Oxidative stress, senescence and Mediterranean diet effects on osteoarthritis

Ammendola, Sergio; d’Abusco, Anna Scotto

doi:10.1016/b978-0-12-818698-5.00007-9

Cited by 5 publications

(4 citation statements)

References 50 publications

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“…Therefore, these elements were not shown and not taken for further consideration. It should be mentioned that tea, which is known to contain antioxidants (e.g., polyphenols), also contains pro-oxidant compounds that are capable of causing oxidative reactions, leading to the unfolding of proteins and DNA damage, e.g., iron ions, copper ions and heavy metals [53], which is very important in the context of the presented research. Iron is an important microelement which is responsible for a number of metabolic processes in the human body.…”

Section: Resultsmentioning

confidence: 99%

Do You Know What You Drink? Comparative Research on the Contents of Radioisotopes and Heavy Metals in Different Types of Tea from Various Parts of the World

Grządka,

Bastrzyk,

Orzeł

et al. 2024

Foods

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The aim of this study was to assess the potential health risks of radioactive elements and heavy metals ingested through the consumption of various types of tea imported to the Polish market (black, green, red, oolong and white). The concentrations [Bq/kg] of radionuclides (40K, 137Cs, 226Ra, 210Pb and 228Th) in tea leaves before and after brewing were measured using γ-ray spectrometry with high-purity germanium (HPGe). The concentrations [mg/kg] of the studied elements (Fe, Cr, Cu, Mo, Al, Mn, Ni, P, V, Cd and Pb) were determined using a microwave-induced plasma optical emission spectrometer (MIP-OES). The results presented here will help to expand the database of heavy metals and radioactivity in teas. With regard to the potential health risk, the percentage of leaching of individual elements in different types of tea infusions was determined, and the assessment of the consumption risk was estimated. Since the calculated exposure factors, namely the HQ (Hazard Quotient) and THQ (Target Hazard Quotient), do not exceed critical levels, teas can still be considered health-beneficial products (most of the radionuclides as well as elements remain in the leaves (65–80%) after brewing).

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Section: Resultsmentioning

confidence: 99%

Do You Know What You Drink? Comparative Research on the Contents of Radioisotopes and Heavy Metals in Different Types of Tea from Various Parts of the World

Grządka,

Bastrzyk,

Orzeł

et al. 2024

Foods

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“…Next chondrocytes are enzymatically isolated from the cartilage and expanded in vitro in monolayer for 4 to 6 weeks until a sufficient number of cells are available to be implanted into the defect site. Given that chondrocytes represent only 2% of the AC volume, the expansion procedure is essential in order to augment the limited number of isolated chondrocytes. , However, the main disadvantage of this procedure is that chondrocytes when expanded in vitro in 2D tend to lose their original phenotype and acquire a more fibroblastic appearance . The dedifferentiation process is characterized by morphological changes as well as shifts in protein synthesis and gene expression, decreased cell proliferation, increased apoptosis, and cell senescence .…”

Section: Introductionmentioning

confidence: 99%

Collagen and Alginate Hydrogels Support Chondrocytes Redifferentiation In Vitro without Supplementation of Exogenous Growth Factors

Roncada,

Blunn,

Roldo

2024

ACS Omega

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Focal cartilage defects are a prevalent knee problem affecting people of all ages. Articular cartilage (AC) possesses limited healing potential, and osteochondral defects can lead to pain and long-term complications such as osteoarthritis. Autologous chondrocyte implantation (ACI) has been a successful surgical approach for repairing osteochondral defects over the past two decades. However, a major drawback of ACI is the dedifferentiation of chondrocytes during their in vitro expansion. In this study, we isolated ovine chondrocytes and cultured them in a two-dimensional environment for ACI procedures. We hypothesized that 3D scaffolds would support the cells' redifferentiation without the need for growth factors so we encapsulated them into soft collagen and alginate (col/alg) hydrogels. Chondrocytes embedded into the hydrogels were viable and proliferated. After 7 days, they regained their original rounded morphology (aspect ratio 1.08) and started to aggregate. Gene expression studies showed an upregulation of COL2A1, FOXO3A, FOXO1, ACAN, and COL6A1 (37, 1.13, 22, 1123, and 1.08-fold change expression, respectively) as early as day one. At 21 days, chondrocytes had extensively colonized the hydrogel, forming large cell clusters. They started to replace the degrading scaffold by depositing collagen II and aggrecan, but with limited collagen type I deposition. This approach allows us to overcome the limitations of current approaches such as the dedifferentiation occurring in 2D in vitro expansion and the necrotic formation in spheroids. Further studies are warranted to assess long-term ECM deposition and integration with native cartilage. Though limitations exist, this study suggests a promising avenue for cartilage repair with col/alg hydrogel scaffolds.

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“…It has been discovered that some natural molecules are scavengers of reactive oxygen species (ROS) or reactive nitrogen species (RNS). Glucosamine, polyphenols, vitamin E (tocopherol), and vitamin C (ascorbic acid) are a few of the commonly used molecules to trigger the enzyme-mediated antioxidant response (Hamidi et al, 2019;Binsi and Zynudheen, 2019;Ammendola and D'Abusco, 2020). Pro-oxidants are substances that create reactive oxygen species (ROS) or inhibit antioxidant systems, causing oxidative stress (Rahal et al, 2014;Zhang et al, 2018;Alfei et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…It has also been found that some metals are beneficial in antioxidant system. Numerous biomolecules, such as enzymes, as well as a few microelements (such as Cu, Zn, Mn, and Se) make up the antioxidant system in living organisms (Niklowitz et al, 2005;Aycicek et al, 2006;Hamidi et al, 2019;Binsi and Zynudheen, 2019;Ammendola and D'Abusco, 2020).…”

Section: Introductionmentioning

confidence: 99%

Elemental and In vitro Antioxidant Studies of Some Bracharia species and Milk from Bowen University Dairy Farm

Ogunlakin,

Odugbemi,

Omolekan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study investigated the constituent elements present in three Bracharia grasses and milk samples from the Bowen Dairy cows. Bracharia grasses and milk samples were subjected to Atomic Absorption Spectroscopy (AAS) for determination of K, Ca, Na, Mn, Fe, Cd and Cu, and in vitro antioxidant activities. Levels of K, Ca and Na in both fresh and sterilized milk were also comparable to the RDA. No levels of Cd and Cu were determined all samples. The level of Mn in fresh milk was 0.80 ± 0003 ppm, but was not determined in sterilized milk. The AAS result shows that the levels of K, Ca and Na in Brachiaria sabia methanol extract (BSME), Bracharia gayana methanol extract (BGME) and Bracharia maradu methanol extract (BMME) were lower than the RDA value. However, Cd and Cu were not determined in the three samples, except in BMME. The fresh and sterilized milk samples had better antioxidant potentials compared to quercetin standard. This study indicates that the Bracharia grasses and milk samples from Bowen University Dairy Farm are rich in K and Fe. In addition, both fresh and sterilized milk samples possess strong in vitro antioxidant capacity.

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Oxidative stress, senescence and Mediterranean diet effects on osteoarthritis

Cited by 5 publications

References 50 publications

Do You Know What You Drink? Comparative Research on the Contents of Radioisotopes and Heavy Metals in Different Types of Tea from Various Parts of the World

Do You Know What You Drink? Comparative Research on the Contents of Radioisotopes and Heavy Metals in Different Types of Tea from Various Parts of the World

Collagen and Alginate Hydrogels Support Chondrocytes Redifferentiation In Vitro without Supplementation of Exogenous Growth Factors

Elemental and In vitro Antioxidant Studies of Some Bracharia species and Milk from Bowen University Dairy Farm

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