Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Shcherbitskaia, Anastasiia D.; Komarova, Evgenia M.; Milyutina, Yu. P.; Ishchuk, Maria A.; Sagurova, Yanina M.; Safaryan, Galina K.; Lesik, Elena A.; Gzgzyan, Alexander M.; Bespalova, Olesya N.; Kogan, Igor Yu.

doi:10.3390/ijms231710060

Cited by 19 publications

(20 citation statements)

References 55 publications

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“…Sperm DNA fragmentation is often present in abnormal semen results, and has a good potential for prediction of aberrant fertilization in assisted reproductive technology ( 27 , 28 ). Similarly, increased DNA fragmentation was also observed in decreased semen quality in men with COVID-19, together with an imbalance of oxidation and anti-oxidation ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

Exploration of the common genetic landscape of COVID-19 and male infertility

et al. 2023

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BackgroundCOVID-19 has spread widely across continents since 2019, causing serious damage to human health. Accumulative research uncovered that SARS-CoV-2 poses a great threat to male fertility, and male infertility (MI) is a common comorbidity for the COVID-19 pandemic. The aim of the study was to explore the cross-talk molecular mechanisms between COVID-19 and MI.Materials and methodsA total of four transcriptome data regarding COVID-19 and MI were downloaded from the Gene Expression Omnibus (GEO) repository, and were divided for two purposes (initial analysis and external validation). Differentially expressed genes (DEGs) analysis, GO and pathway annotation, protein-protein interaction (PPI) network, connectivity ranking, ROC analysis, immune infiltration, and translational and post-translational interaction were performed to gain hub COVID-19-related DEGs (CORGs). Moreover, we recorded medical information of COVID-19 patients with MI and matched healthy controls, and harvested their sperm samples in the university hospital. Expressions of hub CORGs were detected through the qRT-PCR technique.ResultsWe identified 460 overlapped CORGs in both the COVID-19 DEGs and MI DEGs. CORGs were significantly enriched in DNA damage and repair-associated, cell cycle-associated, ubiquitination-associated, and coronavirus-associated signaling. Module assessment of PPI network revealed that enriched GO functions were closely related to cell cycle and DNA metabolism processes. Pharmacologic agent prediction displayed protein-drug interactions of ascorbic acid, biotin, caffeine, and L-cysteine with CORGs. After connectivity ranking and external validation, three hub CORGs (ENTPD6, CIB1, and EIF3B) showed good diagnostic performance (area under the curve > 0.75). Subsequently, three types of immune cells (CD8+ T cells, monocytes, and macrophages M0) were dominantly enriched, and 24 transcription factor-CORGs interactions and 13 miRNA-CORGs interactions were constructed in the network. Finally, qRT-PCR analysis confirmed that there were significant differences in the expression of hub CORGs (CIB1 and EIF3B) between the patient and control groups.ConclusionThe present study identified and validated hub CORGs in COVID-19 and MI, and systematically explored molecular interactions and regulatory features in various biological processes. Our data provide new insights into the novel biomarkers and potential therapeutic targets of COVID-19-associated MI.

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Section: Discussionmentioning

confidence: 99%

Exploration of the common genetic landscape of COVID-19 and male infertility

et al. 2023

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“…Следует также отметить, что системные воспалительные процессы могут влиять на уровень цинка в спермоплазме. Например, после COVID-19 в отдельных исследованиях отмечалось снижение уровня цинка в спермоплазме (p ≤0,05; n=17) на фоне фрагментации ДНК сперматозоидов 15% и выше [43]. В проведенном нами исследовании (n=144) после бессимптомных и легких форм COVID-19 достоверного изменения уровня цинка в спермоплазме не отмечено [44].…”

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Correlation of the zinc level in the spermoplasm with the fertility characteristics of human ejaculate

Lutsky

Lutskaya

Mahmudov

et al. 2023

Journal of Clinical Practice

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Background: Zinc is essential for the normal functioning of the male reproductive system. The data on the diagnostic value of the determination of zinc in human spermoplasm and the relationship of its level with the main parameters of sperm fertility are contradictory. Aims: Study of correlations of zinc level in spermoplasm with spermogram characteristics. Methods: Sperm of men of reproductive age (n=486, average age 33.073.03 years) was studied. In addition to the standard spermogram, MAR tests (IgA, IgG and IgM) were performed in the sperm samples, the degree of fragmentation of sperm DNA was assessed, the interaction of sperm with hyaluronic acid was assessed, acrosine activity, neutral alpha-glucosidase activity was determined, citric acid, fructose and glycodelin levels were determined, the level of reactive oxygen species was studied. Determination of the zinc level in the spermoplasm was carried out by a standard spectrophotometric method with 5-Br-PAPS chromogen. Pearson's formula was used for correlation analysis. The study was conducted from 2018 to May 2022, once. Results: A significant negative correlation of the zinc level in the spermoplasm with the age of men was revealed (r=-0.1625, p0.001). The level of zinc in the spermoplasm was weakly negatively correlated with the dilution time and with the viscosity of the sperm. The positive correlation was with the number of spermatozoa (r=0.1345, p0.01) and their mobility (r=0.3825, p0.00001). The level of zinc in the spermoplasm negatively correlates with the degree of fragmentation of sperm DNA and with the amount of reactive oxygen species, and with the test for binding of sperm with hyaluronic acid positively. Conclusions: The level of zinc in the spermoplasm significantly correlates with a number of physiological and biochemical characteristics of sperm. The data obtained allow us to recommend the determination of zinc in the sperm plasma not only to assess the functional activity of the prostate gland, but also to diagnose the fertility of the ejaculate, as well as to optimize therapy with zinc-containing drugs and improve control over the effectiveness of the treatment.

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“…The results of semen analysis for a median observation time of 56 and 78 days also suggest that COVID-19 has adverse but potentially reversible consequences on sperm quality 29 . Additional analyses of oxidative stress markers and sperm DNA for semen samples suggest that increased DNA fragmentation and decreased semen quality in men may be the result of an imbalance between semen precursors and antioxidant components after COVID-19 30 .…”

Section: Discussionmentioning

confidence: 99%

Evaluating male sexual function and reproductive health during Omicron outbreak in China

Xiao,

Liu,

Matsika

et al. 2023

Preprint

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Background There are currently no studies exploring omicron infection and male sexual function and semen quality. Our aim was to estimate changes in sexual function and semen quality in men recovering from infection since the Omicron pandemic started in China in late 2022. Methods We collected 1540 questionnaires and assessed male function before infection and acute phase after infection by using International Index of Erectile Function-5, Premature Ejaculation Diagnostic Tool, and Arizona Sexual Experience Scale. We also collected the before and after semen analysis results of 247 male patients. Results During the acute phase after infection, the proportion of erectile dysfunction was significantly higher than before infection, but ejaculatory function was not significantly altered; In addition, semen analysis showed significant difference in semen concentration, semen activity and PR a + b sperm forward movement after infection compared to pre-infection. Conclusions Omicron can affect erectile function as well as sexual experience in male patients in the acute phase. The risk factors for decreased sexual function due to Omicron included body temperature and anxiety during infection. Trial registration: The study was registered with ClinicalTrials.gov and approved by the Ethics Committee of Xiangya Medical College.

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Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Cited by 19 publications

References 55 publications

Exploration of the common genetic landscape of COVID-19 and male infertility

Exploration of the common genetic landscape of COVID-19 and male infertility

Correlation of the zinc level in the spermoplasm with the fertility characteristics of human ejaculate

Evaluating male sexual function and reproductive health during Omicron outbreak in China

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