Oxytocin Ameliorates Remote Liver Injury Induced by Renal Ischemia-Reperfusion in Rats

Abstract

Renal ischemia-reperfusion (IR) causes remote liver damage. Oxytocin has anti-inflammatory and antioxidant effects. The main purpose of this study was to evaluate the protective function of oxytocin (OT) in remote liver damage triggered by renal IR in rats. Twenty four rats were randomly divided into four different groups, each containing 8 rats. The groups were as follows: (1) Sham operated group; (2) Sham operated+OT group (3) Renal IR group; (4) Renal IR+OT group. OT (500µg/kg) was administered subcutaneously 12 and 24 hours before and immediately after ischemia. At the end of experimental procedure, the rats were sacrificed, and liver specimens were taken for histological assessment or determination of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), paraoxonase (PON-1) activity and nitric oxide (NO). The results showed that renal IR injury constituted a notable elevation in MDA, TOS, Oxidative stress index (OSI) and significantly decreased TAS, PON-1 actvity and NO in liver tissue (p<0.05). Additionally renal IR provoked significant augmentation in hepatic microscopic damage scores. However, alterations in these biochemical and histopathological indices due to IR injury were attenuated by OT treatment (p<0.05). These findings show that OT ameliorates remote liver damage triggered by renal ischemia-reperfusion and this preservation involves suppression of inflammation and regulation of oxidant-antioxidant status.

Protective Effect of p-Coumaric Acid as Free Oxygen Radical Scavenger in Experimental Renal Ischemia-Reperfusion Model

“…4,5 Acute renal insuffi ciency occurs in approximately 5% of hospitalized patients and 30% of patients in intensive care units. 5,6 Although the pathophysiology of R-IR injury is complex; basically increased infl ammation plays a major role in the responsibility. 7 Severe responses in ischemia, including activation of reactive oxygen species (ROS), neutrophils, infl ammatory mediators such as cytokines and adhesion molecules, play a role in the onset of R-IR injury.…”

Effects of different periods of gastric ischemia on liver as a remote organ

“…Gastric ischemia, such as the gut ischemia, unleashes a distributive shock due to the increase in the vascular permeability and reduction in aorta blood flow 9 and a local inflammatory process which extends to other organs and can be prolonged, giving rise to the onset of MODS 10 . Liver failure is a common complication in critical diseases and contributes to the worsening of patient prognosis [10][11][12][13][14][15] . The liver plays an important role in the immune, endocrine, and metabolic control, and is frequently damaged in medical conditions of shock, sepsis and systemic inflammation.…”

Oxytocin effects on experimental skin wound healing

“…This might be due to the fact of less concentrations of tumor necrosis factor-α and interleukin-6, a decreased rate of apoptosis, diminished neutrophil recruitment, and less lipid peroxidation in the different organs under investigation [51]. In this context, OXY has been described to act as an anti-inflammatory agent via the increase of corticosterone levels even after a single OXY injection [7,14,15,55] as well as an antioxidant via the regulation of the oxidant-antioxidant status [52,53,56,57]. Furthermore, the anti-inflammatory OXY action was recently been reported to be a direct neutrophil-dependent mechanism, ameliorating the oxidative organ damage [52,58].…”