Introduction: Running anaerobic sprint test (RAST) has been considered a valid test for anaerobic evaluation. However, since the floor surfaces and footwear can affect some outcomes measured during exercise, this also can modify the RAST outcomes. Objective: Thus, the purpose of this study was to compare the RAST outcomes measured while wearing soccer cleats on grass (RAST CG ) and sneakers on a track (RAST ST ). Methods: Eight young male soccer players (Under-17 category; 16±1 years) participated in the study. The subjects performed two RAST on different days (recovery > 24h). The RAST test consisted of six 35-meter maximal runs with a 10-second recovery between each run. Running time during each effort was recorded to determine peak power (PP), mean power (MP) and fatigue index (FI); blood samples were also collected after each test for lactatemia determination ([Lac]). Results: PP (763.1 ± 87.2 W) and MP (621.6 ± 68.1 W) were higher in RAST ST than RAST CG (PP = 667.3 ± 67.0 W e PM = 555.9 ± 74.7 W), while [Lac] measured during RAST ST (7.3 ± 1.8 mmol.L -1 ) was lower than measured in RAST CG (9.9 ± 3.2 mmol.L -1 ). However, the FI did not statistically differ (RAST ST = 32.5 ± 8.3%; RAST CG = 34.1 ± 6.6%). Significant correlations were observed between MP values (r = 0.90) and between [Lac] values (r = 0.72). Conclusions: Thus, we can conclude that the RAST variables are affected by floor surface and footwear, with higher values being observed during RAST ST condition.