Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs

Andersen, Jens Bo; Roldgaard, Bent; Christensen, Bjarke Bak; Licht, Tine Rask

doi:10.1186/1471-2180-7-55

Cited by 53 publications

(39 citation statements)

References 26 publications

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“…The importance of the anaerobic environment is supported by data from Larsen et al (101), who reported higher invasion of Caco-2 cells by L. monocytogenes grown for 2 and 4 weeks on modified atmosphere-packaged ham at 4°C than by bacteria grown in BHIB. Andersen et al (102) reported that L. monocytogenes grown under oxygen-restricted conditions were more invasive to Caco-2 cells and yielded higher bacterial loads in organs after oral guinea pig challenge than did bacteria grown without oxygen restriction. In contrast to our findings, Olesen et al (20) reported that L. monocytogenes strain O57 prfA transcript levels were significantly higher in bacteria grown in BHI than in those grown in liver pâté at 7°C.…”

Section: Figmentioning

confidence: 99%

Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Growth on Vacuum-Packed Cold Smoked Salmon

Tang

Orsi

Bakker

et al. 2015

Appl Environ Microbiol

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The foodborne pathogen Listeria monocytogenes is able to survive and grow in ready-to-eat foods, in which it is likely to experience a number of environmental stresses due to refrigerated storage and the physicochemical properties of the food. Little is known about the specific molecular mechanisms underlying survival and growth of L. monocytogenes under different complex conditions on/in specific food matrices. Transcriptome sequencing (RNA-seq) was used to understand the transcriptional landscape of L. monocytogenes strain H7858 grown on cold smoked salmon (CSS; water phase salt, 4.65%; pH 6.1) relative to that in modified brain heart infusion broth (MBHIB; water phase salt, 4.65%; pH 6.1) at 7°C. Significant differential transcription of 149 genes was observed (false-discovery rate [FDR], <0.05; fold change, >2.5), and 88 and 61 genes were up-and downregulated, respectively, in H7858 grown on CSS relative to the genes in H7858 grown in MBHIB. In spite of these differences in transcriptomes under these two conditions, growth parameters for L. monocytogenes were not significantly different between CSS and MBHIB, indicating that the transcriptomic differences reflect how L. monocytogenes is able to facilitate growth under these different conditions. Differential expression analysis and Gene Ontology enrichment analysis indicated that genes encoding proteins involved in cobalamin biosynthesis as well as ethanolamine and 1,2-propanediol utilization have significantly higher transcript levels in H7858 grown on CSS than in that grown in MBHIB. Our data identify specific transcriptional profiles of L. monocytogenes growing on vacuum-packaged CSS, which may provide targets for the development of novel and improved strategies to control L. monocytogenes growth on this ready-to-eat food.L isteria monocytogenes is a psychrotolerant foodborne pathogen that causes a potentially severe disease, listeriosis. This pathogen is of particular concern to the ready-to-eat (RTE) meat and seafood industries due to its ability to grow at temperatures as low as Ϫ0.4°C and under conditions of salt content as high as 25% (at 4°C) (1-3). Cold smoked salmon (CSS), an RTE seafood, represents a typical food product that can support the growth of L. monocytogenes from low numbers to potentially hazardous levels (4-9). The heat treatment applied during processing of CSS is not sufficient to inactivate microbes present on the raw material, including L. monocytogenes (10, 11). In addition, RTE food products, including CSS, can be contaminated with L. monocytogenes from environmental sources in processing facilities (10-13). Importantly, typical product characteristics of CSS, including pH, water activity (a w ), salt, and phenolic components, do not seem to be sufficient to control the growth of L. monocytogenes if it is present (8, 9).With the aim of developing control strategies that prevent or reduce growth of this pathogen in RTE seafood products, there is a need for a better understanding of the mechanisms that L. monocytogenes uses to sur...

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Section: Figmentioning

confidence: 99%

Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Growth on Vacuum-Packed Cold Smoked Salmon

Tang

Orsi

Bakker

et al. 2015

Appl Environ Microbiol

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“…monocytogenes ScottA, labeled with either CFP or YFP by Andersen et al (2006), 42 was used to investigate the impact of oxygen restriction on the infection potential. 43 Using a Caco-2 cell model system L. monocytogenes-CFP and L. monocytogenes-YFP, grown under oxygen restricted conditions were approximately 100-fold more invasive than corresponding cultures grown in the presence of oxygen. The oral infection of guinea pigs with mixtures (1:1 ratio) of oxygen restricted and unrestricted fluorescently labeled L. monocytogenes demonstrated that the oxygen restricted L. monocytogenes cells are significantly more invasive than their unrestricted counterparts, shown by the numbers of bacteria recovered from the liver, spleen and jejunum together with established fecal concentrations.…”

Section: Bioluminescent Imagining (Bli)mentioning

confidence: 99%

“…This study suggests that infective potential of L. monocytogenes in humans might also depend on oxygen levels encountered before ingestion, a concept that has obvious implications for food processors and packagers. 44 eGFP emits more intense light and with slightly different spectral properties when compared to GFP. eGFP based promoter reporters were utilized in a study of the SOS response in L. monocytogenes and this was presented by Stijn van der Veen genes under PrfA control appeared primarily in the blood.…”

Section: Bioluminescent Imagining (Bli)mentioning

confidence: 99%

Listeria monocytogenes

O’Byrne

Utratna

2010

Bioengineered Bugs

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“…Thiols, amines, aromatic residues, heme groups, and iron-sulfur clusters are particularly susceptible to attack by RNIs, making many key metabolic enzymes targets (17,18). UPEC may also encounter RNIs outside the urinary tract, possibly during passage through the upper gastrointestinal tract, where nitrate (NO 3 Ϫ ) and nitrite (NO 2 Ϫ ) levels can be very high, or on the surface of meat products, which are often treated with nitrite as a coloring agent and preservative (15,16,27,29,64).Adaptive responses that allow a bacterial population to survive one stressful condition can, in some instances, enhance its ability to handle other environmental stresses (2,5,31,32,42). This cross-protective effect may also potentiate bacterial virulence within a host.…”

mentioning

confidence: 99%

“…Adaptive responses that allow a bacterial population to survive one stressful condition can, in some instances, enhance its ability to handle other environmental stresses (2,5,31,32,42). This cross-protective effect may also potentiate bacterial virulence within a host.…”

mentioning

confidence: 99%

Conditioning of Uropathogenic Escherichia coli for Enhanced Colonization of Host

2009

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While in transit within and between hosts, uropathogenic Escherichia coli (UPEC) encounters multiple stresses, including substantial levels of nitric oxide and reactive nitrogen intermediates. Here we show that UPEC, the primary cause of urinary tract infections, can be conditioned to grow at higher rates in the presence of acidified sodium nitrite (ASN), a model system used to generate nitrosative stress. When inoculated into the bladder of a mouse, ASN-conditioned UPEC bacteria are far more likely to establish an infection than nonconditioned bacteria. Microarray analysis of ASN-conditioned bacteria suggests that several NsrR-regulated genes and other stress-and polyamine-responsive factors may be partially responsible for this effect. Compared to K-12 reference strains, most UPEC isolates have increased resistance to ASN, and this resistance can be substantially enhanced by addition of the polyamine cadaverine. Nitrosative stress, as generated by ASN, can stimulate cadaverine synthesis by UPEC, and growth of UPEC in cadaverine-supplemented broth in the absence of ASN can also promote UPEC colonization of the bladder. These results suggest that UPEC interactions with polyamines or stresses such as reactive nitrogen intermediates can in effect reprogram the bacteria, enabling them to better colonize the host.The urinary tract is normally a sterile environment, and it is both hostile and poorly accessible to most microbes. However, roughly one-half of women in the United States experience a urinary tract infection (UTI) at least once in their lifetime, and one-quarter of affected women endure recurrence (22,25). More than 80% of UTIs are due to strains of uropathogenic Escherichia coli (UPEC), which are usually presumed to be part-time gut flora that have reached the urinary tract by ascension via the periurethral area (53). Transmission of UPEC among individuals occurs primarily by way of fecal-oral routes and, in some cases, may involve the ingestion of contaminated food products or sexual contact (15,23,33,40,41,57). In order to survive and disseminate, UPEC must be able to adapt to multiple environments and stresses both within and outside the host.When a UPEC infection occurs, recruitment of nitric oxide (NO)-producing neutrophils to the bladder is an important line of defense (26,48). Within hours of infection, the nitrite levels in the urine increase up to threefold, and eventually the levels of NO within the bladder are 30-to 50-fold higher than those in uninfected controls (39, 48). The high levels of NO are due in part to inducible NO synthase activity, which is upregulated within 6 h after infection (45). A role may also be played by endothelial NO synthase, which is upregulated and activated in the bladder mucosa by E. coli lipopolysaccharide (36) and by the bacteria themselves, which can produce NO with nitrite reductases under low-oxygen-tension conditions (12). NO is a precursor of a variety of reactive nitrogen intermediates (RNIs), such as peroxynitrite and nitrosothiols, which can inflict exten...

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Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs

Abstract: Background: Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis.

Cited by 53 publications

References 26 publications

Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Growth on Vacuum-Packed Cold Smoked Salmon

Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Growth on Vacuum-Packed Cold Smoked Salmon

Listeria monocytogenes

Conditioning of Uropathogenic Escherichia coli for Enhanced Colonization of Host

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