Oxygen limitation favors the production of protein with antimicrobial activity in Pseudoalteromonas sp

Abstract

This study examined the effect of dissolved oxygen concentration on the production of biomass and metabolites with antimicrobial activity of Pseudoalteromonas sp cultured at 0, 150, 250, or 450 revolutions per minute (rev. min -1 ). Dissolved oxygen (D.O) was monitored during the fermentation process, biomass was quantified by dry weight, and antimicrobial activity was assessed using the disk diffusion method.The bacterium Pseudoalteromonas reached similar concentration of biomass under all experimental agitation conditions, whereas antimicrobial activity was detected at 0 and 150 rev. min Pseudoalteromonas is capable of growing under oxic and microaerophilic conditions but the metabolites with antimicrobial activity are induced under microaerophilic conditions.The current opinion is that Pseudoalteromonas are aerobic organisms; we provide additional information on the amount of dissolved oxygen during the fermentation process and its effect on antimicrobial activity.

The Marine Fish Gut Microbiome as a Source of Novel Bacteriocins

“…Without the correct conditions, potentially novel marine microorganisms, and thus their metabolites, may be lost when attempting to culture in vitro [85]. Environmental pressure, temperature and dissolved oxygen concentration are just a few factors to be taken into account as they can all affect the diversity of isolated microorganisms and the metabolites they produce [86][87][88][89]. Often, modified or selective media is required and long incubation times of days, or even weeks, for antimicrobial production to occur.…”