Oxylipin Profiling of the Hypersensitive Response in<i>Arabidopsis thaliana</i>

Andersson, Mats X.; Hámberg, Mats; Kourtchenko, Olga; Brunnström, Åsa; McPhail, Kerry L.; Gerwick, William H.; Göbel, Cornelia; Feußner, Ivo; Ellerström, Mats

doi:10.1074/jbc.m604820200

Cited by 123 publications

(72 citation statements)

References 42 publications

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“…Second, fenclorim (or a downstream metabolite) could activate a minor release of oxylipins leading to signal elicitation. A candidate enzyme for such bioactivation would be a lipase with many plants including Arabidopsis, accumulating relatively large amounts of esterified OPDA and phytoprostanes in lipids (44). If the safeners were to cause a selective release of such preformed stores through the up-regulation of hydrolytic enzymes, this would potentially lead to an RXR.…”

Section: Discussionmentioning

confidence: 99%

Xenobiotic Responsiveness of Arabidopsis thaliana to a Chemical Series Derived from a Herbicide Safener

Skipsey¹,

Knight

Brazier-Hicks³

et al. 2011

Journal of Biological Chemistry

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Plants respond to synthetic chemicals by eliciting a xenobiotic response (XR) that enhances the expression of detoxifying enzymes such as glutathione transferases (GSTs). In agrochemistry, the ability of safeners to induce an XR is used to increase herbicide detoxification in cereal crops. Based on the responsiveness of the model plant Arabidopsis thaliana to the rice safener fenclorim (4,6-dichloro-2-phenylpyrimidine), a series of related derivatives was prepared and tested for the ability to induce GSTs in cell suspension cultures. The XR in Arabidopsis could be divided into rapid and slow types depending on subtle variations in the reactivity (electrophilicity) and chemical structure of the derivatives. In a comparative microarray study, Arabidopsis cultures were treated with closely related compounds that elicited rapid (fenclorim) and slow (4-chloro-6-methyl-2-phenylpyrimidine) XRs. Both chemicals induced major changes in gene expression, including a coordinated suppression in cell wall biosynthesis and an up-regulation in detoxification pathways, whereas only fenclorim selectively induced sulfur and phenolic metabolism. These transcriptome studies suggested several linkages between the XR and oxidative and oxylipin signaling. Confirming links with abiotic stress signaling, suppression of glutathione content enhanced GST induction by fenclorim, whereas fatty acid desaturase mutants, which were unable to synthesize oxylipins, showed an attenuated XR. Examining the significance of these studies to agrochemistry, only those fenclorim derivatives that elicited a rapid XR proved effective in increasing herbicide tolerance (safening) in rice.

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Section: Discussionmentioning

confidence: 99%

Xenobiotic Responsiveness of Arabidopsis thaliana to a Chemical Series Derived from a Herbicide Safener

Skipsey¹,

Knight

Brazier-Hicks³

et al. 2011

Journal of Biological Chemistry

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“…Recently a trioxylipin MGDG species has been identiWed that accumulates in C. Böttcher (&) · E. W. Weiler Lehrstuhl für PXanzenphysiologie, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany e-mail: christine.boettcher@rub.de response to a bacterial avirulence protein (Andersson et al 2006). But all in all the biological role(s), of this class of oxylipin-containing lipids in the plant remain(s) mainly unclear.…”

Section: Introductionmentioning

confidence: 98%

cyclo-Oxylipin-galactolipids in plants: occurrence and dynamics

Böttcher

Weiler

2007

Planta

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cyclo-Oxylipin-galactolipids (cGL) are mono- or digalactosyldiglycerides carrying a cyclo-oxylipin in the sn1- and/or sn2-position or esterified to the galactose moiety. These compounds were recently identified in Arabidopsis thaliana. We provide evidence that cGL are mainly, if not exclusively, part of the thylakoid and can be hydrolysed by lipolytic activities associated with photosynthesis-related protein complexes in vitro. Using HPLC/ESI-mass spectrometry, cGL are shown to be restricted in occurrence to the genus Arabidopsis, they do not occur in other plants tested. A. thaliana cGL are rapidly and transiently formed upon wounding with characteristic changes in composition of the cGL-fraction. While the biological role of cGL is not understood, the genus Arabidopsis may present a model-case of chemical evolution of a novel class of regulatory molecules.

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“…This species was given the common name "Arabidopside E". 9,10 It is now apparent that a wide variety of acylated MGDG molecular species and DGMGs are produced upon wounding of Arabidopsis leaves (Fig. 1C and D).…”

Section: Overviewmentioning

confidence: 99%