expressions of rate‐limiting enzymes in glycometabolism were effected and avUCP mRNA level was downregulated (p < 0.05) in the ED group. It has been concluded from the results that oxidative stress, inflammatory response, and glycometabolism disorder lead to erythrocyte hemolysis by changing the structure and function of erythrocyte membrane in ED broilers suffered from Se deficiency.

Hematological effects of ultrafine carbon black on red blood cells and hemoglobin

“…hRBCs are widely used as a model to explore the oxidative damage. The impaired antioxidative system of hRBCs causes membrane damage due to the accumulation of free radicals and lipid peroxides . The protective mechanisms of the complete defense system for active oxygen scavenging in higher animals mainly include antioxidants and anti‐oxidases .…”

“…The impaired antioxidative system of hRBCs causes membrane damage due to the accumulation of free radicals and lipid peroxides. [34] The protective mechanisms of the complete defense system for active oxygen scavenging in higher animals mainly include antioxidants and anti-oxidases. [35] The anti-oxidases include SOD, GSH-Px, and CAT; [36] these antioxidant enzymes not only synergistically prevent the damage of ROS, but also protect each other.…”

Influence of bacterial organic selenium on blood parameters, immune response, selenium retention and intestinal morphology of broiler chickens

“…Hematological indices are good indicators of the animals' physiological status and have a positive correlation with the animals' nutritional status [13]. Selenium deficiency is associated with high generation of reactive oxygen species and exposure of erythrocytes to high degrees of oxidative stress [14]. Glutathione peroxidase is an enzyme that plays a major role in protection of erythrocytes and hemoglobin in erythrocytes against free radicals and oxidative stress.…”

Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

“…Hematological indices are good indicators of the animals' physiological status and have a positive correlation with the animals' nutritional status [13]. Selenium de ciency is associated with high generation of reactive oxygen species and exposure of erythrocytes to high degrees of oxidative stress [14]. Glutathione peroxidase is an enzyme that plays a major role in protection of erythrocytes and hemoglobin in erythrocytes against free radicals and oxidative stress.…”