Oxygen Tension Regulates Human Mesenchymal Stem Cell Paracrine Functions

Paquet, Joseph; Deschepper, Mickael; Moya, Adrien; Logeart-Avramoglou, Delphine; Boisson-Vidal, Catherine; Petite, Hervé

doi:10.5966/sctm.2014-0180

Cited by 94 publications

(82 citation statements)

References 41 publications

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“…The result of the application of MSCs was the absence of specific tubercular inflammation of the bladder caused by Mtb, which was detectable in the group without treatment (Figure , Table ). MSCs repair various injured tissues through a paracrine mechanism (Paquet et al, ; Shudo et al, ). It was shown that MSC‐derived exosomes repair the injured myocardium (Shao et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis

Yudintceva

Bogolyubova

Muraviov

et al. 2017

J Tissue Eng Regen Med

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Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.

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Section: Discussionmentioning

confidence: 99%

“…Several Table 1). MSCs repair various injured tissues through a paracrine mechanism (Paquet et al, 2015;Shudo et al, 2014). It was shown that MSC-derived exosomes repair the injured myocardium (Shao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis

Yudintceva

Bogolyubova

Muraviov

et al. 2017

J Tissue Eng Regen Med

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“…Several previous studies have reported on the capacity of MSCs to differentiate into osteogenic, chondrogenic, or adipogenic cells when cultured in hypoxic conditions [15][16][17]. However, oxygen tension may regulate human MSC paracrine functions [21], and the myocardial medium from hypoxic preconditioning can induce MSC differentiation into myocardial-like cells [33]. In our previous study, we found that a low oxygen tension induces the antiarrhythmic potential of MSCs in an animal model of myocardial infarction [24].…”

Section: Discussionmentioning

confidence: 99%

Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia

Choi¹,

Kim²,

Lee³

et al. 2016

Cell Physiol Biochem

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Background/Aims: It is known that mesenchymal stem cells (MSCs) can have variable responses to hypoxic conditions and that hypoxia may specifically stimulate differentiation into osteogenic, chondrogenic, or adipogenic cells. Based on our previous study, we hypothesized that hypoxia may also induce MSC differentiation into cardiomyocytes and/or cells with comparable phenotypes. Methods: The differences in the proteomes were specifically investigated in bone marrow-derived rat MSCs (BM-rMSCs) under normoxic and hypoxic conditions using 2-DE combined with a MALDI-TOF-MS analysis and western blot analysis. In addition, genetic and/or proteomic interactions were assessed using a String network analysis. Results: Among the 35 markedly changed spots from a total of 393 matched spots, 24 were highly up-regulated and 11 were significantly down-regulated in hypoxic rMSCs based on a proteomic analysis. Although hypoxia failed to induce the direct differentiation of rMSCs into cardiomyocytes, several cardiomyocyte differentiation-related genes and proteins were significantly increased by hypoxic stress. Conclusion: We found that BM-rMSCs alter their expression of several cardiomyocyte differentiation-related genes and proteins under hypoxic conditions, and we examined the interactions between these genes and/or proteins, providing new insights for the applicability of MSCs preconditioned by hypoxic stimulation for use in cardiac diseases.

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“…18,74 In line, is it shown that MSCs ageing significantly alters telomerase length, differentiation potential, proliferation index, and secretome along with the induction of a senescent phenotype in culture. 75,76 Moreover, changes in oxygen tension can modulate paracrine functions of MSCs 77 and it is likely MSC grown under variable oxygen tensions might have exosome cargo differences. Therefore, it is crucial to use MSCs at low passage, and controlled growth conditions to obtain consistent results with their exosomes.…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Role of stem cell derived exosomes in tumor biology

Sharma¹

2017

Intl Journal of Cancer

105

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Exosomes are nano-scale messengers loaded with bio-molecular cargo of RNA, DNA, and Proteins. As a master regulator of cellular signaling, stem cell (both normal, and cancer stem cells) secreted exosome orchestrate various autocrine and paracrine functions which alter tumor micro-environment, growth and progression. Exosomes secreted by one of the two important stem cell phenotypes in cancers a) Mesenchymal stem cells, and b) Cancer stem cells not only promote cancerous growth but also impart therapy resistance in cancer cells. In tumors, normal or mesenchymal stem cell (MSCs) derived exosomes (MSC-exo) modulate tumor hallmarks by delivering unique miRNA species to neighboring cells and help in tumor progression. Apart from regulating tumor cell fate, MSC-exo are also capable of inducing physiological processes, for example, angiogenesis, metastasis and so forth. Similarly, cancer stem cells (CSCs) derived exosomes (CSC-exo) contain stemness-specific proteins, self-renewal promoting regulatory miRNAs, and survival factors. CSC-exo specific cargo maintains tumor heterogeneity and alters tumor progression. In this review we critically discuss the importance of stem cell specific exosomes in tumor cell signaling pathways with their role in tumor biology.

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Oxygen Tension Regulates Human Mesenchymal Stem Cell Paracrine Functions

Cited by 94 publications

References 41 publications

Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis

Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis

Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia

Role of stem cell derived exosomes in tumor biology

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