Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XFe96 Extracellular Flux Analyzer

Preez, Gerhard Du; Fourie, Hendrika; Daneel, M.; Miller, Hayley Christy; Höss, Sebastian; Ricci, Cristian; Engelbrecht, Gerhard; Zouhar, Miloslav

doi:10.1038/s41598-020-61054-7

Cited by 22 publications

(16 citation statements)

References 38 publications

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“…This showcases how bulk measurements are blind to single animal heterogeneities and that current OCR measuring methods are mostly reporting the middle body region respiration. The latter is important as, being a proxy for metabolic activity, OCR measurements are routinely used to assess toxicity of new substances [ [48] , [49] , [50] ]. If the tested substances have little impact on the middle body respiration but impact other regions, this would be hardly detected by bulk measurements.…”

Section: Resultsmentioning

confidence: 99%

Unveiling the contribution of the reproductive system of individual Caenorhabditis elegans on oxygen consumption by single-point scanning electrochemical microscopy measurements

Santos

Macedo

Kowaltowski

et al. 2021

Analytica Chimica Acta

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Metabolic analysis in animals is usually either evaluated as whole-body measurements or in isolated tissue samples. To reveal tissue specificities in vivo , this study uses scanning electrochemical microscopy (SECM) to provide localized oxygen consumption rates (OCRs) in different regions of single adult Caenorhabditis elegans individuals. This is achieved by measuring the oxygen reduction current at the SECM tip electrode and using a finite element method model of the experiment that defines oxygen concentration and flux at the surface of the organism. SECM mapping measurements uncover a marked heterogeneity of OCR along the worm, with high respiration rates at the reproductive system region. To enable sensitive and quantitative measurements, a self-referencing approach is adopted, whereby the oxygen reduction current at the SECM tip is measured at a selected point on the worm and in bulk solution (calibration). Using genetic and pharmacological approaches, our SECM measurements indicate that viable eggs in the reproductive system are the main contributors in the total oxygen consumption of adult Caenorhabditis elegans . The finding that large regional differences in OCR exist within the animal provides a new understanding of oxygen consumption and metabolic measurements, paving the way for tissue-specific metabolic analyses and toxicity evaluation within single organisms.

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Section: Resultsmentioning

confidence: 99%

Unveiling the contribution of the reproductive system of individual Caenorhabditis elegans on oxygen consumption by single-point scanning electrochemical microscopy measurements

Santos

Macedo

Kowaltowski

et al. 2021

Analytica Chimica Acta

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“…The rate of oxygen consumption is a vital marker indicating cellular function under normal or metabolically challenged conditions, including a fundamental indicator of mitochondrial function, reflecting on reactive oxygen species (ROS) production and metabolic activity during lifetime under normal or metabolically challenged conditions. Preez developed a novel method to measure the oxygen consumption rate of C. elegans and showed that the oxygen consumption rate is used as a promising functional measurement of toxicity (Preez et al, 2020). Respiration of C. elegans has also been used, although infrequently, to study the effect of toxicant exposure (Schouest et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Genistein and Daidzein effects on the physiological indices of Soybean Cyst Nematodes

Yuan

Sikandar

et al. 2022

Sci. agric. (Piracicaba, Braz.)

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Soybean cyst nematode (SCN, Heterodera glycines) is a chief plant-parasitic nematode of soybean. Application of synthetic chemical nematicides poses negative side effects to human health and the environment. Therefore, the search for a safe and effective approach is more relevant. This study evaluated the effects of Genistein and Daidzein on the physiological index of soybean cyst nematodes, individual morphology, reversal frequency, respiration, and body fluid leaking of second-stage juveniles (J2s) of soybean cyst nematode. The results showed that body length of J2s decreased, while stylet and tail transparent area elongated. Additionally, after the treatment, the body became hollow and shrunken, the J2s stiffened, whereas the reversal frequency decreased dramatically after 24 h of treatment. Moreover, the body fluid leakage was intensified and respiration was inhibited. Oxygen consumption decreased by 86.7 % and 70.1 %, while, in contrast, electrical conductivity increased by 40.1 % and 36 % at 100 μg mL -1 of Genistein and Daidzein, respectively, after 48 h of exposure. The smaller number of J2s in soybean roots, the slower development rate, and the abnormal sexual differentiation were found in greenhouse assay. Thus, Genistein and Daidzein, especially Genistein, have strong effects on the physiological index of soybean cyst nematodes. Moreover, the effects were time and dosage-dependent.

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“…In general (e.g., the dependent variable is cell viability), the value of u does not exceed 100% (the formula for cell viability determines the range of this value). Similarly, l was not less than 0% (20,21). In plan A (Table 1), u and l satisfy the conditions in the model (u ≤ 100% and l ≥ 0%).…”

Section: Dose Curves Of Dex For Wound Healing In Mice With and Withou...mentioning

confidence: 92%

Different effects of a perioperative single dose of dexamethasone on wound healing in mice with or without sepsis

Chen

Zhou

2023

Front. Surg.

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IntroductionSepsis delays wound healing owing to uncontrolled inflammation. A single perioperative dose of dexamethasone is widely used because of its anti-inflammatory effects. However, the effects of dexamethasone on wound healing in sepsis remain unclear.MethodsWe discuss the methods to obtain dose curves and explore the safe dosage range for wound healing in mice with or without sepsis. A saline or LPS intraperitoneal injection was applied to C57BL/6 mice. After 24 hours, the mice received a saline or DEX intraperitoneal injection and full-thickness, dorsal wounding operation. Wound healing was observed by image record, immunofluorescence and histological staining. Inflammatory cytokines and M1/M2 macrophages in wounds were determined by ELISA and immunofluorescence, respectively.ResultsDose-response curves reflected the safe dosage range of DEX in mice with or without sepsis, from 0.121 to 2.03 mg/kg and from 0 to 0.633 mg/kg, respectively. we found that a single dose of dexamethasone (1 mg/kg, i.p.) promoted wound healing in septic mice, but delayed wound healing in normal mice. In normal mice, dexamethasone delays inflammation, resulting in an insufficient number of macrophages during the healing process. In septic mice, dexamethasone alleviated excessive inflammation and maintained the balance of M1/M2 macrophages in the early and late healing process.DiscussionIn summary, the safe dosage range of dexamethasone in septic mice is wider than that in normal mice. A single dose of dexamethasone (1 mg/kg) increased wound healing in septic mice, but delayed it in normal mice. Our findings provide helpful suggestions for the rational use of dexamethasone.

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Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XFe96 Extracellular Flux Analyzer

Cited by 22 publications

References 38 publications

Unveiling the contribution of the reproductive system of individual Caenorhabditis elegans on oxygen consumption by single-point scanning electrochemical microscopy measurements

Unveiling the contribution of the reproductive system of individual Caenorhabditis elegans on oxygen consumption by single-point scanning electrochemical microscopy measurements

Genistein and Daidzein effects on the physiological indices of Soybean Cyst Nematodes

Different effects of a perioperative single dose of dexamethasone on wound healing in mice with or without sepsis

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