Oxidized Chitin Nanocrystals Greatly Strengthen the Stability of Resveratrol-Loaded Gliadin Nanoparticles

Abstract

Resveratrol (RES) is a natural polyphenol with a variety of health beneficial properties, but its application is greatly limited due to low aqueous solubility and poor bioavailability. This study aims to address these issues via gliadin nanoparticles stabilized with oxidized chitin nanocrystals (O-ChNCs) as a delivery system for RES. RES-loaded gliadin nanoparticles (GRNPs) were fabricated by an antisolvent method, and their formation mechanism was elucidated using zeta-potential, FTIR, XRD, and TEM. Furthermore, the effect of O-ChNCs on the colloidal stability and bioactiveness of GRNPs was discussed. The results demonstrate that O-ChNCs are adsorbed onto the surface of GRNPs through hydrogen bonding and electrostatic interactions, leading to the enhanced absolute potential and the improved hydrophobicity of the particles, which in turn facilitates the stability of the GRNPs. Furthermore, the changes in the release profile and antioxidant activity of RES in the simulated gastric and intestinal tracts indicate that the adsorption of O-ChNCs not only delays the release of RES but also has a protective effect on the antioxidant capacity of RES. This study provides significant implications for developing stable gliadin nanoparticles as delivery vehicles for bioactive substances.

Comparison of Alternative Protein Hydrogels for Delivering Myricetin: Interaction Mechanism and Stability Evaluation

“…For instance, the hydrophobic bond peaks of WPI and EDP at 2970 and 2923 cm –1 were shifted to 2927 and 2917 cm –1 , which indicated that hydrophobic interactions were presented between Myr and proteins. In addition, Myr showed a broad peak at 3415 cm –1 , corresponding to the O–H stretching vibration . With respect to the TMP–Myr and YP–Myr hydrogels, the characteristic peaks of hydrogen bonding were shifted from 3385 and 3389 cm –1 to 3404 and 3374 cm –1 , respectively.…”

“…In addition, Myr showed a broad peak at 3415 cm −1 , corresponding to the O−H stretching vibration. 22 With respect to the TMP−Myr and YP−Myr hydrogels, the characteristic peaks of hydrogen bonding were shifted from 3385 and 3389 cm −1 to 3404 and 3374 cm −1 , respectively. Similar shift phenomena were also observed in the formation of the SPI− Myr, WPI−Myr, and EDP−Myr hydrogels.…”

“…The interface properties of protein hydrogels and protein–Myr hydrogels were characterized by an SDC-200S contact angle measuring apparatus (China) . The contact angle and surface tension were determined by the geometric angle method and the hanging drop method, respectively.…”

“…The interface properties of protein hydrogels and protein−Myr hydrogels were characterized by an SDC-200S contact angle measuring apparatus (China). 22 The contact angle and surface tension were determined by the geometric angle method and the hanging drop method, respectively. In this experiment, the specific parameters were a temperature of 25 °C, droplet volume of 0.5 μL, and time interval of 0.5 s.…”

Preparation and Characterization of Fish Oil Pickering Emulsions Stabilized by Resveratrol-Loaded Gliadin/Chitin Nanocrystal Composite Nanoparticles

“…GR/OC composite nanoparticles were prepared following a previous study. 17 The resulting composite nanoparticles exhibited the highest encapsulation rate of RES with a ratio of 2 × 10 −3 of RES to gliadin. Additionally, for the OCh-GRNP suspension, the three-phase contact angle approached 90°at a gliadin/O-ChNC mass ratio of 1:1.5, indicating that the nanoparticles were most stable in solution at this ratio.…”