Oxidized alginate beads for tunable release of osteogenically potent mesenchymal stromal cells

Xiang, Gao; Lippens, Evi; Hafeez, Shahzad; Duda, Georg N.; Geißler, Sven; Qazi, Taimoor H.

doi:10.1016/j.msec.2019.109911

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…Due to their faster degradation rate and higher content of reactive groups compared to native alginate, hydrogels based on oxidized alginate (OA) are used widely as biodegradable materials for tissue engineering applications. OA-based hydrogels are used in tissue engineering of bone, cartilage, blood vessels, cornea, and other soft tissues [ 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 ].…”

Section: Chemical Properties Of Alginates and Modification Methodsmentioning

confidence: 99%

Modification of Alginates to Modulate Their Physic-Chemical Properties and Obtain Biomaterials with Different Functional Properties

et al. 2021

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Modified alginates have a wide range of applications, including in the manufacture of dressings and scaffolds used for regenerative medicine, in systems for selective drug delivery, and as hydrogel materials. This literature review discusses the methods used to modify alginates and obtain materials with new or improved functional properties. It discusses the diverse biological and functional activity of alginates. It presents methods of modification that utilize both natural and synthetic peptides, and describes their influence on the biological properties of the alginates. The success of functionalization depends on the reaction conditions being sufficient to guarantee the desired transformations and provide modified alginates with new desirable properties, but mild enough to prevent degradation of the alginates. This review is a literature description of efficient methods of alginate functionalization using biologically active ligands. Particular attention was paid to methods of alginate functionalization with peptides, because the combination of the properties of alginates and peptides leads to the obtaining of conjugates with properties resulting from both components as well as a completely new, different functionality.

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Section: Chemical Properties Of Alginates and Modification Methodsmentioning

confidence: 99%

Modification of Alginates to Modulate Their Physic-Chemical Properties and Obtain Biomaterials with Different Functional Properties

et al. 2021

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“…Therefore, many scholars have proposed tuning the degradation rate of alginate-based hydrogels by oxidizing alginate. 21,22 In recent years, many multifunctional OA-based hydrogels synthesized by photocross-linking, 11,23 Schiff base cross-linking, 22,24 or ionic crosslinking 25 have been used for bone or soft tissue regeneration, cartilage repair, drug or cell delivery vectors, or as 3D-printed bioink materials or membrane and coating materials because of their excellent physicochemical and biological performances and biodegradability and have achieved satisfactory results. 15,16 Among them, photocross-linked OA hydrogels have attracted great interest in regenerative medicine because they possess the vital advantage of easily controlled degradation kinetics, in addition to many outstanding advantages, similar to the photocross-linked alginate hydrogels mentioned above.…”

Section: Introductionmentioning

confidence: 99%

“…In view of this, the regulation of the degradation kinetics of alginate hydrogels by focusing on oxidized alginate (OA) has attracted continuously increasing attention for numerous biomedical applications. , Alginate can be oxidized by sodium periodate, causing cleavage of the carbon–carbon bond and forming two aldehyde groups at the second and third carbon positions of the repetitive unit of the alginate chain, and the degradation rate of OA-based hydrogels is significantly increased compared with that of unoxidized alginate hydrogels. Therefore, many scholars have proposed tuning the degradation rate of alginate-based hydrogels by oxidizing alginate. , In recent years, many multifunctional OA-based hydrogels synthesized by photocross-linking, , Schiff base cross-linking, , or ionic cross-linking have been used for bone or soft tissue regeneration, cartilage repair, drug or cell delivery vectors, or as 3D-printed bioink materials or membrane and coating materials because of their excellent physicochemical and biological performances and biodegradability and have achieved satisfactory results. , Among them, photocross-linked OA hydrogels have attracted great interest in regenerative medicine because they possess the vital advantage of easily controlled degradation kinetics, in addition to many outstanding advantages, similar to the photocross-linked alginate hydrogels mentioned above. Surprisingly, the lack of design of hydrogels with suitable degradation rates to meet the speed of regeneration of tissues has severely hampered their further development.…”

Section: Introductionmentioning

confidence: 99%

Degradation-Kinetics-Controllable and Tissue-Regeneration-Matchable Photocross-linked Alginate Hydrogels for Bone Repair

Zhao

Wang

Cheng

et al. 2022

ACS Appl. Mater. Interfaces

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Photocross-linked alginate hydrogels, due to their biodegradability, biocompatibility, strong control for gelling kinetics in space and time, and admirable adaptability for in situ polymerization with a minimally invasive approach in surgical procedures, have created great expectations in bone regeneration. However, hydrogels with suitable degradation kinetics that can match the tissue regeneration process have not been designed, which limits their further application in bone tissue engineering. Herein, we finely developed an oxidation strategy for alginate to obtain hydrogels with more suitable degradation rates and comprehensively explored their physical and biological performances in vitro and in vivo to further advance the clinical application for the hydrogels in bone repair. The physical properties of the gels can be tuned via tailoring the degree of alginate oxidation. In particular, in vivo degradation studies showed that the degradation rates of the gels were significantly increased by oxidizing alginate. The activity, proliferation, initial adhesion, and osteogenic differentiation of rat and rabbit bone marrow stromal cells (BMSCs) cultured with/in the hydrogels were explored, and the results demonstrated that the gels possessed excellent biocompatibility and that the encapsulated BMSCs were capable of osteogenic differentiation. Furthermore, in vivo implantation of rabbit BMSC-loaded gels into tibial plateau defects of rabbits demonstrated the feasibility of hydrogels with appropriate degradation rates for bone repair. This study indicated that hydrogels with increasingly controllable and matchable degradation kinetics and satisfactory bioproperties demonstrate great clinical potential in bone tissue engineering and regenerative medicine and could also provide references for drug/growth-factor delivery therapeutic strategies for diseases requiring specific drug/growth-factor durations of action.

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“…[4,5] However, traditionally used bulk hydrogels are often rigid and non-porous, which prevents host cells from invading. [6] Strategies to overcome this include incorporating enzymatically or hydrolytically degradable sites within the hydrogel, [7,8] fabricating hydrogels from decellularized extracellular matrices (dECMs) with inherent degradability, [9] or combining with fast-degrading or dissolving porogens to introduce porosity over time. [10] Despite improvements, degradation of synthetic hydrogels and the inclusion of poragens are associated with weakening of mechanical properties and may additionally compromise structural features that provide instructive physical signals to host cells, while batch inconsistencies and limited ability to manipulate properties affect utility of natural dECMs.…”

Section: Main Textmentioning

confidence: 99%

Anisotropic Rod-Shaped Particles Influence Injectable Granular Hydrogel Properties and Cell Invasion

Qazi

Muir

et al. 2021

Preprint

Self Cite

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Granular hydrogels have emerged as a new class of injectable and porous biomaterials that improve integration with host tissue when compared to solid hydrogels. Granular hydrogels are typically prepared using spherical particles and this study considers whether particle shape (i.e., isotropic spheres versus anisotropic rods) influences granular hydrogel properties and cellular invasion. Simulations predict that anisotropic rods influence pore shape and interconnectivity, as well as bead transport through granular assemblies. Photocrosslinkable norbornene- modified hyaluronic acid is used to produce spherical and rod-shaped particles using microfluidic droplet generators and formed into shear-thinning and self-healing granular hydrogels at low and high particle packing. Rod-shaped particles form granular hydrogels that have anisotropic and interconnected pores, with pore number and size, storage moduli, and extrusion forces influenced by particle shape and packing. Robust in vitro sprouting of endothelial cells from embedded cellular spheroids is observed with rod-shaped particles, including higher sprouting densities and sprout lengths when compared to hydrogels with spherical particles. Cellular invasion into granular hydrogels when injected subcutaneously in vivo is significantly greater with rod-shaped particles, whereas a gradient of cellularity is observed with spherical particles. Overall, this work demonstrates potentially superior functional properties of granular hydrogels with rod-shaped particles for tissue repair.

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Oxidized alginate beads for tunable release of osteogenically potent mesenchymal stromal cells

Cited by 13 publications

References 38 publications

Modification of Alginates to Modulate Their Physic-Chemical Properties and Obtain Biomaterials with Different Functional Properties

Modification of Alginates to Modulate Their Physic-Chemical Properties and Obtain Biomaterials with Different Functional Properties

Degradation-Kinetics-Controllable and Tissue-Regeneration-Matchable Photocross-linked Alginate Hydrogels for Bone Repair

Anisotropic Rod-Shaped Particles Influence Injectable Granular Hydrogel Properties and Cell Invasion

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