Oxidizing modification of proteins in case of a burn injury in rats against the topical treatment with new wound healing preparations

Abstract

Burn injuries are a type of traumatic pathology that is characterized by a complex set of polysystemic shifts. The body's response to a burn injury corresponds to the mechanism of the stress response with the development of the adaptation syndrome, resulting in disruption of the normal flow of oxidizing processes [6]. Oxidizing modification of proteins (OMP) is one of the early and most reliable indicators of tissue damage in case of a free radical pathology. These are proteins but not lipids or nucleic acids that are effective traps of the generated reactive oxygen species (ROS) and their oxidizing modification is regarded as one of early and reliable markers of the oxidizing stress [1, 9]. The oxidates of proteins in oxidizing damages in tissues appear earlier and are more stable compared to the products of lipid peroxidation, in particular plasma proteins subjected to oxidizing degradation having quite long half-life. [1] OMP causes changes in physical and chemical properties of the protein molecule: the fragmentation, aggregation and susceptibility to pro-te olysis [2, 8]. As a result, either the formation of products with a high functional activity or either inactivation of zymophores, or modification of protein molecules, that can have a toxic effect and aggravate the pathologic process, occur. The pres ence of ROS in the wound de fect leads to dena tu ra tion of the syn the sized pro tein and in ac ti va tion of fi bro blasts that im pede wound heal ing [9]. The study of the bio chemi cal as pects of the de vel op ment of a burn injury is nec es sary to gen er ally un der stand the mecha nisms of the heal ing pro cess and de velop a strat egy for its treatment and de vel op ment of new wound heal ing medi cines that af fect the ma jor ity of patho logic links of the wound pro cess. In most stud ies, de voted to the mecha nisms of de vel op ment of a burn wound, the con tent of lipid per oxida tion prod ucts is ana lyzed, the con cen tra tion of oxi dized pro teins is vir tu ally paid no at ten tion [6,7]. The aim of this study is to examine the state of oxidizing modification of proteins in the treatment with new wound healing preparations at the model of the third A-degree burn injury in rats. MATERIALS AND METHODS A model of a burn injury was reproduced on 168 mature male albino rats weighing 200-240 g. In animals, under general anesthesia using thiopental (40 mg/kg) on the depilated skin area on the back, retreating from the spine for 1.5 cm, a burn was simulated with an instrument

Biochemical and planimetric investigations of hydrophilic creams containing ceramides or dexpanthenol on the model of chemical burns

“…Apart from reparative properties, pantothenic acid exhibits anti-inflammatory (it takes part in the synthesis of anti-inflammatory hormones) and immunomodulatory (it stimulates antibody production) effects. 5 The main components of ceramides are glycoceramides, cholesterol, and phospholipids. Glycoceramides decrease metabolic cost and loss of structural material for synthesis, renewing the content of endogenous glycoceramides, as well as work within lipid lamellar systems of the intercellular space facilitating restoration of epidermis and reducing dehydration.…”

“…Every year up to 10000 cases of chemical burns are registered in Ukraine and 50-70% patients have no need for operative intervention. [4][5][6] It should be noted that chemical burns commonly occur as a result of chemical ingestion that causes tissue-necrosis (for example, concentrated acids -acetic, sulfuric, hydrochloric and others). Depending on the concentration and exposure time to substance, deep lesions on skin can form as coagulation necrosis that caused by the coagulation of proteins due to quick elim-ination of water from the epidermis.…”

Changes in biomarkers of endothelial function in the blood after intracerebral hemorrhage in rats with type 2 diabetes mellitus

“…The markers of oxidative modification of proteins (aldehydephenylhydrazones and ketonephenylhydrazones) were evaluated spectrophotometrically by the method of B. Halliwell which is based on the reaction of interaction of oxidized amino-acid residues with 2,4-dinitro-phenylhydrazine (2,4-DNPhH) with formation of 2,4-dinitro-phenylhydrazones, which are proportional to the optical density at 270 and 363 nm (Butko et al, 2013). The content of carbonyl derivatives of oxidized proteins was expressed in units of optical density per mg of protein.…”

“…Oxidative modification of proteins (OMP) is one of the early and the most reliable markers of tissue damage in many pathological conditions that involve free radicals, including T2DM. Different tissue proteins but not lipids or nucleic acids are effective traps of the generated ROS that are overproduced or accumulated in oxidative stress (Butko et al, 2013).…”

The Impact of Metformin and Rosuvastatin on the Markers of Oxidative Stress, Glycemic Control, and Lipid Profile in Rats With Streptozotocin-Nicotinamide-Induced Diabetes After Acute Intracerebral Hemorrhage

“…Oxidative modification of proteins (OMP) is one of the early and the most reliable markers of tissue damage in many pathological conditions that involve free radicals, including T2DM. Different tissue proteins but not lipids or nucleic acids are effective traps of the generated reactive oxygen species (ROS) that are overproduced or accumulated in oxidative stress [2]. In addition, the higher levels of glucose and/or lipids in diabetes are responsible for the increased production of highly reactive carbonyl compounds -a condition re-ferred to as «carbonyl stress».…”