Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis

Elmehy, Dalia A.; Ismail, Howaida I. H.; Soliman, Nema A.; Amer, Basma; Elkaliny, Heba H; El-Ebiary, Ahmad A.; Gamea, Ghada A.

doi:10.1016/j.actatropica.2020.105760

Cited by 10 publications

(7 citation statements)

References 67 publications

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“…As a result of the diminished number of both worms and larvae, restoration of the normal appearance of host cells was noted accompanied by an obvious diminishment in inflammatory cells (Attia et al, 2015;Abou Rayia et al, 2017;Elgendy et al, 2020) particularly in combined ABZ-MQ treated group. The confirmed parasiticidal effects of MQ in this study may be due to its involvement in ROS generation (Elmehy et al,2021) in addition to its anti-angiogenic and apoptotic properties against the parasites in hosted tissues (Kamili et al, 2017).…”

Section: Discussionsupporting

confidence: 65%

“…Furthermore, it has anti-angiogenic properties against cancer cells (Kamili et al, 2017) raising its activity of decreasing the muscle larval counts by making the skeletal muscle cells less hospitable to Trichinella larvae, depriving the larvae of their nourishment with the accumulation of their wastes triggering their death. The effectiveness of MQ against T.spiralis infection is related to its oxidative, apoptotic, and inflammatory properties (Elmehy et al, 2021). Interestingly, the combined treatment shows a tendency to have better therapeutic efficacy than the single drugs.…”

Section: Discussionmentioning

confidence: 99%

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Therapeutic efficacy of Albendazole and Mefloquine alone or in combination against early and late stages of Trichinella spiralis infection in mice

Fahmy

Diab

2021

Helminthologia

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Summary This study aimed to determine the effectiveness of mefl oquine alone or combined with albendazole in reduced doses against T. spiralis infection. One hundred and twenty albino mice were orally infected with 200 T. spiralis larvae/mouse. Drugs were administered during the enteral phase on days 1 to 3 and on the chronic phase on days 35 to 37 post-infection, and mice were sacrificed, respectively, at days 7 or 48 post-infection to count mature intestinal worms or encysted muscle larvae. The effect of the treatment on the histology of the target organs of each phase, intestine and diaphragm, was also evaluated. A signifi cant decrease in intestinal worms was found in all treated groups relative to the untreated control group at a peak of 93.7% in the combination albendazole-mefl oquine group. Results in all treated groups demonstrated a signifi cant decrease in muscle larvae relative to untreated control groups, achieving 86.2 % in the combined albendazole-mefl oquine group. There was a marked improvement in the intestinal and muscular architecture in all treated groups compared to the non-treated control group. Notably, the albendazole-mefl oquine group showed an almost complete recovery. The combined albendazole-mefl oquine low dose regimen had the highest effect on reducing parasite burden and restoring normal histological architecture.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Therapeutic efficacy of Albendazole and Mefloquine alone or in combination against early and late stages of Trichinella spiralis infection in mice

Fahmy

Diab

2021

Helminthologia

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“…Next, the larvae convey through the circulatory system into the skeletal muscle cells ISSN (Print) 2786-0272 ISSN (Online) 2786-0280 NVVJ. 2 (1), 2022 (Othman et al, 2016), where they transform muscle cells into encapsulated nurse cells that remain from months to years (Elmehy et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

Swilam

Nasreldin²,

Abd-Elrahman³

et al. 2022

New Valley Veterinary Journal

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Trichinella spiralis (T. spiralis) is one of the parasitic nematodes of a highly zoonotic importance. It infects various vertebrates, including human. In this study eighty (n =80) Swiss albino mice were experimentally infected by T. spiralis to assess the nematocidal activity of tannic acid (TA) and albendazole (ABZ) individually or in combination during the intestinal phase of T. spiralis. Mice were divided into 10 equal groups 8 mice for each, (control): noninfected but received saline, (ABZ): non-infected and treated with ABZ 50 mg/kg, (TA1000): non-infected and treated with TA1000 µg/ml, (TA2000): non-infected and treated with TA2000 µg/ml, (ABZ+TA2000): non-infected and treated with ABZ 50 mg/kg + TA2000 µg/ml , (TS): infected non treated, (TS+ABZ) group: infected and treated with ABZ 50mg/kg, (TS+TA1000): infected and treated with TA1000 µg/ml, (TS+TA2000): infected and treated with TA2000 µg/ml and (TS+ABZ+TA2000): infected and treated with ABZ 50mg/kg + TA2000 µg/ml. Mice were sacrificed at 7 th day post infection. Results demonstrated significant reductions in the adult worm count in TS+TA1000, TS+ABZ, TS+ TA2000 and TS+ABZ+TA2000 with efficacies of 91%, 92%, 92% & 99% respectively. Results were confirmed by histopathological examination of the targeted organs. The degree of necrosis was decreased in treated groups, associated with reduction in the percentage of eosinophils. Elevated concentrations of serum ALT, AST, and urea in the infected group were decreased in treated groups, while albumin and glucose were increased.It was concluded that the combined albendazole-tannic acid therapy had the highest effect on reducing parasite burden and restoring normal histological architecture.

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“…For the muscular phase, mice of subgroups (B) were sacrificed at the 45th dpi (Elmehy et al . 2021).…”

Section: Methodsmentioning

confidence: 99%

Antihelminthic and antiangiogenic effects of zinc oxide nanoparticles on intestinal and muscular phases of trichinellosis

ASHOUSH

Soliman

2023

J. Helminthol.

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Trichinellosis is a worldwide zoonotic disease affecting a wide range of mammals, including humans. It has intestinal and muscular phases. The current work was done to experimentally evaluate the efficacy of zinc oxide nanoparticles (ZnO NPs) and their combination with albendazole on intestinal and muscular stages of Trichinella spiralis (T. spiralis) infection. We had five main groups of mice: Group 1, non-infected control; Group 2, infected control; Group 3, infected and treated with albendazole; Group 4, infected and treated with ZnO NPs; and Group 5, infected and treated with albendazole and ZnO NPs. Each group was divided into two subgroups (A for the intestinal phase and B for the muscular phase). Drug effects were evaluated by parasitological, histopathological, and biochemical studies, including oxidant/antioxidant analysis and vascular endothelial growth factor (VEGF) gene expression in muscle tissue by quantitative real-time PCR. ZnO NPs resulted in a significant reduction of both intestinal and muscular phases of T. spiralis. Their combination with albendazole resulted in the complete eradication of adult worms and the maximum reduction of larval deposition in muscle tissue. Additionally, the treatment showed improvement in T. spiralis-induced pathological changes and oxidative stress status. Moreover, a significant decrease in VEGF gene expression was detected in the treated groups when compared with the infected control. In conclusion, ZnO NPs presented an antihelminthic effect against both adult and larval stages of T. spiralis. In addition, it enhanced antioxidant status and suppressed angiogenesis in muscle.

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Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis

Cited by 10 publications

References 67 publications

Therapeutic efficacy of Albendazole and Mefloquine alone or in combination against early and late stages of Trichinella spiralis infection in mice

Therapeutic efficacy of Albendazole and Mefloquine alone or in combination against early and late stages of Trichinella spiralis infection in mice

Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

Antihelminthic and antiangiogenic effects of zinc oxide nanoparticles on intestinal and muscular phases of trichinellosis

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