Oxygen Radical Absorbance Capacity (ORAC) and Phenolic and Anthocyanin Concentrations in Fruit and Leaf Tissues of Highbush Blueberry

Abstract

Antioxidant capacity, as measured by oxygen radical absorbance capacity (ORAC), and total phenolic and total anthocyanin contents were evaluated in fruit tissues of 87 highbush blueberry (Vacciniumcorymbosum L.) and species-introgressed highbush blueberry cultivars. ORAC and phenolic levels were evaluated in leaf tissues of the same materials. Average values for ORAC, phenolics, and anthocyanins in fruit were 15.9 ORAC units, 1.79 mg/g (gallic acid equivalents), and 0.95 mg/g (cyanidin-3-glucoside equivalents), respectively. Cv. Rubel had the highest ORAC per gram of fresh weight values, at 31.1 units, and cv. Elliott had the highest values on the basis of ORAC per square centimeter of surface area. In leaf tissue, values for both ORAC and phenolics were significantly higher than in fruit tissue, with mean values of 490 ORAC units and 44.80 mg/g (gallic acid equivalents), respectively. Leaf ORAC had a low, but significant, correlation with fruit phenolics and anthocyanins, but not with fruit ORAC. An analysis of ORAC values versus calculated midparent values in 11 plants from the 87-cultivar group in which all parents were tested suggested that, across cultivars, ORAC inheritance is additive. An investigation of ORAC values in a family of 44 cv. Rubel x Duke seedlings showed negative epistasis for ORAC values, suggesting Rubel may have gene combinations contributing to ORAC that are broken up during hybridization.

Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development

“…The content and composition of anthocyanins in bilberries, as well as in blueberries, has been determined earlier by Suomalainen and Keränen (1961), Martinelli et al (1986), and Wang et al (2000). Several studies have also been done on the bioactive properties of bilberries and blueberries (Bomser et al, 1996;Kalt and Dufour, 1997;Youdim et al, 2000), especially concerning the antioxidant activity of their anthocyanin fractions (Prior et al, 1998;Smith et al, 2000;Ehlenfeldt and Prior, 2001). However, to our knowledge, there are no published studies on flavonoid biosynthesis in blueberries with particular regard to the expression of genes in the phenyl propanoid pathway.…”

Effect ofVaccinium ashei readeLeaves on Lipid Metabolism in Otsuka Long-Evans Tokushima Fatty Rats

“…31) In addition, previous reports have indicated that BBL contains a variety of phenolic compounds, such as chlorogenic acid, quercetin, caffeic acid, and procyanidins, and has oxygen radical absorbance capacity and antileukaemic activity. [32][33][34] Therefore, it is likely that tannin, contained in BBL, partly contributes to preventing the development of obesity-induced diseases in OLETF rats. Further investigation is necessary to characterize the active components from BBL.…”

Synthesis, Electrochemical Properties and Antioxidant Activity of Hydroxy Substituted Tetraphenylporphyrins

“…[5][6] Указанные факты делают эти соединения привлекательными с точки зрения использо-вания в качестве потенциальных антиоксидантов и пока-зывают перспективы практического применения. [7][8][9] Методы определения антиоксидантной активно-сти веществ можно разделить по способу регистрации активности потенциальных антиоксидантов на: фотоме-трические, [10][11][12][13][14][15] хемилюминесцентные, [16] волюмометри-ческие, [17] флуоресцентные, [18][19][20] электрохимические [21][22] и др. ; [23,24] по типу источника окисления и окисляемого соединения.…”