Oxidized fish oil injury stress in Megalobrama amblycephala: Evaluated by growth, intestinal physiology, and transcriptome-based PI3K-Akt/NF-κB/TCR inflammatory signaling

Song, Changyou; Liu, Bo; Xu, Pao; Xie, Jun; Ge, Xianping; Zhou, Qunlan; Sun, Cunxin; Zhang, Huimin; Shan, Fan; Yang, Zhenfei

doi:10.1016/j.fsi.2018.07.049

Cited by 78 publications

(43 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is supported by studies on other fish and crustaceans fed oxidized lipid, such as channel catfish ( Ictalurus punctatus ), red sea bream ( Pagrus major ), Chinese mitten crab ( Eriocheir sinensis ), blunt snout bream ( M . amblycephala ), Japanese sea bass ( Lateolabrax japonicus ) and Pacific white shrimp (Dong et al, 2012; Gao et al, 2013; Song et al, 2018, 2019; Wang et al, 2015; Yang et al, 2015; Yu‐Zhe et al, 2012). Jiang demonstrated that diets containing oxidized silkworm pupae (acid value: 15.36 mg KOH g −1 ) reduced the growth and feed utilization of crucian carp (Jiang, 2010), which has similar results to this study.…”

Section: Discussionmentioning

confidence: 99%

Effects of oxidized silkworm ( Bombyx mori L .) pupae on growth performance, and intestine, liver and muscle histology and function of Gif Tilapia ( Oreochromis niloticus )

Feng

Wang

Huang

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

Silkworm (Bombyx mori L.) pupae are a high‐quality feed ingredient, rich in protein and fat. However, silkworm pupae are prone to oxidative rancidity due to the high content of unsaturated fatty acids, which limits its large‐scale use in feed. A 56‐day feeding study was conducted to determine the effects of oxidized silkworm pupae on growth performance, structure and function of the intestine, liver and muscle in genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Three approximately isonitrogenous (crude protein: 328 g kg−1) and isolipidic (crude lipid: 56 g kg−1) diets were formulated containing 4% fresh silkworm pupae, lightly oxidized silkworm pupae and highly oxidized silkworm pupae, which named FSP, LOSP and HOSP respectively. Three groups of GIFT with an initial weight of (44.22 ± 0.54) g were randomly assigned to each diet. The results showed that the weight gain rate (WGR), specific growth rate and feed intake decreased with increasing silkworm pupae oxidative levels (p < 0.05). Villus height in LOSP and HOSP significantly decreased with increasing silkworm pupae oxidative levels (p < 0.05), while villus number and muscular thickness of foregut were similar among three groups (p > 0.05). Dietary oxidized silkworm pupae significantly reduced the activity of intestinal Na+–K+–ATPase compared with FSP group (p < 0.05), whereas that was similar in LOSP and HOSP groups (p > 0.05). The activities of intestinal protease, lipase and pepsin in HOSP group were significantly reduced compared with FSP group (p < 0.05), whereas those were similar in LOSP and FSP groups (p > 0.05). Hepatocytes with swelling and nuclear migration occurred in the liver of LOSP and HOSP groups. Malondialdehyde (MDA) content of LOSP and HOSP groups in liver was significantly higher than that in FSP group (p < 0.05). Muscular histological examinations indicated that muscle fibre atrophy occurred in HOSP group. The content of crude protein and crude ash in muscle of HOSP group was significantly reduced compared with FSP and LOSP groups (p < 0.05). ∑n‐3PUFA contents in the muscle of HOSP decreased by 37.28% compared with FSP group (p < 0.05). To sum up, dietary oxidized silkworm pupae inhibited growth, reduced the gastrointestinal digestive and absorptive capacity and hepatic antioxidant capacity, and the negative impacts were increased with the increasing oxidative degree of silkworm pupae. In addition, highly oxidized silkworm pupae have a destructive effect on the histological structure of the foregut, liver and muscle.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of oxidized silkworm ( Bombyx mori L .) pupae on growth performance, and intestine, liver and muscle histology and function of Gif Tilapia ( Oreochromis niloticus )

Feng

Wang

Huang

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

show abstract

“…In addition, the downregulation of PP1 (protein phosphatase 1) (Richard et al 2018) in group B is due to the resistance of fish liver against glycogen synthesis. Another proof that shows the significantly changed lipid metabolism under waterless stress can be confirmed by the upregulation of PI3K (phosphatidylinositol 3-kinase) (Song et al 2018) and cAMP-RP19 (cAMP-regulated phosphoprotein 19-like) (Montero et al 2016). CBPs (calciumbinding protein) (Thiruketheeswaran et al 2016) is generally related with glycoprotein folding and protein synthesis, which maintains the protein activity within animal body to guarantee normally stable structure.…”

Section: Response Mechanism Of Signal Transduction Proteins Under Low-temperature and Waterless Stress Conditionsmentioning

confidence: 94%

Response mechanism of ♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus under low-temperature and waterless stresses using TMT proteomic analysis

et al. 2021

View full text Add to dashboard Cite

Abstract♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus, a hybrid grouper created from artificial breeding, has been widely developed over the past decades. However, the study focusing on lukewarm high-protein-content fish species using advanced techniques has rarely been reported. In this work, the TMT (tandem mass tag)-assisted technique was employed to explore its differentially expressed proteins and response mechanisms under low-temperature dormant and waterless stresses. Our findings suggest that 162 and 258 differentially expressed proteins were identified under low-temperature dormant and waterless stresses, respectively. The waterless preservation treatment further identifies 93 differentially expressed proteins. The identified proteins are categorized and found to participate in lipid metabolism, glycometabolism, oxidative stress, immune response, protein and amino acid metabolism, signal transduction, and other functions. Accordingly, the factors that affect the response mechanisms are highlighted to provide new evidences at protein level.

show abstract

“…Although ROS are normally produced during intracellular physiological reactions, excess production accompanied by insu cient elimination results in protein oxidation and lipid peroxidation that cause structural damage to the cells and, in turn, to the intestinal epithelium. Previous studies showed that A. veronii infection led to ROS overproduction [15,[49][50][51]. The degree of protein oxidation and lipid peroxidation can be re ected by the PC and MDA concentrations, respectively [52].…”

Section: Dietary Val Improved Intestinal Antioxidant Capacity Via Nrf2 Signaling Pathway After Infection With a Veroniimentioning

confidence: 99%

“…Intestinal health is dependent on the maintenance of the intestinal structure, and is adversely affected by high levels of reactive oxygen species (ROS) [14]. The overproduction of ROS could lead to damage to the intestines in sh [15]. Fish have both enzymatic and nonenzymatic defense systems used to scavenge excessive ROS [16,17].…”

mentioning

confidence: 99%

Dietary L-Valine Improved Growth, Antioxidant Capacity, and Alleviated Endoplasmic Reticulum Stress-Mediated Apoptosis in the Intestine of Juvenile Largemouth Bass Micropterus Punctulatus After Infection With Aeromonas Veronii

Zhang¹,

Zhang²,

Zhao³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The intestine is the first line of defense against bacteria, toxins and antigens presents in the lumen of the instine. Aeromonas veronii is an opportunistic pathogen that has been related to mortality in fish. However, the protective mechanisms of intestinal damage caused by Aeromonas veronii are poorly defined. Thus, the objetive of this study was to evaluate the influence of dietary valine (Val) on growth, antioxidant capacity, and endoplasmic reticulum stress-mediated apoptosis in intestines of the juvenile largemouth bass Micropterus punctulatus after infection with Aeromonas veronii (A. veronii). Methods: A total of 720 fish (37.99 ± 0.01 g) were randomly allocated to six groups with three replicates each group, with different levels of Val (11.0, 14.0, 17.0, 20.1, 23.1, and 26.1 g/kg diet, respectively) for 77 d, and then challenged with A. veronii. Results: In the present study, we demonstrated that dietary Val: (1) increased the percent weight gain (PWG), specific growth rate (SGR), feed intake (FI), feed efficiency (FE), protein efficiency ratio (PER), protein production value (PPV), lipid production value (LPV), and ash production value (APV) (P< 0.05); (2) improved survival rate (SR) and alleviated intestinal injury; (3) reduced the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and protein carbonyl (PC) (P< 0.05), and improved the intestinal antioxidant capacity by enhancing the mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), and γ-glutamyl cysteine ligase catalytic subunit (GCLC) (P< 0.05); (4) increased nuclear NFE2-related factor 2 (Nrf2) mRNA and protein levels (P< 0.05); (5) reduced intestinal DNA fragments, and down-regulated the mRNA levels of 78 KD glucose regulated protein (GRP78), activating transcription factor 6 (ATF6), inositol requiring enzyme-1 (IRE1), protein kinase RNA-dependent-like endoplasmic reticulum kinase (PERK), eukaryotic initiation factor 2α (eIF2α), activating transcription factor 4 (ATF4), C/EBP homologous protein (CHOP), cysteine-aspartic protease 9 (caspase 9), and cysteine-aspartic protease 3 (caspase 3), while up-regulating that of B-cell lymphoma-2 (Bcl2) (P< 0.05); (6) decreased phosphorylation of PERK (p-PERK) and phosphorylation of eIF2α (p-eIF2α), caspase 9, and caspase 3 protein levels. Conclusion: Dietary optimal Val level enhanced the growth performance and the intestinal antioxidant capacity via the Nrf2 signaling pathway after infection with A. veronii. Furthermore, this study is the first to demonstrate the role of Val in reducing ERS-mediated apoptosis through modulation of the PERK/eIF2α signaling pathway. Finally, Quadratic regression analysis of the SGR indicated that the dietary Val requirement of largemouth bass (37.99-162.92 g) was 21.50 g/kg diet, corresponding to 46.64 g/kg protein.

show abstract

Oxidized fish oil injury stress in Megalobrama amblycephala: Evaluated by growth, intestinal physiology, and transcriptome-based PI3K-Akt/NF-κB/TCR inflammatory signaling

Cited by 78 publications

References 80 publications

Effects of oxidized silkworm ( Bombyx mori L .) pupae on growth performance, and intestine, liver and muscle histology and function of Gif Tilapia ( Oreochromis niloticus )

Effects of oxidized silkworm ( Bombyx mori L .) pupae on growth performance, and intestine, liver and muscle histology and function of Gif Tilapia ( Oreochromis niloticus )

Response mechanism of ♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus under low-temperature and waterless stresses using TMT proteomic analysis

Dietary L-Valine Improved Growth, Antioxidant Capacity, and Alleviated Endoplasmic Reticulum Stress-Mediated Apoptosis in the Intestine of Juvenile Largemouth Bass Micropterus Punctulatus After Infection With Aeromonas Veronii

Contact Info

Product

Resources

About